Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea
The soil nitrifying bacterium Nitrosomonas europaea has shown the ability to transform cometabolically naphthalene as well as other 2- and 3-ringed polycyclic aromatic hydrocarbons (PAHs) to more oxidized products. All of the observed enzymatic reactions were inhibited by acetylene, a selective inhi...
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| Veröffentlicht in: | Biodegradation (Dordrecht) 2002, Vol.13 (6), p.373-381 |
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| creator | CHANG, Soon W HYMAN, Michael R WILLIAMSON, Kenneth J |
| description | The soil nitrifying bacterium Nitrosomonas europaea has shown the ability to transform cometabolically naphthalene as well as other 2- and 3-ringed polycyclic aromatic hydrocarbons (PAHs) to more oxidized products. All of the observed enzymatic reactions were inhibited by acetylene, a selective inhibitor of ammonia monooxygenase (AMO). A strong inhibitory effect of naphthalene on ammonia oxidation by N. europaea was observed. Naphthalene was readily oxidized by N. europaea and 2-naphthol was detected as a major product (85%) of naphthalene oxidation. The maximum naphthol production rate was 1.65 nmole/mg protein-min in the presence of 240 microM naphthalene and 10 mM NH4+. Our results demonstrate that the oxidation between ammonia and naphthalene showed a partial competitive inhibition. The relative ratio of naphthalene and ammonia oxidation, depending on naphthalene concentrations, demonstrated that the naphthalene was oxidized 2200-fold slower than ammonia at lower concentration of naphthalene (15 microM) whereas naphthalene was oxidized only 100-fold slower than ammonia oxidation. NH4(+)- and N2H4-dependent O2 uptake measurement demonstrated irreversible inhibitory effects of the naphthalene and subsequent oxidation products on AMO and HAO activity. |
| doi_str_mv | 10.1023/A:1022811430030 |
| format | Article |
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All of the observed enzymatic reactions were inhibited by acetylene, a selective inhibitor of ammonia monooxygenase (AMO). A strong inhibitory effect of naphthalene on ammonia oxidation by N. europaea was observed. Naphthalene was readily oxidized by N. europaea and 2-naphthol was detected as a major product (85%) of naphthalene oxidation. The maximum naphthol production rate was 1.65 nmole/mg protein-min in the presence of 240 microM naphthalene and 10 mM NH4+. Our results demonstrate that the oxidation between ammonia and naphthalene showed a partial competitive inhibition. The relative ratio of naphthalene and ammonia oxidation, depending on naphthalene concentrations, demonstrated that the naphthalene was oxidized 2200-fold slower than ammonia at lower concentration of naphthalene (15 microM) whereas naphthalene was oxidized only 100-fold slower than ammonia oxidation. NH4(+)- and N2H4-dependent O2 uptake measurement demonstrated irreversible inhibitory effects of the naphthalene and subsequent oxidation products on AMO and HAO activity.</description><identifier>ISSN: 0923-9820</identifier><identifier>EISSN: 1572-9729</identifier><identifier>DOI: 10.1023/A:1022811430030</identifier><identifier>PMID: 12713129</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Acetylene - pharmacology ; Ammonia ; Ammonia - metabolism ; ammonia monooxygenase ; Bacteria ; Binding, Competitive ; Biodegradation, Environmental ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Kinetics ; Microbiology ; Naphthalene ; Naphthalenes - metabolism ; Naphthalenes - pharmacology ; Nitrosomonas europaea ; Nitrosomonas europaea - metabolism ; Oxidation ; Oxidoreductases - antagonists & inhibitors ; Oxidoreductases - metabolism ; Oxygen - metabolism ; Polycyclic aromatic hydrocarbons ; Polycyclic Aromatic Hydrocarbons - metabolism ; Soil Microbiology</subject><ispartof>Biodegradation (Dordrecht), 2002, Vol.13 (6), p.373-381</ispartof><rights>2003 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-7a0bc31a86a3b5be18e51943fd5319e760e68e42e3a3a2f129fddc4cc24cc56c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14705394$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12713129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHANG, Soon W</creatorcontrib><creatorcontrib>HYMAN, Michael R</creatorcontrib><creatorcontrib>WILLIAMSON, Kenneth J</creatorcontrib><title>Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea</title><title>Biodegradation (Dordrecht)</title><addtitle>Biodegradation</addtitle><description>The soil nitrifying bacterium Nitrosomonas europaea has shown the ability to transform cometabolically naphthalene as well as other 2- and 3-ringed polycyclic aromatic hydrocarbons (PAHs) to more oxidized products. All of the observed enzymatic reactions were inhibited by acetylene, a selective inhibitor of ammonia monooxygenase (AMO). A strong inhibitory effect of naphthalene on ammonia oxidation by N. europaea was observed. Naphthalene was readily oxidized by N. europaea and 2-naphthol was detected as a major product (85%) of naphthalene oxidation. The maximum naphthol production rate was 1.65 nmole/mg protein-min in the presence of 240 microM naphthalene and 10 mM NH4+. Our results demonstrate that the oxidation between ammonia and naphthalene showed a partial competitive inhibition. The relative ratio of naphthalene and ammonia oxidation, depending on naphthalene concentrations, demonstrated that the naphthalene was oxidized 2200-fold slower than ammonia at lower concentration of naphthalene (15 microM) whereas naphthalene was oxidized only 100-fold slower than ammonia oxidation. NH4(+)- and N2H4-dependent O2 uptake measurement demonstrated irreversible inhibitory effects of the naphthalene and subsequent oxidation products on AMO and HAO activity.