Semiconductor Nanocrystals as Fluorescent Biological Labels
Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitat...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1998-09, Vol.281 (5385), p.2013-2016 |
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| creator | Bruchez, Marcel Moronne, Mario Gin, Peter Weiss, Shimon Alivisatos, A. Paul |
| description | Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores. |
| doi_str_mv | 10.1126/science.281.5385.2013 |
| format | Article |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | 3T3 Cells Actins Animals Biological and medical sciences Biology Biotinylation Cadmium Compounds Cell Nucleus - ultrastructure Chemistry Crystallization Cytometry Cytoskeleton - ultrastructure Diverse techniques Dyes Emission spectra Fluorescence Fluorescent Dyes Fundamental and applied biological sciences. Psychology Materials science Mice Microscopy, Confocal Microscopy, Fluorescence Molecular and cellular biology Molecular Probe Techniques Molecules Nanocrystals Organic Chemistry Rodents Scientific Concepts Selenium Compounds Semiconductors Silicon Dioxide Solubility Spectrometry, Fluorescence Staining and Labeling - methods Sulfides Water Wavelengths |
| title | Semiconductor Nanocrystals as Fluorescent Biological Labels |
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