Auxin transport inhibitors block PIN1 cycling and vesicle trafficking
Polar transport of the phytohormone auxin mediates various processes in plant growth and development, such as apical dominance, tropisms, vascular patterning and axis formation 1 , 2 . This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux...
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| Veröffentlicht in: | Nature (London) 2001-09, Vol.413 (6854), p.425-428 |
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| creator | Geldner, Niko Friml, Jiří Stierhof, York-Dieter Jürgens, Gerd Palme, Klaus |
| description | Polar transport of the phytohormone auxin mediates various processes in plant growth and development, such as apical dominance, tropisms, vascular patterning and axis formation
1
,
2
. This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux from the cell but their mode of action is unknown
3
. It is thought that polar auxin flux is caused by the asymmetric distribution of efflux carriers acting at the plasma membrane
4
. The polar localization of efflux carrier candidate PIN1 supports this model
4
. Here we show that the seemingly static localization of PIN1 results from rapid actin-dependent cycling between the plasma membrane and endosomal compartments. Auxin transport inhibitors block PIN1 cycling and inhibit trafficking of membrane proteins that are unrelated to auxin transport. Our data suggest that PIN1 cycling is of central importance for auxin transport and that auxin transport inhibitors affect efflux by generally interfering with membrane-trafficking processes. In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors. |
| doi_str_mv | 10.1038/35096571 |
| format | Article |
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1
,
2
. This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux from the cell but their mode of action is unknown
3
. It is thought that polar auxin flux is caused by the asymmetric distribution of efflux carriers acting at the plasma membrane
4
. The polar localization of efflux carrier candidate PIN1 supports this model
4
. Here we show that the seemingly static localization of PIN1 results from rapid actin-dependent cycling between the plasma membrane and endosomal compartments. Auxin transport inhibitors block PIN1 cycling and inhibit trafficking of membrane proteins that are unrelated to auxin transport. Our data suggest that PIN1 cycling is of central importance for auxin transport and that auxin transport inhibitors affect efflux by generally interfering with membrane-trafficking processes. In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/35096571</identifier><identifier>PMID: 11574889</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Arabidopsis ; Arabidopsis Proteins ; Biological and medical sciences ; Biological Transport ; Brefeldin A - pharmacology ; Cell Membrane - metabolism ; Cells ; Cytoskeleton - metabolism ; Endosomes - metabolism ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Hormones ; Humanities and Social Sciences ; Indoleacetic Acids - antagonists & inhibitors ; Indoleacetic Acids - metabolism ; Inhibitors ; letter ; Membrane Proteins - metabolism ; Membrane Transport Proteins ; Membranes ; Mode of action ; multidisciplinary ; Plant growth ; Plant growth. Development of the storage organs ; Plant physiology and development ; Proton-Translocating ATPases - metabolism ; Science ; Science (multidisciplinary) ; Triiodobenzoic Acids - pharmacology ; Vegetative apparatus, growth and morphogenesis. Senescence</subject><ispartof>Nature (London), 2001-09, Vol.413 (6854), p.425-428</ispartof><rights>Macmillan Magazines Ltd. 2001</rights><rights>2001 INIST-CNRS</rights><rights>COPYRIGHT 2001 Nature Publishing Group</rights><rights>Copyright Macmillan Journals Ltd. Sep 27, 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5561-92d10b2d05e690c430c4aa3502d7a8c7bb126ef2f396e14450c3df69be7c357a3</citedby><cites>FETCH-LOGICAL-c5561-92d10b2d05e690c430c4aa3502d7a8c7bb126ef2f396e14450c3df69be7c357a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/35096571$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/35096571$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1098493$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11574889$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geldner, Niko</creatorcontrib><creatorcontrib>Friml, Jiří</creatorcontrib><creatorcontrib>Stierhof, York-Dieter</creatorcontrib><creatorcontrib>Jürgens, Gerd</creatorcontrib><creatorcontrib>Palme, Klaus</creatorcontrib><title>Auxin transport inhibitors block PIN1 cycling and vesicle trafficking</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Polar transport of the phytohormone auxin mediates various processes in plant growth and development, such as apical dominance, tropisms, vascular patterning and axis formation
1
,
2
. This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux from the cell but their mode of action is unknown
3
. It is thought that polar auxin flux is caused by the asymmetric distribution of efflux carriers acting at the plasma membrane
4
. The polar localization of efflux carrier candidate PIN1 supports this model
4
. Here we show that the seemingly static localization of PIN1 results from rapid actin-dependent cycling between the plasma membrane and endosomal compartments. Auxin transport inhibitors block PIN1 cycling and inhibit trafficking of membrane proteins that are unrelated to auxin transport. Our data suggest that PIN1 cycling is of central importance for auxin transport and that auxin transport inhibitors affect efflux by generally interfering with membrane-trafficking processes. In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.</description><subject>Arabidopsis</subject><subject>Arabidopsis Proteins</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Brefeldin A - pharmacology</subject><subject>Cell Membrane - metabolism</subject><subject>Cells</subject><subject>Cytoskeleton - metabolism</subject><subject>Endosomes - metabolism</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones</subject><subject>Humanities and Social Sciences</subject><subject>Indoleacetic Acids - antagonists & inhibitors</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Inhibitors</subject><subject>letter</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Transport Proteins</subject><subject>Membranes</subject><subject>Mode of action</subject><subject>multidisciplinary</subject><subject>Plant growth</subject><subject>Plant growth. Development of the storage organs</subject><subject>Plant physiology and development</subject><subject>Proton-Translocating ATPases - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Triiodobenzoic Acids - pharmacology</subject><subject>Vegetative apparatus, growth and morphogenesis. 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1
,
2
. This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux from the cell but their mode of action is unknown
3
. It is thought that polar auxin flux is caused by the asymmetric distribution of efflux carriers acting at the plasma membrane
4
. The polar localization of efflux carrier candidate PIN1 supports this model
4
. Here we show that the seemingly static localization of PIN1 results from rapid actin-dependent cycling between the plasma membrane and endosomal compartments. Auxin transport inhibitors block PIN1 cycling and inhibit trafficking of membrane proteins that are unrelated to auxin transport. Our data suggest that PIN1 cycling is of central importance for auxin transport and that auxin transport inhibitors affect efflux by generally interfering with membrane-trafficking processes. In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>11574889</pmid><doi>10.1038/35096571</doi><tpages>4</tpages></addata></record> |
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| ispartof | Nature (London), 2001-09, Vol.413 (6854), p.425-428 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | MEDLINE; Nature; SpringerLink Journals - AutoHoldings |
| subjects | Arabidopsis Arabidopsis Proteins Biological and medical sciences Biological Transport Brefeldin A - pharmacology Cell Membrane - metabolism Cells Cytoskeleton - metabolism Endosomes - metabolism Flowers & plants Fundamental and applied biological sciences. Psychology Hormones Humanities and Social Sciences Indoleacetic Acids - antagonists & inhibitors Indoleacetic Acids - metabolism Inhibitors letter Membrane Proteins - metabolism Membrane Transport Proteins Membranes Mode of action multidisciplinary Plant growth Plant growth. Development of the storage organs Plant physiology and development Proton-Translocating ATPases - metabolism Science Science (multidisciplinary) Triiodobenzoic Acids - pharmacology Vegetative apparatus, growth and morphogenesis. Senescence |
| title | Auxin transport inhibitors block PIN1 cycling and vesicle trafficking |
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