</description><subject>Acetylene - pharmacology</subject><subject>Ammonia</subject><subject>Ammonia - metabolism</subject><subject>ammonia monooxygenase</subject><subject>Bacteria</subject><subject>Binding, Competitive</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>Microbiology</subject><subject>Naphthalene</subject><subject>Naphthalenes - metabolism</subject><subject>Naphthalenes - pharmacology</subject><subject>Nitrosomonas europaea</subject><subject>Nitrosomonas europaea - metabolism</subject><subject>Oxidation</subject><subject>Oxidoreductases - antagonists & inhibitors</subject><subject>Oxidoreductases - metabolism</subject><subject>Oxygen - metabolism</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Polycyclic Aromatic Hydrocarbons - metabolism</subject><subject>Soil Microbiology</subject><issn>0923-9820</issn><issn>1572-9729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0c1rFDEUAPAgit1Wz71JENpeHM3nZNJbWdoqFL3oeXmTeeOmzCRjMgOd_74pbhE86CE8CD_eJyGnnH3kTMhPV5cliIZzJRmT7AXZcG1EZY2wL8mGWSEr2wh2RI5zvmeMWcPEa3LEheGSC7shD9sYH3wHs4-Bxp4GmPbzHgYMSCF0NM57THSKw-pWN3hHIcWxaEf3a5eig9TGkGm70gJp8HPy_erDT9qCmzH5ZfxAv5bfmOMYA2SKS4oTILwhr3oYMr49xBPy4-b6-_Zzdfft9sv26q5yiqm5MsBaJzk0NchWt8gb1Nwq2XdacoumZlg3qARKkCD6MlPfdU45J8rTtZMn5OJ33inFXwvmeTf67HAYIGBc8s4oqa3Wqi7y_J-SWyuZafT_oapLj_wJvv8L3sclhTLuU9lGCM2bgt4d0NKO2O2m5EdI6-75RgWcHQBkB0OfIDif_zhlmJZlJY81IqHH</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>CHANG, Soon W</creator><creator>HYMAN, Michael R</creator><creator>WILLIAMSON, Kenneth J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>SOI</scope><scope>7QO</scope><scope>7TV</scope><scope>KR7</scope></search><sort><creationdate>2002</creationdate><title>Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea</title><author>CHANG, Soon W ; HYMAN, Michael R ; WILLIAMSON, Kenneth J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-7a0bc31a86a3b5be18e51943fd5319e760e68e42e3a3a2f129fddc4cc24cc56c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acetylene - pharmacology</topic><topic>Ammonia</topic><topic>Ammonia - metabolism</topic><topic>ammonia monooxygenase</topic><topic>Bacteria</topic><topic>Binding, Competitive</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kinetics</topic><topic>Microbiology</topic><topic>Naphthalene</topic><topic>Naphthalenes - metabolism</topic><topic>Naphthalenes - pharmacology</topic><topic>Nitrosomonas europaea</topic><topic>Nitrosomonas europaea - metabolism</topic><topic>Oxidation</topic><topic>Oxidoreductases - antagonists & inhibitors</topic><topic>Oxidoreductases - metabolism</topic><topic>Oxygen - metabolism</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Polycyclic Aromatic Hydrocarbons - metabolism</topic><topic>Soil Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHANG, Soon W</creatorcontrib><creatorcontrib>HYMAN, Michael R</creatorcontrib><creatorcontrib>WILLIAMSON, Kenneth J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE 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(Dordrecht)</jtitle><addtitle>Biodegradation</addtitle><date>2002</date><risdate>2002</risdate><volume>13</volume><issue>6</issue><spage>373</spage><epage>381</epage><pages>373-381</pages><issn>0923-9820</issn><eissn>1572-9729</eissn><abstract>The soil nitrifying bacterium Nitrosomonas europaea has shown the ability to transform cometabolically naphthalene as well as other 2- and 3-ringed polycyclic aromatic hydrocarbons (PAHs) to more oxidized products. All of the observed enzymatic reactions were inhibited by acetylene, a selective inhibitor of ammonia monooxygenase (AMO). A strong inhibitory effect of naphthalene on ammonia oxidation by N. europaea was observed. Naphthalene was readily oxidized by N. europaea and 2-naphthol was detected as a major product (85%) of naphthalene oxidation. The maximum naphthol production rate was 1.65 nmole/mg protein-min in the presence of 240 microM naphthalene and 10 mM NH4+. Our results demonstrate that the oxidation between ammonia and naphthalene showed a partial competitive inhibition. The relative ratio of naphthalene and ammonia oxidation, depending on naphthalene concentrations, demonstrated that the naphthalene was oxidized 2200-fold slower than ammonia at lower concentration of naphthalene (15 microM) whereas naphthalene was oxidized only 100-fold slower than ammonia oxidation. NH4(+)- and N2H4-dependent O2 uptake measurement demonstrated irreversible inhibitory effects of the naphthalene and subsequent oxidation products on AMO and HAO activity.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>12713129</pmid><doi>10.1023/A:1022811430030</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biodegradation (Dordrecht), 2002, Vol.13 (6), p.373-381 |
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| subjects | Acetylene - pharmacology Ammonia Ammonia - metabolism ammonia monooxygenase Bacteria Binding, Competitive Biodegradation, Environmental Biological and medical sciences Fundamental and applied biological sciences. Psychology Kinetics Microbiology Naphthalene Naphthalenes - metabolism Naphthalenes - pharmacology Nitrosomonas europaea Nitrosomonas europaea - metabolism Oxidation Oxidoreductases - antagonists & inhibitors Oxidoreductases - metabolism Oxygen - metabolism Polycyclic aromatic hydrocarbons Polycyclic Aromatic Hydrocarbons - metabolism Soil Microbiology |
| title | Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea |
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