Effects of Chronic Red Wine Consumption on the Expression of Vascular Endothelial Growth Factor, Angiopoietin 1, Angiopoietin 2, and Its Receptors in Rat Erectile Tissue
Long‐term consumption of red wine (RW) apparently confers some protection against cardiovascular diseases due to antiatherosclerotic properties of polyphenols and ethanol (EtOH). There is some evidence indicating that they do so by regulating angiogenesis, but the mechanism and the modulator factors...
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| Veröffentlicht in: | Journal of food science 2010-04, Vol.75 (3), p.H79-H86 |
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| creator | Neves, Delminda R.G.L.M. Tomada, Inês M.A.S.C.M. Assunção, Marco M.B. Marques, Franklim A.P. Almeida, Henrique M.N. Andrade, José P.A.V. |
| description | Long‐term consumption of red wine (RW) apparently confers some protection against cardiovascular diseases due to antiatherosclerotic properties of polyphenols and ethanol (EtOH). There is some evidence indicating that they do so by regulating angiogenesis, but the mechanism and the modulator factors involved are largely unknown. The aim of this study was to evaluate the effects of chronic ingestion of RW in vascular structure and in the pattern of expression of vascular growth factors in the rat corpus cavernosum. Male Wistar rats aged 6 mo were treated with RW or an equivalent EtOH solution, as the only liquid source for 6 mo. Expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and angiopoietin 2, and their receptors (VEGFR1, VEGFR2, and Tie2) in cavernous tissue was assayed by immunofluorescence and Western blotting. A reduction of VEGF and VEGFR2 expression, respectively, in smooth muscle and endothelial cells was observed in RW‐treated animals, which was balanced by an increase in angiopoietins/Tie2 expression. In EtOH rats, only a decrease in expression of the receptors VEGFR2 and Tie2 was observed. These results, taken together, suggest that antioxidants present in RW activate selected mechanisms for the maintenance of cavernous tissue vascularization. However, functional studies will be necessary to elucidate if RW is of benefit in the prevention of deleterious vascular events associated with ED. |
| doi_str_mv | 10.1111/j.1750-3841.2010.01527.x |
| format | Article |
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There is some evidence indicating that they do so by regulating angiogenesis, but the mechanism and the modulator factors involved are largely unknown. The aim of this study was to evaluate the effects of chronic ingestion of RW in vascular structure and in the pattern of expression of vascular growth factors in the rat corpus cavernosum. Male Wistar rats aged 6 mo were treated with RW or an equivalent EtOH solution, as the only liquid source for 6 mo. Expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and angiopoietin 2, and their receptors (VEGFR1, VEGFR2, and Tie2) in cavernous tissue was assayed by immunofluorescence and Western blotting. A reduction of VEGF and VEGFR2 expression, respectively, in smooth muscle and endothelial cells was observed in RW‐treated animals, which was balanced by an increase in angiopoietins/Tie2 expression. In EtOH rats, only a decrease in expression of the receptors VEGFR2 and Tie2 was observed. These results, taken together, suggest that antioxidants present in RW activate selected mechanisms for the maintenance of cavernous tissue vascularization. However, functional studies will be necessary to elucidate if RW is of benefit in the prevention of deleterious vascular events associated with ED.