Laser ablation of micropores for formation of artificial planar lipid bilayers

Artificial lipid bilayers are a powerful tool for studying synthetic or reconstituted ion channels. Key to forming these lipid bilayers is having a small aperture in a septum separating two solution chambers. Traditional methods of aperture generation involve manually punching the aperture into the...

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Veröffentlicht in:Biomedical microdevices 2007-12, Vol.9 (6), p.863-868
Hauptverfasser: O'Shaughnessy, Thomas J, Hu, Jenny E, Kulp, 3rd, John L, Daly, Susan M, Ligler, Frances S
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container_end_page 868
container_issue 6
container_start_page 863
container_title Biomedical microdevices
container_volume 9
creator O'Shaughnessy, Thomas J
Hu, Jenny E
Kulp, 3rd, John L
Daly, Susan M
Ligler, Frances S
description Artificial lipid bilayers are a powerful tool for studying synthetic or reconstituted ion channels. Key to forming these lipid bilayers is having a small aperture in a septum separating two solution chambers. Traditional methods of aperture generation involve manually punching the aperture into the septum. While these techniques work, they are difficult to implement reliably and do not produce consistently sized apertures. Presented here is a method of using a UV excimer laser with a nanosecond scale pulse width to laser ablate apertures from 4 to 105 microm in 20 microm thick polycarbonate films for use in artificial lipid bilayer experiments. The data demonstrate that the apertures produced by laser ablation are highly reproducible and can support both the formation of stable, long-lasting lipid bilayers as well as the recording of ion channels incorporated into the bilayers.
doi_str_mv 10.1007/s10544-007-9099-6
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subjects Biomimetic Materials - chemistry
Biomimetic Materials - radiation effects
Biotechnology
Ions
Lasers
Lipid Bilayers - chemistry
Lipid Bilayers - radiation effects
Lipids
Materials Testing
Medical technology
Membranes, Artificial
Molecular Conformation
Polycarboxylate Cement - chemistry
Polycarboxylate Cement - radiation effects
Porosity
title Laser ablation of micropores for formation of artificial planar lipid bilayers
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