Comparison of susceptibility to staphylococcal nuclease and behaviour in metrizamide gradients of normal and 5-bromodeoxyuridine-substituted chromatin from Physarum polycephalum

Replacement of 20--30% of thymine by 5-bromodeoxyuridine in chromatin DNA of Physarum polycephalum does not cause any visible change in a typical, regular pattern of DNA products obtained upon digestion of chromatin with staphylococcal nuclease. The time course of digestion is similar for normal and...

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Veröffentlicht in:	Acta biochimica polonica 1978, Vol.25 (3), p.273-280
Hauptverfasser:	Tyniec, B, Staroń, K, Jerzmanowski, A, Toczko, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Bromodeoxyuridine - metabolism Centrifugation, Density Gradient - methods Chromatin - metabolism Chromatin - ultrastructure DNA - metabolism DNA Replication Kinetics Metrizamide Micrococcal Nuclease - metabolism Physarum
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creator	Tyniec, B Staroń, K Jerzmanowski, A Toczko, K
description	Replacement of 20--30% of thymine by 5-bromodeoxyuridine in chromatin DNA of Physarum polycephalum does not cause any visible change in a typical, regular pattern of DNA products obtained upon digestion of chromatin with staphylococcal nuclease. The time course of digestion is similar for normal and substituted chromatin even under conditions when the nuclease cleaves preferentially the dAT regions in DNA. 5-Bromodeoxyuridine label does not significantly affect the DNA/protein ratio in chromatin; this is reflected by similar behaviour of normal and substituted chromatin in metrizamide-density gradients.
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The time course of digestion is similar for normal and substituted chromatin even under conditions when the nuclease cleaves preferentially the dAT regions in DNA. 5-Bromodeoxyuridine label does not significantly affect the DNA/protein ratio in chromatin; this is reflected by similar behaviour of normal and substituted chromatin in metrizamide-density gradients.</description><identifier>ISSN: 0001-527X</identifier><identifier>PMID: 752203</identifier><language>eng</language><publisher>Poland</publisher><subject>Bromodeoxyuridine - metabolism ; Centrifugation, Density Gradient - methods ; Chromatin - metabolism ; Chromatin - ultrastructure ; DNA - metabolism ; DNA Replication ; Kinetics ; Metrizamide ; Micrococcal Nuclease - metabolism ; Physarum</subject><ispartof>Acta biochimica polonica, 1978, Vol.25 (3), p.273-280</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/752203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tyniec, B</creatorcontrib><creatorcontrib>Staroń, K</creatorcontrib><creatorcontrib>Jerzmanowski, A</creatorcontrib><creatorcontrib>Toczko, K</creatorcontrib><title>Comparison of susceptibility to staphylococcal nuclease and behaviour in metrizamide gradients of normal and 5-bromodeoxyuridine-substituted chromatin from Physarum polycephalum</title><title>Acta biochimica polonica</title><addtitle>Acta Biochim Pol</addtitle><description>Replacement of 20--30% of thymine by 5-bromodeoxyuridine in chromatin DNA of Physarum polycephalum does not cause any visible change in a typical, regular pattern of DNA products obtained upon digestion of chromatin with staphylococcal nuclease. 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The time course of digestion is similar for normal and substituted chromatin even under conditions when the nuclease cleaves preferentially the dAT regions in DNA. 5-Bromodeoxyuridine label does not significantly affect the DNA/protein ratio in chromatin; this is reflected by similar behaviour of normal and substituted chromatin in metrizamide-density gradients.</abstract><cop>Poland</cop><pmid>752203</pmid><tpages>8</tpages></addata></record>
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subjects	Bromodeoxyuridine - metabolism Centrifugation, Density Gradient - methods Chromatin - metabolism Chromatin - ultrastructure DNA - metabolism DNA Replication Kinetics Metrizamide Micrococcal Nuclease - metabolism Physarum
title	Comparison of susceptibility to staphylococcal nuclease and behaviour in metrizamide gradients of normal and 5-bromodeoxyuridine-substituted chromatin from Physarum polycephalum
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