</description><identifier>ISSN: 0022-1147</identifier><identifier>EISSN: 1750-3841</identifier><identifier>DOI: 10.1111/j.1750-3841.2010.01527.x</identifier><identifier>PMID: 20492297</identifier><identifier>CODEN: JFDSAZ</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>alcohol ; Alcohol Drinking - metabolism ; Angiopoietin-1 - metabolism ; Angiopoietin-2 - metabolism ; angiopoietins 1 and 2 ; Animals ; Antioxidants ; Biological and medical sciences ; Cardiovascular disease ; corpus cavernosum ; Down-Regulation ; Effects ; Ethanol ; Fermented food industries ; Fluorescent Antibody Technique, Indirect ; Food industries ; Food science ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Impotence, Vasculogenic - prevention & control ; Male ; Penis - blood supply ; Penis - metabolism ; Penis - pathology ; Random Allocation ; Rats ; Rats, Wistar ; Receptor, TIE-2 - metabolism ; Receptors, Cell Surface - metabolism ; red wine ; Rodents ; Tissues ; Up-Regulation ; Vascular Endothelial Growth Factor A - metabolism ; Vascular Endothelial Growth Factor Receptor-1 - metabolism ; Vascular Endothelial Growth Factor Receptor-2 - metabolism ; VEGF ; Wine ; Wines ; Wines and vinegars</subject><ispartof>Journal of food science, 2010-04, Vol.75 (3), p.H79-H86</ispartof><rights>2010 Institute of Food Technologists</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Institute of Food Technologists Apr 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4967-b2203897da69a30b10786cba6f4eebcdbbba73470b696131c113e0b0cbaea4b93</citedby><cites>FETCH-LOGICAL-c4967-b2203897da69a30b10786cba6f4eebcdbbba73470b696131c113e0b0cbaea4b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1750-3841.2010.01527.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1750-3841.2010.01527.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22643229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20492297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neves, Delminda R.G.L.M.</creatorcontrib><creatorcontrib>Tomada, Inês M.A.S.C.M.</creatorcontrib><creatorcontrib>Assunção, Marco M.B.</creatorcontrib><creatorcontrib>Marques, Franklim A.P.</creatorcontrib><creatorcontrib>Almeida, Henrique M.N.</creatorcontrib><creatorcontrib>Andrade, José P.A.V.</creatorcontrib><title>Effects of Chronic Red Wine Consumption on the Expression of Vascular Endothelial Growth Factor, Angiopoietin 1, Angiopoietin 2, and Its Receptors in Rat Erectile Tissue</title><title>Journal of food science</title><addtitle>J Food Sci</addtitle><description>Long‐term consumption of red wine (RW) apparently confers some protection against cardiovascular diseases due to antiatherosclerotic properties of polyphenols and ethanol (EtOH). There is some evidence indicating that they do so by regulating angiogenesis, but the mechanism and the modulator factors involved are largely unknown. The aim of this study was to evaluate the effects of chronic ingestion of RW in vascular structure and in the pattern of expression of vascular growth factors in the rat corpus cavernosum. Male Wistar rats aged 6 mo were treated with RW or an equivalent EtOH solution, as the only liquid source for 6 mo. Expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and angiopoietin 2, and their receptors (VEGFR1, VEGFR2, and Tie2) in cavernous tissue was assayed by immunofluorescence and Western blotting. A reduction of VEGF and VEGFR2 expression, respectively, in smooth muscle and endothelial cells was observed in RW‐treated animals, which was balanced by an increase in angiopoietins/Tie2 expression. In EtOH rats, only a decrease in expression of the receptors VEGFR2 and Tie2 was observed. These results, taken together, suggest that antioxidants present in RW activate selected mechanisms for the maintenance of cavernous tissue vascularization. However, functional studies will be necessary to elucidate if RW is of benefit in the prevention of deleterious vascular events associated with ED.