Est1 and Cdc13 as Comediators of Telomerase Access
Cdc13 and Est1 are single-strand telomeric DNA binding proteins that contribute to telomere replication in the yeast Saccharomyces cerevisiae. Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1999-10, Vol.286 (5437), p.117-120 |
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| creator | Evans, Sara K. Lundblad, Victoria |
| description | Cdc13 and Est1 are single-strand telomeric DNA binding proteins that contribute to telomere replication in the yeast Saccharomyces cerevisiae. Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant versions of Cdc13 or Est1 confer similar telomere elongation, indicating that close physical proximity can bypass telomerase-defective mutations in either protein. Fusing Cdc13 directly to the catalytic core of telomerase allows stable-telomere maintenance in the absence of Est1, consistent with a role for Est1 in mediating telomerase access. Telomere length homeostasis therefore is maintained in part by restricting access of telomerase to chromosome termini, but this limiting situation can be overcome by directly tethering telomerase to the telomere. |
| doi_str_mv | 10.1126/science.286.5437.117 |
| format | Article |
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Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant versions of Cdc13 or Est1 confer similar telomere elongation, indicating that close physical proximity can bypass telomerase-defective mutations in either protein. Fusing Cdc13 directly to the catalytic core of telomerase allows stable-telomere maintenance in the absence of Est1, consistent with a role for Est1 in mediating telomerase access. Telomere length homeostasis therefore is maintained in part by restricting access of telomerase to chromosome termini, but this limiting situation can be overcome by directly tethering telomerase to the telomere.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.286.5437.117</identifier><identifier>PMID: 10506558</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Alleles ; Binding Sites ; Biological and medical sciences ; Cell growth ; Cellular senescence ; Chromatin. Chromosome ; Cyclin B - genetics ; Cyclin B - metabolism ; Deoxyribonucleic acid ; DNA ; DNA binding proteins ; DNA, Fungal - metabolism ; DNA, Single-Stranded - metabolism ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Genetic Complementation Test ; Genetic mutation ; Genetics ; Homeostasis ; Models, Biological ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Mutation (Biology) ; Plasmids ; Proteins ; Proximity ; Recombinant Fusion Proteins - metabolism ; RNA ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; Sprains and strains ; Telomerase ; Telomerase - genetics ; Telomerase - metabolism ; Telomere - metabolism ; Telomeres ; Yeast</subject><ispartof>Science (American Association for the Advancement of Science), 1999-10, Vol.286 (5437), p.117-120</ispartof><rights>Copyright 1999 American Association for the Advancement of Science</rights><rights>1999 INIST-CNRS</rights><rights>COPYRIGHT 1999 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1999 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Oct 1, 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c783t-384d733f136687156e2f3f7f73f3f3ae0a20c84ba962a87a820e4c1e98ac36093</citedby><cites>FETCH-LOGICAL-c783t-384d733f136687156e2f3f7f73f3f3ae0a20c84ba962a87a820e4c1e98ac36093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2898904$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2898904$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1972107$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10506558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Evans, Sara K.</creatorcontrib><creatorcontrib>Lundblad, Victoria</creatorcontrib><title>Est1 and Cdc13 as Comediators of Telomerase Access</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Cdc13 and Est1 are single-strand telomeric DNA binding proteins that contribute to telomere replication in the yeast Saccharomyces cerevisiae. Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant versions of Cdc13 or Est1 confer similar telomere elongation, indicating that close physical proximity can bypass telomerase-defective mutations in either protein. Fusing Cdc13 directly to the catalytic core of telomerase allows stable-telomere maintenance in the absence of Est1, consistent with a role for Est1 in mediating telomerase access. Telomere length homeostasis therefore is maintained in part by restricting access of telomerase to chromosome termini, but this limiting situation can be overcome by directly tethering telomerase to the telomere.