Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase [in yeast Saccharomyces cerevisiae]
The region of the mtDNA containing the structural gene for apocytochrome b is called the cob or box region. There is evidence that the same region is also involved in the regulation of cytochrome oxidase. We have isolated eight mit- mutants in this region and have ordered them using petite deletion...
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| Veröffentlicht in: | The Journal of biological chemistry 1979-04, Vol.254 (7), p.2471-2479 |
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| container_issue | 7 |
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| container_title | The Journal of biological chemistry |
| container_volume | 254 |
| creator | Alexander, N.J Vincent, R.D Perlman, P.S Miller, D.H Hanson, D.K Mahler, H.R |
| description | The region of the mtDNA containing the structural gene for apocytochrome b is called the cob or box region. There is evidence
that the same region is also involved in the regulation of cytochrome oxidase. We have isolated eight mit- mutants in this
region and have ordered them using petite deletion mapping. Four of these mutants appear to map outside the boxII region on
the oli2-proximal end. Analysis of restriction endonuclease fragments of the mtDNA from peptides used in the deletion mapping
suggests a minimum size of 3.1 x 10(3) base pairs for the whole cob region. Although none of our mutants contained any cytochrome
b or cytochrome b-linked activities, polypeptides apparently related to apocytochrome b were present in some but not all mutants.
Additional regulatory effects (both positive and negative) on cytochrome oxidase by virtue of control of its subunit I were
also observed. In addition to these phenotypic traits, some of the mutants accumulated novel, mitochondrially translated polypeptides
not seen in wild type. |
| doi_str_mv | 10.1016/S0021-9258(17)30247-8 |
| format | Article |
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that the same region is also involved in the regulation of cytochrome oxidase. We have isolated eight mit- mutants in this
region and have ordered them using petite deletion mapping. Four of these mutants appear to map outside the boxII region on
the oli2-proximal end. Analysis of restriction endonuclease fragments of the mtDNA from peptides used in the deletion mapping
suggests a minimum size of 3.1 x 10(3) base pairs for the whole cob region. Although none of our mutants contained any cytochrome
b or cytochrome b-linked activities, polypeptides apparently related to apocytochrome b were present in some but not all mutants.
Additional regulatory effects (both positive and negative) on cytochrome oxidase by virtue of control of its subunit I were
also observed. In addition to these phenotypic traits, some of the mutants accumulated novel, mitochondrially translated polypeptides
not seen in wild type.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)30247-8</identifier><identifier>PMID: 218939</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Apoproteins - biosynthesis ; Cytochromes - biosynthesis ; DNA Restriction Enzymes ; DNA, Mitochondrial - metabolism ; Electron Transport Complex IV - biosynthesis ; Genes ; Mutation ; Protein Biosynthesis ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Species Specificity ; Transcription, Genetic</subject><ispartof>The Journal of biological chemistry, 1979-04, Vol.254 (7), p.2471-2479</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3118-852b191dd65c0f9171280b397ca62b414da7c018d6ee7b953ea948bce8c737fa3</citedby><cites>FETCH-LOGICAL-c3118-852b191dd65c0f9171280b397ca62b414da7c018d6ee7b953ea948bce8c737fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/218939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alexander, N.J</creatorcontrib><creatorcontrib>Vincent, R.D</creatorcontrib><creatorcontrib>Perlman, P.S</creatorcontrib><creatorcontrib>Miller, D.H</creatorcontrib><creatorcontrib>Hanson, D.K</creatorcontrib><creatorcontrib>Mahler, H.R</creatorcontrib><title>Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase [in yeast Saccharomyces cerevisiae]</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The region of the mtDNA containing the structural gene for apocytochrome b is called the cob or box region. There is evidence
that the same region is also involved in the regulation of cytochrome oxidase. We have isolated eight mit- mutants in this
region and have ordered them using petite deletion mapping. Four of these mutants appear to map outside the boxII region on
the oli2-proximal end. Analysis of restriction endonuclease fragments of the mtDNA from peptides used in the deletion mapping
suggests a minimum size of 3.1 x 10(3) base pairs for the whole cob region. Although none of our mutants contained any cytochrome
b or cytochrome b-linked activities, polypeptides apparently related to apocytochrome b were present in some but not all mutants.
