Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells

A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using N...

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Veröffentlicht in:	Biotechnology letters 2007-10, Vol.29 (10), p.1459-1464
Hauptverfasser:	Li, Xiaoxiang, Wang, Xiaoli, Xiong, Shaoling, Zhang, Jing, Cai, Litao, Yang, Yanyan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bac-to-Bac Baculovirus Biological and medical sciences Biotechnology Cell Line Electrophoresis, Polyacrylamide Gel Expression Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic Genetic Vectors - genetics Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Nattokinase Proteins purification Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Resins Spodoptera Spodoptera frugiperda Subtilisins - genetics Subtilisins - isolation & purification Subtilisins - metabolism
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