Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements
Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3′,5′-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at tw...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1991-08, Vol.253 (5021), p.786-789 |
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| creator | Kapiloff, Michael S. Farkash, Yigal Wegner, Michael Rosenfeld, Michael G. |
| description | Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3′,5′-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr$^{220}$), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses. |
| doi_str_mv | 10.1126/science.1652153 |
| format | Article |
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Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr$^{220}$), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1652153</identifier><identifier>PMID: 1652153</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Amino Acid Sequence ; Base Sequence ; Binding Sites ; Biological and medical sciences ; Cell Line ; Cell lines ; Cellular biology ; Cyclic AMP - pharmacology ; Deoxyribonucleic acid ; DNA ; DNA - metabolism ; DNA binding proteins ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - physiology ; Fundamental and applied biological sciences. Psychology ; Gels ; Genes ; Genetic transcription ; Growth hormones ; Hormones ; In Vitro Techniques ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Peptide Mapping ; Phorbol esters ; Phosphorylation ; Phosphothreonine - metabolism ; Pituitary Gland - physiology ; Protein Kinases - metabolism ; Radioactive decay ; Regulatory Sequences, Nucleic Acid ; Sequencing ; Structure-Activity Relationship ; Tetradecanoylphorbol Acetate - pharmacology ; Transcription (Genetics) ; Transcription Factor Pit-1 ; Transcription Factors - chemistry ; Transcription Factors - physiology ; Transcription. Transcription factor. Splicing. Rna processing ; Transcriptional regulatory elements ; Trypsin</subject><ispartof>Science (American Association for the Advancement of Science), 1991-08, Vol.253 (5021), p.786-789</ispartof><rights>Copyright 1991 American Association for the Advancement of Science</rights><rights>1992 INIST-CNRS</rights><rights>COPYRIGHT 1991 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1991 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Aug 16, 1991</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c706t-f36e2a036dfa1fa0fdb28915de27bac5eb915a00e0849b9ba3bee07610bb3d093</citedby><cites>FETCH-LOGICAL-c706t-f36e2a036dfa1fa0fdb28915de27bac5eb915a00e0849b9ba3bee07610bb3d093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2879126$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2879126$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5149716$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1652153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kapiloff, Michael S.</creatorcontrib><creatorcontrib>Farkash, Yigal</creatorcontrib><creatorcontrib>Wegner, Michael</creatorcontrib><creatorcontrib>Rosenfeld, Michael G.</creatorcontrib><title>Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3′,5′-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr$^{220}$), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cellular biology</subject><subject>Cyclic AMP - pharmacology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>DNA binding proteins</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genes</subject><subject>Genetic transcription</subject><subject>Growth hormones</subject><subject>Hormones</subject><subject>In Vitro Techniques</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Peptide Mapping</subject><subject>Phorbol esters</subject><subject>Phosphorylation</subject><subject>Phosphothreonine - metabolism</subject><subject>Pituitary Gland - physiology</subject><subject>Protein Kinases - metabolism</subject><subject>Radioactive decay</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Sequencing</subject><subject>Structure-Activity Relationship</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Transcription (Genetics)</subject><subject>Transcription Factor Pit-1</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - physiology</subject><subject>Transcription. 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kapiloff, Michael S.</au><au>Farkash, Yigal</au><au>Wegner, Michael</au><au>Rosenfeld, Michael G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1991-08-16</date><risdate>1991</risdate><volume>253</volume><issue>5021</issue><spage>786</spage><epage>789</epage><pages>786-789</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3′,5′-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr$^{220}$), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>1652153</pmid><doi>10.1126/science.1652153</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1991-08, Vol.253 (5021), p.786-789 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743551943 |
| source | MEDLINE; Science Magazine; JSTOR Archive Collection A-Z Listing |
| subjects | Amino Acid Sequence Base Sequence Binding Sites Biological and medical sciences Cell Line Cell lines Cellular biology Cyclic AMP - pharmacology Deoxyribonucleic acid DNA DNA - metabolism DNA binding proteins DNA-Binding Proteins - chemistry DNA-Binding Proteins - physiology Fundamental and applied biological sciences. Psychology Gels Genes Genetic transcription Growth hormones Hormones In Vitro Techniques Molecular and cellular biology Molecular genetics Molecular Sequence Data Peptide Mapping Phorbol esters Phosphorylation Phosphothreonine - metabolism Pituitary Gland - physiology Protein Kinases - metabolism Radioactive decay Regulatory Sequences, Nucleic Acid Sequencing Structure-Activity Relationship Tetradecanoylphorbol Acetate - pharmacology Transcription (Genetics) Transcription Factor Pit-1 Transcription Factors - chemistry Transcription Factors - physiology Transcription. Transcription factor. Splicing. Rna processing Transcriptional regulatory elements Trypsin |
| title | Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements |
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