Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system

The inducible, mannitol-specific Enzyme II of the phosphoenolpyruvate:sugar phosphotransferase system has been purified approximately 230-fold from Escherichia coli membranes. The enzyme, initially solubilized with deoxycholate, was first subjected to hydrophobic chromatography on hexyl agarose and...

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Veröffentlicht in:	The Journal of biological chemistry 1979-01, Vol.254 (2), p.249-252
Hauptverfasser:	G R Jacobson, C A Lee, M H Saier, Jr
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli - enzymology Mannitol Molecular Weight Phosphoenolpyruvate Phosphotransferases - isolation & purification
Online-Zugang:	Volltext
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