Sequence specificity of the P1 modification methylase (M· Eco P1) and the DNA methylase (M· Eco dam) controlled by the Escherichia coli dam gene
Labeled oligonucleotides have been fractionated from pancreatic DNase digests of DNA that had been methylated in vitro with the P1 modification enzyme (M· Eco P1) or with the DNA-adenine methylase (M· Eco dam) controlled by the Escherichia coli dam gene. The sequences of methylated oligonucleotides...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular biology 1978-12, Vol.126 (3), p.367-380 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 380 |
|---|---|
| container_issue | 3 |
| container_start_page | 367 |
| container_title | Journal of molecular biology |
| container_volume | 126 |
| creator | Hattman, Stanley Brooks, Joan E. Masurekar, Malthi |
| description | Labeled oligonucleotides have been fractionated from pancreatic DNase digests of DNA that had been methylated
in vitro with the P1 modification enzyme (M·
Eco P1) or with the DNA-adenine methylase (M·
Eco dam) controlled by the
Escherichia coli dam gene. The sequences of methylated oligonucleotides were established for M·
Eco dam modification of calf thymus DNA. The results show that M·
Eco dam inethylates adenine residues contained in the twofold symmetrical sequence, 5′ … G-A-T-C … 3′. The sequence for the site methylated by M·
Eco P1 has also been deduced; we propose that M·
Eco P1 modification produces the following methylated pentameric sequence: 5′ … A-G-A
∗-C-Py … 3′ (where
A
∗
= N
6
methyladenine and Py is C or T). |
| doi_str_mv | 10.1016/0022-2836(78)90046-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74377228</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0022283678900463</els_id><sourcerecordid>74377228</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-fce278e57b2c7a2ddd07e781d6db6021ca9be27ccef8e9243151bcf8a28510b3</originalsourceid><addsrcrecordid>eNp9kc1O4zAUha3R_HUKb8DCqxFdhPFPErsbJASFQeJPgr3lXN9Qj5K42OlIfQ1ehv08GUmLWI1YXeme7x7pnkPIAWdHnPHyF2NCZELL8lDp2ZyxvMzkJzLhTM8zXUr9mUzeke_kR0p_GGOFzPU38lUqljMxIc_3-LTGDpCmFYKvPfh-Q0NN-yXSO07b4Mal7X3oaIv9ctPYhPTw-t8LXUAYkBm1ndviZzcn_0OcbWcUQtfH0DToaLXZ0osES4welt4OauNHjj5ih3vkS22bhPtvc0oezhcPp7-zq9uLy9OTqwxkUfZZDSiUxkJVApQVzjmmUGnuSleVTHCw82ogALDWOBe55AWvoNZW6IKzSk7Jz53tKoYhgtSb1ifAprEdhnUyKpdKiSG7Kcl3IMSQUsTarKJvbdwYzsxYhBlTNmPKRmmzLcLI4ezgzX9dtejej3bJD_LxTsbhxb8eo0ngxyKcjwi9ccF_7P8KmDSYrg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74377228</pqid></control><display><type>article</type><title>Sequence specificity of the P1 modification methylase (M· Eco P1) and the DNA methylase (M· Eco dam) controlled by the Escherichia coli dam gene</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Hattman, Stanley ; Brooks, Joan E. ; Masurekar, Malthi</creator><creatorcontrib>Hattman, Stanley ; Brooks, Joan E. ; Masurekar, Malthi</creatorcontrib><description>Labeled oligonucleotides have been fractionated from pancreatic DNase digests of DNA that had been methylated
in vitro with the P1 modification enzyme (M·
Eco P1) or with the DNA-adenine methylase (M·
Eco dam) controlled by the
Escherichia coli dam gene. The sequences of methylated oligonucleotides were established for M·
Eco dam modification of calf thymus DNA. The results show that M·
Eco dam inethylates adenine residues contained in the twofold symmetrical sequence, 5′ … G-A-T-C … 3′. The sequence for the site methylated by M·
Eco P1 has also been deduced; we propose that M·
Eco P1 modification produces the following methylated pentameric sequence: 5′ … A-G-A
∗-C-Py … 3′ (where
A
∗
= N
6
methyladenine and Py is C or T).</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(78)90046-3</identifier><identifier>PMID: 370402</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Base Sequence ; Deoxyribonucleotides - analysis ; DNA ; DNA (Cytosine-5-)-Methyltransferases ; Escherichia coli - genetics ; Methyltransferases ; Nucleic Acid Denaturation ; Oligonucleotides - analysis</subject><ispartof>Journal of molecular biology, 1978-12, Vol.126 (3), p.367-380</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-fce278e57b2c7a2ddd07e781d6db6021ca9be27ccef8e9243151bcf8a28510b3</citedby><cites>FETCH-LOGICAL-c356t-fce278e57b2c7a2ddd07e781d6db6021ca9be27ccef8e9243151bcf8a28510b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0022283678900463$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/370402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hattman, Stanley</creatorcontrib><creatorcontrib>Brooks, Joan E.</creatorcontrib><creatorcontrib>Masurekar, Malthi</creatorcontrib><title>Sequence specificity of the P1 modification methylase (M· Eco P1) and the DNA methylase (M· Eco dam) controlled by the Escherichia coli dam gene</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Labeled oligonucleotides have been fractionated from pancreatic DNase digests of DNA that had been methylated
in vitro with the P1 modification enzyme (M·
Eco P1) or with the DNA-adenine methylase (M·
Eco dam) controlled by the
Escherichia coli dam gene. The sequences of methylated oligonucleotides were established for M·
