Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells
The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm 2 u...
Gespeichert in:
| Veröffentlicht in: | Lasers in medical science 2010-01, Vol.25 (1), p.33-39 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 39 |
|---|---|
| container_issue | 1 |
| container_start_page | 33 |
| container_title | Lasers in medical science |
| container_volume | 25 |
| creator | Mvula, B. Moore, T. J. Abrahamse, H. |
| description | The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm
2
using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm
2
on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro. |
| doi_str_mv | 10.1007/s10103-008-0636-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_743596490</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>743596490</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-f32e200b82cf8fe0f87bf4f922fa5beab22d02a380576138e34e56d410a3c70c3</originalsourceid><addsrcrecordid>eNqFkUFrFTEQx4Mo9rX6AbxI8KCn6EyS3c0epVQrFLwoeAvZ3Um7Nbt5Jrst_fZmeQ8UQT0lML_5ZyY_xl4gvEWA5l1GQFACwAioVS3wEduhVpWoQX97zHYgayNMK_GEneZ8C4BNjeopO8EWG6m03rHvF95Tv_DoeYj3ItAdBR5cpsTHlNwwumWMM3fzwGk_DpQmF_h1ivfLDfeuX2LiW3lYw8Jv1slt93EfM4nCjnc08LzQxHsKIT9jT7wLmZ4fzzP29cPFl_NLcfX546fz91ei1227CK8kSYDOyN4bT-BN03ntWym9qzpynZQDSKcMVNs6hpSmqh40glN9A706Y28OufsUf6yUFzuNeZvAzRTXbJvyRW2tW_g_qTRWVaU38vU_SYmygVapAr76A7yNa5rLvhZbI0FJ1RQID1CfYs6JvN2ncXLpwSLYTa09qLVFrd3UWiw9L4_BazfR8Kvj6LIA8gDkUpqvKf328l9TfwJQr64o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>198203237</pqid></control><display><type>article</type><title>Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Mvula, B. ; Moore, T. J. ; Abrahamse, H.</creator><creatorcontrib>Mvula, B. ; Moore, T. J. ; Abrahamse, H.</creatorcontrib><description>The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm
2
using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm
2
on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-008-0636-1</identifier><identifier>PMID: 19172344</identifier><identifier>CODEN: LMSCEZ</identifier><language>eng</language><publisher>London: Springer-Verlag</publisher><subject>Adenosine Triphosphate - metabolism ; Adipose Tissue - cytology ; Adult Stem Cells - cytology ; Adult Stem Cells - drug effects ; Adult Stem Cells - metabolism ; Adult Stem Cells - radiation effects ; Biomarkers - metabolism ; Cell growth ; Cell Proliferation - drug effects ; Cell Proliferation - radiation effects ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Cells, Cultured ; Clinical outcomes ; Dentistry ; Epidermal Growth Factor - pharmacology ; Humans ; Lasers ; Lasers, Semiconductor - therapeutic use ; Low-Level Light Therapy ; Luminescence ; Medicine ; Medicine & Public Health ; Optical Devices ; Optics ; Original Article ; Photonics ; Proteins ; Quantum Optics ; Stem cells ; Thy-1 Antigens - metabolism</subject><ispartof>Lasers in medical science, 2010-01, Vol.25 (1), p.33-39</ispartof><rights>Springer-Verlag London Ltd 2009</rights><rights>Springer-Verlag London Ltd 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-f32e200b82cf8fe0f87bf4f922fa5beab22d02a380576138e34e56d410a3c70c3</citedby><cites>FETCH-LOGICAL-c499t-f32e200b82cf8fe0f87bf4f922fa5beab22d02a380576138e34e56d410a3c70c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-008-0636-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-008-0636-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19172344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mvula, B.</creatorcontrib><creatorcontrib>Moore, T. J.</creatorcontrib><creatorcontrib>Abrahamse, H.</creatorcontrib><title>Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm
2
using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm
2
on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Adipose Tissue - cytology</subject><subject>Adult Stem Cells - cytology</subject><subject>Adult Stem Cells - drug effects</subject><subject>Adult Stem Cells - metabolism</subject><subject>Adult Stem Cells - radiation effects</subject><subject>Biomarkers - metabolism</subject><subject>Cell growth</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Proliferation - radiation effects</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Cells, Cultured</subject><subject>Clinical outcomes</subject><subject>Dentistry</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Humans</subject><subject>Lasers</subject><subject>Lasers, Semiconductor - therapeutic use</subject><subject>Low-Level Light Therapy</subject><subject>Luminescence</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Proteins</subject><subject>Quantum Optics</subject><subject>Stem cells</subject><subject>Thy-1 Antigens - metabolism</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUFrFTEQx4Mo9rX6AbxI8KCn6EyS3c0epVQrFLwoeAvZ3Um7Nbt5Jrst_fZmeQ8UQT0lML_5ZyY_xl4gvEWA5l1GQFACwAioVS3wEduhVpWoQX97zHYgayNMK_GEneZ8C4BNjeopO8EWG6m03rHvF95Tv_DoeYj3ItAdBR5cpsTHlNwwumWMM3fzwGk_DpQmF_h1ivfLDfeuX2LiW3lYw8Jv1slt93EfM4nCjnc08LzQxHsKIT9jT7wLmZ4fzzP29cPFl_NLcfX546fz91ei1227CK8kSYDOyN4bT-BN03ntWym9qzpynZQDSKcMVNs6hpSmqh40glN9A706Y28OufsUf6yUFzuNeZvAzRTXbJvyRW2tW_g_qTRWVaU38vU_SYmygVapAr76A7yNa5rLvhZbI0FJ1RQID1CfYs6JvN2ncXLpwSLYTa09qLVFrd3UWiw9L4_BazfR8Kvj6LIA8gDkUpqvKf328l9TfwJQr64o</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Mvula, B.</creator><creator>Moore, T. J.</creator><creator>Abrahamse, H.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells</title><author>Mvula, B. ; Moore, T. J. ; Abrahamse, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-f32e200b82cf8fe0f87bf4f922fa5beab22d02a380576138e34e56d410a3c70c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Adipose Tissue - cytology</topic><topic>Adult Stem Cells - cytology</topic><topic>Adult Stem Cells - drug effects</topic><topic>Adult Stem Cells - metabolism</topic><topic>Adult Stem Cells - radiation effects</topic><topic>Biomarkers - metabolism</topic><topic>Cell growth</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Proliferation - radiation effects</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Cells, Cultured</topic><topic>Clinical outcomes</topic><topic>Dentistry</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Humans</topic><topic>Lasers</topic><topic>Lasers, Semiconductor - therapeutic use</topic><topic>Low-Level Light Therapy</topic><topic>Luminescence</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Proteins</topic><topic>Quantum Optics</topic><topic>Stem cells</topic><topic>Thy-1 Antigens - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mvula, B.</creatorcontrib><creatorcontrib>Moore, T. J.</creatorcontrib><creatorcontrib>Abrahamse, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mvula, B.</au><au>Moore, T. J.</au><au>Abrahamse, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>25</volume><issue>1</issue><spage>33</spage><epage>39</epage><pages>33-39</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><coden>LMSCEZ</coden><abstract>The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm
2
using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm
2
on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.</abstract><cop>London</cop><pub>Springer-Verlag</pub><pmid>19172344</pmid><doi>10.1007/s10103-008-0636-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-8921 |
| ispartof | Lasers in medical science, 2010-01, Vol.25 (1), p.33-39 |
| issn | 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743596490 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adenosine Triphosphate - metabolism Adipose Tissue - cytology Adult Stem Cells - cytology Adult Stem Cells - drug effects Adult Stem Cells - metabolism Adult Stem Cells - radiation effects Biomarkers - metabolism Cell growth Cell Proliferation - drug effects Cell Proliferation - radiation effects Cell Survival - drug effects Cell Survival - radiation effects Cells, Cultured Clinical outcomes Dentistry Epidermal Growth Factor - pharmacology Humans Lasers Lasers, Semiconductor - therapeutic use Low-Level Light Therapy Luminescence Medicine Medicine & Public Health Optical Devices Optics Original Article Photonics Proteins Quantum Optics Stem cells Thy-1 Antigens - metabolism |
| title | Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T11%3A41%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20low-level%20laser%20irradiation%20and%20epidermal%20growth%20factor%20on%20adult%20human%20adipose-derived%20stem%20cells&rft.jtitle=Lasers%20in%20medical%20science&rft.au=Mvula,%20B.&rft.date=2010-01-01&rft.volume=25&rft.issue=1&rft.spage=33&rft.epage=39&rft.pages=33-39&rft.issn=0268-8921&rft.eissn=1435-604X&rft.coden=LMSCEZ&rft_id=info:doi/10.1007/s10103-008-0636-1&rft_dat=%3Cproquest_cross%3E743596490%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=198203237&rft_id=info:pmid/19172344&rfr_iscdi=true |