</description><subject>alcohol</subject><subject>Alcohol Drinking - metabolism</subject><subject>Angiopoietin-1 - metabolism</subject><subject>Angiopoietin-2 - metabolism</subject><subject>angiopoietins 1 and 2</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Biological and medical sciences</subject><subject>Cardiovascular disease</subject><subject>corpus cavernosum</subject><subject>Down-Regulation</subject><subject>Effects</subject><subject>Ethanol</subject><subject>Fermented food industries</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Food industries</subject><subject>Food science</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Impotence, Vasculogenic - prevention & control</subject><subject>Male</subject><subject>Penis - blood supply</subject><subject>Penis - metabolism</subject><subject>Penis - pathology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor, TIE-2 - metabolism</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>red wine</subject><subject>Rodents</subject><subject>Tissues</subject><subject>Up-Regulation</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vascular Endothelial Growth Factor Receptor-1 - metabolism</subject><subject>Vascular Endothelial Growth Factor Receptor-2 - metabolism</subject><subject>VEGF</subject><subject>Wine</subject><subject>Wines</subject><subject>Wines and vinegars</subject><issn>0022-1147</issn><issn>1750-3841</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd9u0zAUxiMEYmXwCshCQrtZOv-rXd8gTaEtQxOgMuil5TgOdUntYCda90i8Jc7aFWk3YFmyzzm_8-nYX5YBBMcorYvNGPEJzMmUojGGKQvRBPPx7kk2OhaeZiMIMc4RovwkexHjBg4xYc-zEwypwFjwUfZ7VtdGdxH4GhTr4J3VYGkqsLLOgMK72G_bznoH0u7WBsx2bTAx3mdq8F1F3TcqgJmrfCo3VjVgEfxttwZzpTsfzsGl-2F9663prAPoUYzPgXIVuEoDLI02beqIIOWXqgOzkAazjQE3NsbevMye1aqJ5tXhPM2-zWc3xYf8-vPiqri8zjUVjOclxpBMBa8UE4rAEkE-ZbpUrKbGlLoqy1JxQjksmWCIII0QMbCECTGKloKcZmd73Tb4X72JndzaqE3TKGd8HyWnhHGGBPo3SchkIggZNN88Ije-Dy49QyJBKZkijhM03UM6-BiDqWUb7FaFO4mgHGyXGzm4Kwd35WC7vLdd7lLr64N-X25NdWx88DkBbw9Ackw1dVBO2_iXw4ySBCbu3Z67TR9_998DyI_z91-HaxLI9wI2dmZ3FFDhp2Sc8IlcfVrIZSFWhfgi5IL8AUcv2CA</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Neves, Delminda R.G.L.M.</creator><creator>Tomada, Inês M.A.S.C.M.</creator><creator>Assunção, Marco M.B.</creator><creator>Marques, Franklim A.P.</creator><creator>Almeida, Henrique M.N.</creator><creator>Andrade, José P.A.V.</creator><general>Blackwell Publishing Inc</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201004</creationdate><title>Effects of Chronic Red Wine Consumption on the Expression of Vascular Endothelial Growth Factor, Angiopoietin 1, Angiopoietin 2, and Its Receptors in Rat Erectile Tissue</title><author>Neves, Delminda R.G.L.M. ; Tomada, Inês M.A.S.C.M. ; Assunção, Marco M.B. ; Marques, Franklim A.P. ; Almeida, Henrique M.N. ; Andrade, José P.A.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4967-b2203897da69a30b10786cba6f4eebcdbbba73470b696131c113e0b0cbaea4b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>alcohol</topic><topic>Alcohol Drinking - metabolism</topic><topic>Angiopoietin-1 - metabolism</topic><topic>Angiopoietin-2 - metabolism</topic><topic>angiopoietins 1 and 2</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Biological and medical sciences</topic><topic>Cardiovascular disease</topic><topic>corpus cavernosum</topic><topic>Down-Regulation</topic><topic>Effects</topic><topic>Ethanol</topic><topic>Fermented food industries</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Food industries</topic><topic>Food science</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Impotence, Vasculogenic - prevention & control</topic><topic>Male</topic><topic>Penis - blood supply</topic><topic>Penis - metabolism</topic><topic>Penis - pathology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptor, TIE-2 - metabolism</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>red wine</topic><topic>Rodents</topic><topic>Tissues</topic><topic>Up-Regulation</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Vascular Endothelial Growth Factor Receptor-1 - metabolism</topic><topic>Vascular Endothelial Growth Factor Receptor-2 - metabolism</topic><topic>VEGF</topic><topic>Wine</topic><topic>Wines</topic><topic>Wines and vinegars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neves, Delminda R.G.L.M.