Functional Human Corneal Equivalents Constructed from Cell Lines
Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological si...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1999-12, Vol.286 (5447), p.2169-2172 |
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| creator | Griffith, May Osborne, Rosemarie Munger, Rejean Xiong, Xiaojuan Doillon, Charles J. Noelani L. C. Laycock Hakim, Malik Song, Ying Watsky, Mitchell A. |
| description | Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues. |
| doi_str_mv | 10.1126/science.286.5447.2169 |
| format | Article |
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C. Laycock ; Hakim, Malik ; Song, Ying ; Watsky, Mitchell A.</creator><creatorcontrib>Griffith, May ; Osborne, Rosemarie ; Munger, Rejean ; Xiong, Xiaojuan ; Doillon, Charles J. ; Noelani L. C. Laycock ; Hakim, Malik ; Song, Ying ; Watsky, Mitchell A.</creatorcontrib><description>Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.286.5447.2169</identifier><identifier>PMID: 10591651</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Aneuploidy ; Animal Testing Alternatives ; Biological and medical sciences ; Biomedical Engineering ; Cell Line ; Cell lines ; Cells ; Cells, Cultured ; Chemical hazards ; Chondroitin Sulfates ; Collagen ; Cornea ; Cornea - cytology ; Cornea - growth & development ; Cornea - physiology ; Corneal Opacity - chemically induced ; Corneal Stroma - cytology ; Corneal Stroma - growth & development ; Corneal Stroma - physiology ; Corneal Transplantation ; Cross-Linking Reagents ; Culture Techniques ; Cultured cells ; Electric current ; Electrophysiology ; Endothelium, Corneal - cytology ; Endothelium, Corneal - growth & development ; Epithelial cells ; Epithelium, Corneal - cytology ; Epithelium, Corneal - growth & development ; Eye banks ; Eyes ; Eyes & eyesight ; Gene Expression ; Glutaral ; Humans ; Individualized Instruction ; Ion Channels ; Medical sciences ; Messenger RNA ; Ouabain - pharmacology ; Patch-Clamp Techniques ; Physiology ; Sodium Dodecyl Sulfate - pharmacology ; Studies ; Surgery (general aspects). 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Graft diseases ; Surgery of the eye and orbit</subject><ispartof>Science (American Association for the Advancement of Science), 1999-12, Vol.286 (5447), p.2169-2172</ispartof><rights>Copyright 1999 American Association for the Advancement of Science</rights><rights>2000 INIST-CNRS</rights><rights>COPYRIGHT 1999 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1999 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Dec 10, 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c786t-2d5f381693886c99d909bc4b4dc0ea088575024583e0d3cb8f0a073d49ce35e53</citedby><cites>FETCH-LOGICAL-c786t-2d5f381693886c99d909bc4b4dc0ea088575024583e0d3cb8f0a073d49ce35e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2899997$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2899997$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1264037$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10591651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Griffith, May</creatorcontrib><creatorcontrib>Osborne, Rosemarie</creatorcontrib><creatorcontrib>Munger, Rejean</creatorcontrib><creatorcontrib>Xiong, Xiaojuan</creatorcontrib><creatorcontrib>Doillon, Charles J.</creatorcontrib><creatorcontrib>Noelani L. C. Laycock</creatorcontrib><creatorcontrib>Hakim, Malik</creatorcontrib><creatorcontrib>Song, Ying</creatorcontrib><creatorcontrib>Watsky, Mitchell A.</creatorcontrib><title>Functional Human Corneal Equivalents Constructed from Cell Lines</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.</description><subject>Aneuploidy</subject><subject>Animal Testing Alternatives</subject><subject>Biological and medical sciences</subject><subject>Biomedical Engineering</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Chemical hazards</subject><subject>Chondroitin Sulfates</subject><subject>Collagen</subject><subject>Cornea</subject><subject>Cornea - cytology</subject><subject>Cornea - growth & development</subject><subject>Cornea - physiology</subject><subject>Corneal Opacity - chemically induced</subject><subject>Corneal Stroma - cytology</subject><subject>Corneal Stroma - growth & development</subject><subject>Corneal Stroma - physiology</subject><subject>Corneal Transplantation</subject><subject>Cross-Linking Reagents</subject><subject>Culture Techniques</subject><subject>Cultured cells</subject><subject>Electric current</subject><subject>Electrophysiology</subject><subject>Endothelium, Corneal - cytology</subject><subject>Endothelium, Corneal - growth & development</subject><subject>Epithelial cells</subject><subject>Epithelium, Corneal - cytology</subject><subject>Epithelium, Corneal - growth & development</subject><subject>Eye banks</subject><subject>Eyes</subject><subject>Eyes & eyesight</subject><subject>Gene Expression</subject><subject>Glutaral</subject><subject>Humans</subject><subject>Individualized Instruction</subject><subject>Ion Channels</subject><subject>Medical sciences</subject><subject>Messenger RNA</subject><subject>Ouabain - pharmacology</subject><subject>Patch-Clamp Techniques</subject><subject>Physiology</subject><subject>Sodium Dodecyl Sulfate - pharmacology</subject><subject>Studies</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>10591651</pmid><doi>10.1126/science.286.5447.2169</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1999-12, Vol.286 (5447), p.2169-2172 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743669465 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Aneuploidy Animal Testing Alternatives Biological and medical sciences Biomedical Engineering Cell Line Cell lines Cells Cells, Cultured Chemical hazards Chondroitin Sulfates Collagen Cornea Cornea - cytology Cornea - growth & development Cornea - physiology Corneal Opacity - chemically induced Corneal Stroma - cytology Corneal Stroma - growth & development Corneal Stroma - physiology Corneal Transplantation Cross-Linking Reagents Culture Techniques Cultured cells Electric current Electrophysiology Endothelium, Corneal - cytology Endothelium, Corneal - growth & development Epithelial cells Epithelium, Corneal - cytology Epithelium, Corneal - growth & development Eye banks Eyes Eyes & eyesight Gene Expression Glutaral Humans Individualized Instruction Ion Channels Medical sciences Messenger RNA Ouabain - pharmacology Patch-Clamp Techniques Physiology Sodium Dodecyl Sulfate - pharmacology Studies Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the eye and orbit |
| title | Functional Human Corneal Equivalents Constructed from Cell Lines |
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