Microfluidic handling of PCR solution and DNA amplification on a reaction chamber array biochip
A microfluidic biochip for conducting an array of polymerase chain reaction (PCR) simultaneously was fabricated to understand the microfluidic loading process of PCR solution into microfabricated glass reaction chambers. The geometrical factors of the microfluidic structure, including the shape and...
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| Veröffentlicht in: | Biomedical microdevices 2006-06, Vol.8 (2), p.167-176 |
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| creator | Gong, Haiqing Ramalingam, Naveen Chen, Longqing Che, Jing Wang, Qinghui Wang, Yuming Yang, Xinhao Yap, Peng Huat Eric Neo, Chiew Hoon |
| description | A microfluidic biochip for conducting an array of polymerase chain reaction (PCR) simultaneously was fabricated to understand the microfluidic loading process of PCR solution into microfabricated glass reaction chambers. The geometrical factors of the microfluidic structure, including the shape and depth of the microchamber, shape and size of the microchannels were investigated on the formation of air bubbles trapped within the microchamber during the PCR solution loading process. Furthermore, the effects of surface properties of the microfluidic structure, including hydrophilicity of the microchamber and inlet channel, and hydrophobicity of the outlet channel, on the loading of PCR solution, especially on the formation of air bubbles were studied. As a result, the surface wetting property of the microchamber was found to be the main reason for the formation of the air bubbles inside the microchamber during the loading of PCR solution in the biochips. A solution to avoid the air trapping has been proposed and investigated. |
| doi_str_mv | 10.1007/s10544-006-7712-8 |
| format | Article |
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The geometrical factors of the microfluidic structure, including the shape and depth of the microchamber, shape and size of the microchannels were investigated on the formation of air bubbles trapped within the microchamber during the PCR solution loading process. Furthermore, the effects of surface properties of the microfluidic structure, including hydrophilicity of the microchamber and inlet channel, and hydrophobicity of the outlet channel, on the loading of PCR solution, especially on the formation of air bubbles were studied. As a result, the surface wetting property of the microchamber was found to be the main reason for the formation of the air bubbles inside the microchamber during the loading of PCR solution in the biochips. A solution to avoid the air trapping has been proposed and investigated.</description><identifier>ISSN: 1387-2176</identifier><identifier>EISSN: 1572-8781</identifier><identifier>DOI: 10.1007/s10544-006-7712-8</identifier><identifier>PMID: 16688576</identifier><identifier>CODEN: BMICFC</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>DNA - genetics ; DNA - isolation & purification ; Equipment Design ; Equipment Failure Analysis ; Flow Injection Analysis - instrumentation ; Flow Injection Analysis - methods ; Microfluidic Analytical Techniques - instrumentation ; Microfluidic Analytical Techniques - methods ; Nucleic Acid Amplification Techniques - instrumentation ; Oligonucleotide Array Sequence Analysis - instrumentation ; Oligonucleotide Array Sequence Analysis - methods ; Polymerase Chain Reaction - instrumentation ; Polymerase Chain Reaction - methods ; Specimen Handling - instrumentation ; Specimen Handling - methods</subject><ispartof>Biomedical microdevices, 2006-06, Vol.8 (2), p.167-176</ispartof><rights>Springer Science + Business Media, LLC 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-d7e0f37d3b0b7809f427da18174f289f00ad9aa3a5976373ec64a4ca11dda7b3</citedby><cites>FETCH-LOGICAL-c455t-d7e0f37d3b0b7809f427da18174f289f00ad9aa3a5976373ec64a4ca11dda7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16688576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Haiqing</creatorcontrib><creatorcontrib>Ramalingam, Naveen</creatorcontrib><creatorcontrib>Chen, Longqing</creatorcontrib><creatorcontrib>Che, Jing</creatorcontrib><creatorcontrib>Wang, Qinghui</creatorcontrib><creatorcontrib>Wang, Yuming</creatorcontrib><creatorcontrib>Yang, Xinhao</creatorcontrib><creatorcontrib>Yap, Peng Huat Eric</creatorcontrib><creatorcontrib>Neo, Chiew Hoon</creatorcontrib><title>Microfluidic handling of PCR solution and DNA amplification on a reaction chamber array biochip</title><title>Biomedical microdevices</title><addtitle>Biomed Microdevices</addtitle><description>A microfluidic biochip for conducting an array of polymerase chain reaction (PCR) simultaneously was fabricated to understand the microfluidic loading process of PCR solution into microfabricated glass reaction chambers. 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Academic</collection><jtitle>Biomedical microdevices</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Haiqing</au><au>Ramalingam, Naveen</au><au>Chen, Longqing</au><au>Che, Jing</au><au>Wang, Qinghui</au><au>Wang, Yuming</au><au>Yang, Xinhao</au><au>Yap, Peng Huat Eric</au><au>Neo, Chiew Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic handling of PCR solution and DNA amplification on a reaction chamber array biochip</atitle><jtitle>Biomedical microdevices</jtitle><addtitle>Biomed Microdevices</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>8</volume><issue>2</issue><spage>167</spage><epage>176</epage><pages>167-176</pages><issn>1387-2176</issn><eissn>1572-8781</eissn><coden>BMICFC</coden><abstract>A microfluidic biochip for conducting an array of polymerase chain reaction (PCR) simultaneously was fabricated to understand the microfluidic loading process of PCR solution into microfabricated glass reaction chambers. The geometrical factors of the microfluidic structure, including the shape and depth of the microchamber, shape and size of the microchannels were investigated on the formation of air bubbles trapped within the microchamber during the PCR solution loading process. Furthermore, the effects of surface properties of the microfluidic structure, including hydrophilicity of the microchamber and inlet channel, and hydrophobicity of the outlet channel, on the loading of PCR solution, especially on the formation of air bubbles were studied. As a result, the surface wetting property of the microchamber was found to be the main reason for the formation of the air bubbles inside the microchamber during the loading of PCR solution in the biochips. A solution to avoid the air trapping has been proposed and investigated.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>16688576</pmid><doi>10.1007/s10544-006-7712-8</doi><tpages>10</tpages></addata></record> |
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| ispartof | Biomedical microdevices, 2006-06, Vol.8 (2), p.167-176 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | DNA - genetics DNA - isolation & purification Equipment Design Equipment Failure Analysis Flow Injection Analysis - instrumentation Flow Injection Analysis - methods Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Nucleic Acid Amplification Techniques - instrumentation Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Specimen Handling - instrumentation Specimen Handling - methods |
| title | Microfluidic handling of PCR solution and DNA amplification on a reaction chamber array biochip |
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