Eco dam modification of calf thymus DNA. The results show that M·
Eco dam inethylates adenine residues contained in the twofold symmetrical sequence, 5′ … G-A-T-C … 3′. The sequence for the site methylated by M·
Eco P1 has also been deduced; we propose that M·
Eco P1 modification produces the following methylated pentameric sequence: 5′ … A-G-A
∗-C-Py … 3′ (where
A
∗
= N
6
methyladenine and Py is C or T).</description><subject>Base Sequence</subject><subject>Deoxyribonucleotides - analysis</subject><subject>DNA</subject><subject>DNA (Cytosine-5-)-Methyltransferases</subject><subject>Escherichia coli - genetics</subject><subject>Methyltransferases</subject><subject>Nucleic Acid Denaturation</subject><subject>Oligonucleotides - analysis</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1O4zAUha3R_HUKb8DCqxFdhPFPErsbJASFQeJPgr3lXN9Qj5K42OlIfQ1ehv08GUmLWI1YXeme7x7pnkPIAWdHnPHyF2NCZELL8lDp2ZyxvMzkJzLhTM8zXUr9mUzeke_kR0p_GGOFzPU38lUqljMxIc_3-LTGDpCmFYKvPfh-Q0NN-yXSO07b4Mal7X3oaIv9ctPYhPTw-t8LXUAYkBm1ndviZzcn_0OcbWcUQtfH0DToaLXZ0osES4welt4OauNHjj5ih3vkS22bhPtvc0oezhcPp7-zq9uLy9OTqwxkUfZZDSiUxkJVApQVzjmmUGnuSleVTHCw82ogALDWOBe55AWvoNZW6IKzSk7Jz53tKoYhgtSb1ifAprEdhnUyKpdKiSG7Kcl3IMSQUsTarKJvbdwYzsxYhBlTNmPKRmmzLcLI4ezgzX9dtejej3bJD_LxTsbhxb8eo0ngxyKcjwi9ccF_7P8KmDSYrg</recordid><startdate>19781215</startdate><enddate>19781215</enddate><creator>Hattman, Stanley</creator><creator>Brooks, Joan E.</creator><creator>Masurekar, Malthi</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19781215</creationdate><title>Sequence specificity of the P1 modification methylase (M· Eco P1) and the DNA methylase (M· Eco dam) controlled by the Escherichia coli dam gene</title><author>Hattman, Stanley ; Brooks, Joan E. ; Masurekar, Malthi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-fce278e57b2c7a2ddd07e781d6db6021ca9be27ccef8e9243151bcf8a28510b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Base Sequence</topic><topic>Deoxyribonucleotides - analysis</topic><topic>DNA</topic><topic>DNA (Cytosine-5-)-Methyltransferases</topic><topic>Escherichia coli - genetics</topic><topic>Methyltransferases</topic><topic>Nucleic Acid Denaturation</topic><topic>Oligonucleotides - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hattman, Stanley</creatorcontrib><creatorcontrib>Brooks, Joan E.</creatorcontrib><creatorcontrib>Masurekar, Malthi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hattman, Stanley</au><au>Brooks, Joan E.</au><au>Masurekar, Malthi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence specificity of the P1 modification methylase (M· Eco P1) and the DNA methylase (M· Eco dam) controlled by the Escherichia coli dam gene</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1978-12-15</date><risdate>1978</risdate><volume>126</volume><issue>3</issue><spage>367</spage><epage>380</epage><pages>367-380</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>Labeled oligonucleotides have been fractionated from pancreatic DNase digests of DNA that had been methylated
in vitro with the P1 modification enzyme (M·
Eco P1) or with the DNA-adenine methylase (M·
Eco dam) controlled by the
Escherichia coli dam gene. The sequences of methylated oligonucleotides were established for M·
Eco dam modification of calf thymus DNA. The results show that M·
Eco dam inethylates adenine residues contained in the twofold symmetrical sequence, 5′ … G-A-T-C … 3′. The sequence for the site methylated by M·
Eco P1 has also been deduced; we propose that M·
Eco P1 modification produces the following methylated pentameric sequence: 5′ … A-G-A
∗-C-Py … 3′ (where
A
∗
= N
6
methyladenine and Py is C or T).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>370402</pmid><doi>10.1016/0022-2836(78)90046-3</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2836 |
| ispartof | Journal of molecular biology, 1978-12, Vol.126 (3), p.367-380 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74377228 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Base Sequence Deoxyribonucleotides - analysis DNA DNA (Cytosine-5-)-Methyltransferases Escherichia coli - genetics Methyltransferases Nucleic Acid Denaturation Oligonucleotides - analysis |
| title | Sequence specificity of the P1 modification methylase (M· Eco P1) and the DNA methylase (M· Eco dam) controlled by the Escherichia coli dam gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A30%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequence%20specificity%20of%20the%20P1%20modification%20methylase%20(M%C2%B7%20Eco%20P1)%20and%20the%20DNA%20methylase%20(M%C2%B7%20Eco%20dam)%20controlled%20by%20the%20Escherichia%20coli%20dam%20gene&rft.jtitle=Journal%20of%20molecular%20biology&rft.au=Hattman,%20Stanley&rft.date=1978-12-15&rft.volume=126&rft.issue=3&rft.spage=367&rft.epage=380&rft.pages=367-380&rft.issn=0022-2836&rft.eissn=1089-8638&rft_id=info:doi/10.1016/0022-2836(78)90046-3&rft_dat=%3Cproquest_cross%3E74377228%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74377228&rft_id=info:pmid/370402&rft_els_id=0022283678900463&rfr_iscdi=true |