</description><subject>Alleles</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cell growth</subject><subject>Cellular senescence</subject><subject>Chromatin. Chromosome</subject><subject>Cyclin B - genetics</subject><subject>Cyclin B - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA binding proteins</subject><subject>DNA, Fungal - metabolism</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Genetic Complementation Test</subject><subject>Genetic mutation</subject><subject>Genetics</subject><subject>Homeostasis</subject><subject>Models, Biological</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Mutation (Biology)</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Proximity</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>RNA</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sprains and strains</subject><subject>Telomerase</subject><subject>Telomerase - genetics</subject><subject>Telomerase - metabolism</subject><subject>Telomere - metabolism</subject><subject>Telomeres</subject><subject>Yeast</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0luL00AUB_AgiltXv8EiQWT1YVPnkrk9dsNaF4p9cPV1mJ2clJQ02Z1JQL-9J6SolaJlHgbO_Ob-T5ILSuaUMvkh-hpaD3Om5VzkXGFVPUlmlBiRGUb402RGCJeZJkqcJS9i3BKCY4Y_T84oEUQKoWcJu4k9TV1bpkXpKU9dTItuB2Xt-i7EtKvSO2iwEFyEdOE9xPgyeVa5JsKrfX-efP14c1d8ylbr5W2xWGVead5nXOel4ryiXEqtqJDAKl6pSnHsuAPiGPE6v3dGMqeV04xA7ikY7TyXxPDz5N207kPoHgeIvd3V0UPTuBa6IVqFlxaS5wrl5b8lUVoZtP-DFA9MpBQI3_wFt90QWryuZZQLbZgY0dWENq4BW7dV1wfnN9DiazVdC1WN5QVuyzSnGnl2hGMrYVf7Y_79gUfSw_d-44YY7e2XzyfT9beT6fXyVKqXqwN6dYz6rmlgAxZzUawPeD5xH7oYA1T2IdQ7F35YSuyYbrtPt8V02zHdWB3_-fX-V4Z7zOgfk6Y4I3i7By5611TBtb6Ov51RjJJxnYuJbSOm_Ncw00YbkvOf8BgEGg</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Evans, Sara K.</creator><creator>Lundblad, Victoria</creator><general>American Society for the Advancement of Science</general><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>0-V</scope><scope>3V.</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88B</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CJNVE</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9-</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0K</scope><scope>M0P</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEDU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Est1 and Cdc13 as Comediators of Telomerase Access</title><author>Evans, Sara K. ; 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Chromosome</topic><topic>Cyclin B - genetics</topic><topic>Cyclin B - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA binding proteins</topic><topic>DNA, Fungal - metabolism</topic><topic>DNA, Single-Stranded - metabolism</topic><topic>Fundamental and applied biological sciences. 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Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant versions of Cdc13 or Est1 confer similar telomere elongation, indicating that close physical proximity can bypass telomerase-defective mutations in either protein. Fusing Cdc13 directly to the catalytic core of telomerase allows stable-telomere maintenance in the absence of Est1, consistent with a role for Est1 in mediating telomerase access. Telomere length homeostasis therefore is maintained in part by restricting access of telomerase to chromosome termini, but this limiting situation can be overcome by directly tethering telomerase to the telomere.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>10506558</pmid><doi>10.1126/science.286.5437.117</doi><tpages>4</tpages></addata></record> |
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| ispartof | Science (American Association for the Advancement of Science), 1999-10, Vol.286 (5437), p.117-120 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | MEDLINE; Science Magazine; JSTOR Archive Collection A-Z Listing |
| subjects | Alleles Binding Sites Biological and medical sciences Cell growth Cellular senescence Chromatin. Chromosome Cyclin B - genetics Cyclin B - metabolism Deoxyribonucleic acid DNA DNA binding proteins DNA, Fungal - metabolism DNA, Single-Stranded - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism Genetic Complementation Test Genetic mutation Genetics Homeostasis Models, Biological Molecular and cellular biology Molecular genetics Mutation Mutation (Biology) Plasmids Proteins Proximity Recombinant Fusion Proteins - metabolism RNA Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins Sprains and strains Telomerase Telomerase - genetics Telomerase - metabolism Telomere - metabolism Telomeres Yeast |
| title | Est1 and Cdc13 as Comediators of Telomerase Access |
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