Additional regulatory effects (both positive and negative) on cytochrome oxidase by virtue of control of its subunit I were
also observed. In addition to these phenotypic traits, some of the mutants accumulated novel, mitochondrially translated polypeptides
not seen in wild type.</description><subject>Apoproteins - biosynthesis</subject><subject>Cytochromes - biosynthesis</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Mitochondrial - metabolism</subject><subject>Electron Transport Complex IV - biosynthesis</subject><subject>Genes</subject><subject>Mutation</subject><subject>Protein Biosynthesis</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Species Specificity</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkd2KFDEQhRvxb1x9AVEIXohe9Jjqv6QvZdF1YUFwXBBEQnW6eiYy3ZlN0q7tI_pUpqeHxdxUSH11DqmTJC-Br4FD9W7DeQZpnZXyDYi3Oc8Kkcp7yQq4zNO8hG_3k9Ud8jh54v1PHk9Rw6PkYQayzutV8vcLbcc9BusmZoZADnUwdvCsoXBLNLDeBKt3dmidwT3b0kB-zS7X7CLegtEMh5Y1JiLUGx0JvcNZg5z5g7MSsx3ztifWjwGHwMJ0IM-w6ygaDVuGB6un2cLNUHPU--_B_jYtemLfzcAmQh_YBvXsYftJRyFNjn4Zb5B-PE0edLj39OxUz5Lrjx--nn9Krz5fXJ6_v0p1DiBTWWYN1NC2Val5V4OATPImr4XGKmsKKFoUmoNsKyLR1GVOWBey0SS1yEWH-VnyetE9OHszkg-qN17Tfo8D2dErURRlWUERwXIBtbPeO-rUwZke3aSAqzlCdYxQzfkoEOoYoZJx7sXJYGx6au-mlsxi-9XS3pnt7tY4Uqf9q6wslFBRBCL0fIE6tAq3znh1vRF1_F4h8n_7-LDL</recordid><startdate>19790410</startdate><enddate>19790410</enddate><creator>Alexander, N.J</creator><creator>Vincent, R.D</creator><creator>Perlman, P.S</creator><creator>Miller, D.H</creator><creator>Hanson, D.K</creator><creator>Mahler, H.R</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19790410</creationdate><title>Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase [in yeast Saccharomyces cerevisiae]</title><author>Alexander, N.J ; Vincent, R.D ; Perlman, P.S ; Miller, D.H ; Hanson, D.K ; Mahler, H.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3118-852b191dd65c0f9171280b397ca62b414da7c018d6ee7b953ea948bce8c737fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Apoproteins - biosynthesis</topic><topic>Cytochromes - biosynthesis</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Mitochondrial - metabolism</topic><topic>Electron Transport Complex IV - biosynthesis</topic><topic>Genes</topic><topic>Mutation</topic><topic>Protein Biosynthesis</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Species Specificity</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alexander, N.J</creatorcontrib><creatorcontrib>Vincent, R.D</creatorcontrib><creatorcontrib>Perlman, P.S</creatorcontrib><creatorcontrib>Miller, D.H</creatorcontrib><creatorcontrib>Hanson, D.K</creatorcontrib><creatorcontrib>Mahler, H.R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alexander, N.J</au><au>Vincent, R.D</au><au>Perlman, P.S</au><au>Miller, D.H</au><au>Hanson, D.K</au><au>Mahler, H.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase [in yeast Saccharomyces cerevisiae]</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1979-04-10</date><risdate>1979</risdate><volume>254</volume><issue>7</issue><spage>2471</spage><epage>2479</epage><pages>2471-2479</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The region of the mtDNA containing the structural gene for apocytochrome b is called the cob or box region. There is evidence
that the same region is also involved in the regulation of cytochrome oxidase. We have isolated eight mit- mutants in this
region and have ordered them using petite deletion mapping. Four of these mutants appear to map outside the boxII region on
the oli2-proximal end. Analysis of restriction endonuclease fragments of the mtDNA from peptides used in the deletion mapping
suggests a minimum size of 3.1 x 10(3) base pairs for the whole cob region. Although none of our mutants contained any cytochrome
b or cytochrome b-linked activities, polypeptides apparently related to apocytochrome b were present in some but not all mutants.
Additional regulatory effects (both positive and negative) on cytochrome oxidase by virtue of control of its subunit I were
also observed. In addition to these phenotypic traits, some of the mutants accumulated novel, mitochondrially translated polypeptides
not seen in wild type.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>218939</pmid><doi>10.1016/S0021-9258(17)30247-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1979-04, Vol.254 (7), p.2471-2479 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74455614 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Apoproteins - biosynthesis Cytochromes - biosynthesis DNA Restriction Enzymes DNA, Mitochondrial - metabolism Electron Transport Complex IV - biosynthesis Genes Mutation Protein Biosynthesis Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Species Specificity Transcription, Genetic |
| title | Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase [in yeast Saccharomyces cerevisiae] |
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