</creatorcontrib><creatorcontrib>Tomada, Inês M.A.S.C.M.</creatorcontrib><creatorcontrib>Assunção, Marco M.B.</creatorcontrib><creatorcontrib>Marques, Franklim A.P.</creatorcontrib><creatorcontrib>Almeida, Henrique M.N.</creatorcontrib><creatorcontrib>Andrade, José P.A.V.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neves, Delminda R.G.L.M.</au><au>Tomada, Inês M.A.S.C.M.</au><au>Assunção, Marco M.B.</au><au>Marques, Franklim A.P.</au><au>Almeida, Henrique M.N.</au><au>Andrade, José P.A.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Chronic Red Wine Consumption on the Expression of Vascular Endothelial Growth Factor, Angiopoietin 1, Angiopoietin 2, and Its Receptors in Rat Erectile Tissue</atitle><jtitle>Journal of food science</jtitle><addtitle>J Food Sci</addtitle><date>2010-04</date><risdate>2010</risdate><volume>75</volume><issue>3</issue><spage>H79</spage><epage>H86</epage><pages>H79-H86</pages><issn>0022-1147</issn><eissn>1750-3841</eissn><coden>JFDSAZ</coden><abstract>Long‐term consumption of red wine (RW) apparently confers some protection against cardiovascular diseases due to antiatherosclerotic properties of polyphenols and ethanol (EtOH). There is some evidence indicating that they do so by regulating angiogenesis, but the mechanism and the modulator factors involved are largely unknown. The aim of this study was to evaluate the effects of chronic ingestion of RW in vascular structure and in the pattern of expression of vascular growth factors in the rat corpus cavernosum. Male Wistar rats aged 6 mo were treated with RW or an equivalent EtOH solution, as the only liquid source for 6 mo. Expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and angiopoietin 2, and their receptors (VEGFR1, VEGFR2, and Tie2) in cavernous tissue was assayed by immunofluorescence and Western blotting. A reduction of VEGF and VEGFR2 expression, respectively, in smooth muscle and endothelial cells was observed in RW‐treated animals, which was balanced by an increase in angiopoietins/Tie2 expression. In EtOH rats, only a decrease in expression of the receptors VEGFR2 and Tie2 was observed. These results, taken together, suggest that antioxidants present in RW activate selected mechanisms for the maintenance of cavernous tissue vascularization. However, functional studies will be necessary to elucidate if RW is of benefit in the prevention of deleterious vascular events associated with ED.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>20492297</pmid><doi>10.1111/j.1750-3841.2010.01527.x</doi><tpages>8</tpages></addata></record> |
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| subjects | alcohol Alcohol Drinking - metabolism Angiopoietin-1 - metabolism Angiopoietin-2 - metabolism angiopoietins 1 and 2 Animals Antioxidants Biological and medical sciences Cardiovascular disease corpus cavernosum Down-Regulation Effects Ethanol Fermented food industries Fluorescent Antibody Technique, Indirect Food industries Food science Fundamental and applied biological sciences. Psychology Immunohistochemistry Impotence, Vasculogenic - prevention & control Male Penis - blood supply Penis - metabolism Penis - pathology Random Allocation Rats Rats, Wistar Receptor, TIE-2 - metabolism Receptors, Cell Surface - metabolism red wine Rodents Tissues Up-Regulation Vascular Endothelial Growth Factor A - metabolism Vascular Endothelial Growth Factor Receptor-1 - metabolism Vascular Endothelial Growth Factor Receptor-2 - metabolism VEGF Wine Wines Wines and vinegars |
| title | Effects of Chronic Red Wine Consumption on the Expression of Vascular Endothelial Growth Factor, Angiopoietin 1, Angiopoietin 2, and Its Receptors in Rat Erectile Tissue |
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