Effect of hydrogen ion concentration on rhodopsin-lipid interactions
This study concerns the role titratable chemical groups play in rhodopsin-lipid interactions. Tempo partitioning techniques are used to construct DMPC-rhodopsin partial binary phase diagrams. Such diagrams are constructed at pH 7.0, where rhodopsin has a net negative charge, and at pH 5.0, where rho...
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| Veröffentlicht in: | Biochemistry (Easton) 1982-09, Vol.21 (20), p.5101-5105 |
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| container_title | Biochemistry (Easton) |
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| creator | Fischer, Thomas H Williams, Theodore P |
| description | This study concerns the role titratable chemical groups play in rhodopsin-lipid interactions. Tempo partitioning techniques are used to construct DMPC-rhodopsin partial binary phase diagrams. Such diagrams are constructed at pH 7.0, where rhodopsin has a net negative charge, and at pH 5.0, where rhodopsin is virtually isoelectric. It is found that at pH 5.0, increases in rhodopsin content induce a depression in temperature of the solidus curve. At pH 7.0, the solidus curve is not a strong function of temperature. Additional studies, involving nitroxide-labeled palmitic acid, methyl palmitate, and lecithins, indicate that the pH-dependent changes in the phase behavior of DMPC-rhodopsin systems are due to the titration of rhodopsin's carboxylic acid groups. |
| doi_str_mv | 10.1021/bi00263a039 |
| format | Article |
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Tempo partitioning techniques are used to construct DMPC-rhodopsin partial binary phase diagrams. Such diagrams are constructed at pH 7.0, where rhodopsin has a net negative charge, and at pH 5.0, where rhodopsin is virtually isoelectric. It is found that at pH 5.0, increases in rhodopsin content induce a depression in temperature of the solidus curve. At pH 7.0, the solidus curve is not a strong function of temperature. Additional studies, involving nitroxide-labeled palmitic acid, methyl palmitate, and lecithins, indicate that the pH-dependent changes in the phase behavior of DMPC-rhodopsin systems are due to the titration of rhodopsin's carboxylic acid groups.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00263a039</identifier><identifier>PMID: 6291601</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Chemical Phenomena ; Chemistry, Physical ; Cyclic N-Oxides ; Dimyristoylphosphatidylcholine ; Electron Spin Resonance Spectroscopy ; Hydrogen-Ion Concentration ; Membranes, Artificial ; Phosphatidylcholines ; Retinal Pigments ; Rhodopsin ; Spin Labels ; Temperature</subject><ispartof>Biochemistry (Easton), 1982-09, Vol.21 (20), p.5101-5105</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a385t-af76a81ac70d7dc487baedf93c098e51222d29b21e59142fb50d8f6683614adc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00263a039$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00263a039$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6291601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fischer, Thomas H</creatorcontrib><creatorcontrib>Williams, Theodore P</creatorcontrib><title>Effect of hydrogen ion concentration on rhodopsin-lipid interactions</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>This study concerns the role titratable chemical groups play in rhodopsin-lipid interactions. Tempo partitioning techniques are used to construct DMPC-rhodopsin partial binary phase diagrams. Such diagrams are constructed at pH 7.0, where rhodopsin has a net negative charge, and at pH 5.0, where rhodopsin is virtually isoelectric. It is found that at pH 5.0, increases in rhodopsin content induce a depression in temperature of the solidus curve. At pH 7.0, the solidus curve is not a strong function of temperature. Additional studies, involving nitroxide-labeled palmitic acid, methyl palmitate, and lecithins, indicate that the pH-dependent changes in the phase behavior of DMPC-rhodopsin systems are due to the titration of rhodopsin's carboxylic acid groups.</description><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Cyclic N-Oxides</subject><subject>Dimyristoylphosphatidylcholine</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Hydrogen-Ion Concentration</subject><subject>Membranes, Artificial</subject><subject>Phosphatidylcholines</subject><subject>Retinal Pigments</subject><subject>Rhodopsin</subject><subject>Spin Labels</subject><subject>Temperature</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMoWh8r18KsdCGjN5k8JkutVkVBQV2HTB42tZ3UZAr6753SIi4E4cLlcD7OvRyEDjGcYSD4vAkAhFcaKrmBBpgRKKmUbBMNAICXRHLYQbs5T3pJQdBttM2JxBzwAF1de-9MV0RfjL9sim-uLUJsCxNb49ou6W6p-knjaOM8h7achnmwRWg7l7RZ2nkfbXk9ze5gvffQ6-j6ZXhbPjze3A0vHkpd1awrtRdc11gbAVZYQ2vRaGe9rAzI2jFMCLFENgQ7JjElvmFga895XXFMtTXVHjpe5c5T_Fi43KlZyMZNp7p1cZGVoJUAqMm_IGYMOEjag6cr0KSYc3JezVOY6fSlMKhluepXuT19tI5dNDNnf9h1m71frvyQO_f5Y-v0rrioBFMvT8_qavR8Sfsn1X3Pn6x4bbKaxEVq-_b-vPwNjDWPhA</recordid><startdate>19820928</startdate><enddate>19820928</enddate><creator>Fischer, Thomas H</creator><creator>Williams, Theodore P</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19820928</creationdate><title>Effect of hydrogen ion concentration on rhodopsin-lipid interactions</title><author>Fischer, Thomas H ; Williams, Theodore P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a385t-af76a81ac70d7dc487baedf93c098e51222d29b21e59142fb50d8f6683614adc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Cyclic N-Oxides</topic><topic>Dimyristoylphosphatidylcholine</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Hydrogen-Ion Concentration</topic><topic>Membranes, Artificial</topic><topic>Phosphatidylcholines</topic><topic>Retinal Pigments</topic><topic>Rhodopsin</topic><topic>Spin Labels</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fischer, Thomas H</creatorcontrib><creatorcontrib>Williams, Theodore P</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fischer, Thomas H</au><au>Williams, Theodore P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of hydrogen ion concentration on rhodopsin-lipid interactions</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1982-09-28</date><risdate>1982</risdate><volume>21</volume><issue>20</issue><spage>5101</spage><epage>5105</epage><pages>5101-5105</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>This study concerns the role titratable chemical groups play in rhodopsin-lipid interactions. 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| ispartof | Biochemistry (Easton), 1982-09, Vol.21 (20), p.5101-5105 |
| issn | 0006-2960 1520-4995 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Chemical Phenomena Chemistry, Physical Cyclic N-Oxides Dimyristoylphosphatidylcholine Electron Spin Resonance Spectroscopy Hydrogen-Ion Concentration Membranes, Artificial Phosphatidylcholines Retinal Pigments Rhodopsin Spin Labels Temperature |
| title | Effect of hydrogen ion concentration on rhodopsin-lipid interactions |
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