Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays
Suspension arrays for protein-based assays have been developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles to overcome the problems with current systems which use color-coded rigid microparticles as protein supports. Various shapes of hydrogel microparticles were fabricated...
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| Veröffentlicht in: | Biomedical microdevices 2008-12, Vol.10 (6), p.813-822 |
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| creator | Lee, Woojin Choi, Dongkil Kim, Jung-Hyun Koh, Won-Gun |
| description | Suspension arrays for protein-based assays have been developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles to overcome the problems with current systems which use color-coded rigid microparticles as protein supports. Various shapes of hydrogel microparticles were fabricated by a two-step process consisting of photopatterning and flushing using a poly(dimethylsiloxane) (PDMS) channel as a molding insert. Hydrogel microparticles with lateral dimensions ranging from 50 to 300 μm were fabricated using different molecular weights of PEG (700, 3,400, and 8,000 Da), by which the water content and swelling behavior of the hydrogel microparticles could be controlled. Protein-entrapped hydrogel microparticles were prepared in a suspension array format, and PEG hydrogel could encapsulate proteins without deactivation for a week due to its high water content and soft nature. The sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) was successfully investigated using fluorescence detection, demonstrating one possible application of suspension arrays. Furthermore, a mixture of two different shapes of hydrogel microparticles containing GOX/POD and alkaline phosphatase (AP), respectively, was prepared and the shape-coded suspension array was used for simultaneous characterization of two different enzyme-catalyzed reactions. |
| doi_str_mv | 10.1007/s10544-008-9196-1 |
| format | Article |
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Various shapes of hydrogel microparticles were fabricated by a two-step process consisting of photopatterning and flushing using a poly(dimethylsiloxane) (PDMS) channel as a molding insert. Hydrogel microparticles with lateral dimensions ranging from 50 to 300 μm were fabricated using different molecular weights of PEG (700, 3,400, and 8,000 Da), by which the water content and swelling behavior of the hydrogel microparticles could be controlled. Protein-entrapped hydrogel microparticles were prepared in a suspension array format, and PEG hydrogel could encapsulate proteins without deactivation for a week due to its high water content and soft nature. The sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) was successfully investigated using fluorescence detection, demonstrating one possible application of suspension arrays. Furthermore, a mixture of two different shapes of hydrogel microparticles containing GOX/POD and alkaline phosphatase (AP), respectively, was prepared and the shape-coded suspension array was used for simultaneous characterization of two different enzyme-catalyzed reactions.</description><identifier>ISSN: 1387-2176</identifier><identifier>EISSN: 1572-8781</identifier><identifier>DOI: 10.1007/s10544-008-9196-1</identifier><identifier>PMID: 18561028</identifier><identifier>CODEN: BMICFC</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Alkaline Phosphatase - chemistry ; Bioassays ; Biological and Medical Physics ; Biological Assay - instrumentation ; Biological Assay - methods ; Biomedical Engineering and Bioengineering ; Biomedical research ; Biophysics ; Dimethylpolysiloxanes - chemistry ; Engineering ; Engineering Fluid Dynamics ; Enzymes ; Enzymes, Immobilized - chemistry ; Fluorescence ; Glucose Oxidase - chemistry ; Hydrogels - chemistry ; Nanoparticles ; Nanotechnology ; Particle Size ; Peroxidase - chemistry ; Polyethylene Glycols - chemistry ; Scientific method ; Suspensions</subject><ispartof>Biomedical microdevices, 2008-12, Vol.10 (6), p.813-822</ispartof><rights>Springer Science+Business Media, LLC 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-5281b629f8c961a2089b3ecd9ddc987875a5dca6a0b380b13934ac6faf0a329f3</citedby><cites>FETCH-LOGICAL-c463t-5281b629f8c961a2089b3ecd9ddc987875a5dca6a0b380b13934ac6faf0a329f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10544-008-9196-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10544-008-9196-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18561028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Woojin</creatorcontrib><creatorcontrib>Choi, Dongkil</creatorcontrib><creatorcontrib>Kim, Jung-Hyun</creatorcontrib><creatorcontrib>Koh, Won-Gun</creatorcontrib><title>Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays</title><title>Biomedical microdevices</title><addtitle>Biomed Microdevices</addtitle><addtitle>Biomed Microdevices</addtitle><description>Suspension arrays for protein-based assays have been developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles to overcome the problems with current systems which use color-coded rigid microparticles as protein supports. Various shapes of hydrogel microparticles were fabricated by a two-step process consisting of photopatterning and flushing using a poly(dimethylsiloxane) (PDMS) channel as a molding insert. Hydrogel microparticles with lateral dimensions ranging from 50 to 300 μm were fabricated using different molecular weights of PEG (700, 3,400, and 8,000 Da), by which the water content and swelling behavior of the hydrogel microparticles could be controlled. Protein-entrapped hydrogel microparticles were prepared in a suspension array format, and PEG hydrogel could encapsulate proteins without deactivation for a week due to its high water content and soft nature. The sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) was successfully investigated using fluorescence detection, demonstrating one possible application of suspension arrays. Furthermore, a mixture of two different shapes of hydrogel microparticles containing GOX/POD and alkaline phosphatase (AP), respectively, was prepared and the shape-coded suspension array was used for simultaneous characterization of two different enzyme-catalyzed reactions.