Structure of a Transcribing T7 RNA Polymerase Initiation Complex

The structure of a T7 RNA polymerase (T7 RNAP) initiation complex captured transcribing a trinucleotide of RNA from a 17-base pair promoter DNA containing a 5-nucleotide single-strand template extension was determined at a resolution of 2.4 angstroms. Binding of the upstream duplex portion of the pr...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1999-12, Vol.286 (5448), p.2305-2309
Hauptverfasser:	Graham M. T. Cheetham, Steitz, Thomas A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Active sites Amino Acid Motifs Bacteriophage T7 - enzymology BASIC BIOLOGICAL SCIENCES Binding sites Biological and medical sciences Catalytic Domain COMPLEXES Conserved Sequence Crystallography, X-Ray Crystals Deoxyribonucleic acid DNA DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism DNA-Directed DNA Polymerase - chemistry DNA-Directed DNA Polymerase - metabolism DNA-Directed RNA Polymerases - chemistry DNA-Directed RNA Polymerases - metabolism Enzymes Fundamental and applied biological sciences. Psychology Hydrogen Bonding Hydrogen bonds Models, Molecular Molecular and cellular biology Molecular genetics N-Acetylmuramoyl-L-alanine Amidase - metabolism NATIONAL SYNCHROTRON LIGHT SOURCE NSLS Nucleic Acid Conformation Nucleic Acid Heteroduplexes - chemistry Nucleic Acid Heteroduplexes - metabolism Nucleotides Oligoribonucleotides - chemistry Oligoribonucleotides - metabolism Polymerase chain reaction Polymers Promoter Regions, Genetic Protein Conformation Protein Structure, Tertiary Research Article Ribonucleic acid Ribonucleotides RNA RNA POLYMERASES RNA, Messenger - biosynthesis RNA, Messenger - chemistry RNA, Messenger - genetics Substrate Specificity Templates, Genetic Thumb Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transcripts (Written Records) Viral Proteins
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