</description><subject>Alkaline Phosphatase - chemistry</subject><subject>Bioassays</subject><subject>Biological and Medical Physics</subject><subject>Biological Assay - instrumentation</subject><subject>Biological Assay - methods</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical research</subject><subject>Biophysics</subject><subject>Dimethylpolysiloxanes - chemistry</subject><subject>Engineering</subject><subject>Engineering Fluid Dynamics</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Fluorescence</subject><subject>Glucose Oxidase - chemistry</subject><subject>Hydrogels - chemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Particle Size</subject><subject>Peroxidase - chemistry</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Scientific method</subject><subject>Suspensions</subject><issn>1387-2176</issn><issn>1572-8781</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkkGPFCEQhYnRuLujP8CLIR70hFLQ0HA0G3VNNvGgnglN07NsupsWuhPn31uTmWQTE90TkPreA6oeIa-AvwfO2w8VuGoaxrlhFqxm8IRcgmoFM62Bp7iXpmUCWn1Brmq95xwhrZ-TCzBKAxfmkizft7rEuaY8U1-KP1SaB3p36Evex5FOKZS8-LKmMMZKlxLxEHvaHehyl1csrWssc5r3dMiFTtu4pmWMvxFZSl5jmlnn61GQsq8V7V-QZ4Mfa3x5Xnfk5-dPP65v2O23L1-vP96y0Gi5MiUMdFrYwQSrwQtubCdj6G3fB4vfa5VXffDa804a3oG0svFBD37gXqJM7si7ky--49cW6-qmVEMcRz_HvFXXNlIL1aJuR97-l9QWm4xtfRQUHKcASj4KglXcqFYh-OYv8D5vZca-OAECGqN4gxCcIBxFrSUObilp8uXggLtjDtwpBw5z4I45cICa12fjrZti_6A4Dx4BcQIqluZ9LA83_9v1D7dbv14</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Lee, Woojin</creator><creator>Choi, Dongkil</creator><creator>Kim, Jung-Hyun</creator><creator>Koh, Won-Gun</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7TB</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays</title><author>Lee, Woojin ; Choi, Dongkil ; Kim, Jung-Hyun ; Koh, Won-Gun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-5281b629f8c961a2089b3ecd9ddc987875a5dca6a0b380b13934ac6faf0a329f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alkaline Phosphatase - chemistry</topic><topic>Bioassays</topic><topic>Biological and Medical Physics</topic><topic>Biological Assay - instrumentation</topic><topic>Biological Assay - methods</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical research</topic><topic>Biophysics</topic><topic>Dimethylpolysiloxanes - chemistry</topic><topic>Engineering</topic><topic>Engineering Fluid Dynamics</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Fluorescence</topic><topic>Glucose Oxidase - chemistry</topic><topic>Hydrogels - chemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Particle Size</topic><topic>Peroxidase - chemistry</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Scientific method</topic><topic>Suspensions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Woojin</creatorcontrib><creatorcontrib>Choi, Dongkil</creatorcontrib><creatorcontrib>Kim, Jung-Hyun</creatorcontrib><creatorcontrib>Koh, Won-Gun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical microdevices</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Woojin</au><au>Choi, Dongkil</au><au>Kim, Jung-Hyun</au><au>Koh, Won-Gun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays</atitle><jtitle>Biomedical microdevices</jtitle><stitle>Biomed Microdevices</stitle><addtitle>Biomed Microdevices</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>10</volume><issue>6</issue><spage>813</spage><epage>822</epage><pages>813-822</pages><issn>1387-2176</issn><eissn>1572-8781</eissn><coden>BMICFC</coden><abstract>Suspension arrays for protein-based assays have been developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles to overcome the problems with current systems which use color-coded rigid microparticles as protein supports. Various shapes of hydrogel microparticles were fabricated by a two-step process consisting of photopatterning and flushing using a poly(dimethylsiloxane) (PDMS) channel as a molding insert. Hydrogel microparticles with lateral dimensions ranging from 50 to 300 μm were fabricated using different molecular weights of PEG (700, 3,400, and 8,000 Da), by which the water content and swelling behavior of the hydrogel microparticles could be controlled. Protein-entrapped hydrogel microparticles were prepared in a suspension array format, and PEG hydrogel could encapsulate proteins without deactivation for a week due to its high water content and soft nature. The sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) was successfully investigated using fluorescence detection, demonstrating one possible application of suspension arrays. Furthermore, a mixture of two different shapes of hydrogel microparticles containing GOX/POD and alkaline phosphatase (AP), respectively, was prepared and the shape-coded suspension array was used for simultaneous characterization of two different enzyme-catalyzed reactions.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>18561028</pmid><doi>10.1007/s10544-008-9196-1</doi><tpages>10</tpages></addata></record> |
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| subjects | Alkaline Phosphatase - chemistry Bioassays Biological and Medical Physics Biological Assay - instrumentation Biological Assay - methods Biomedical Engineering and Bioengineering Biomedical research Biophysics Dimethylpolysiloxanes - chemistry Engineering Engineering Fluid Dynamics Enzymes Enzymes, Immobilized - chemistry Fluorescence Glucose Oxidase - chemistry Hydrogels - chemistry Nanoparticles Nanotechnology Particle Size Peroxidase - chemistry Polyethylene Glycols - chemistry Scientific method Suspensions |
| title | Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays |
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