Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris
Recombinant hG-CSF was expressed in Pichia pastoris under the control of the AOX1 promoter. In this study, the glycerol feeding rate was adjusted to achieve the maximum attainable specific growth rate before induction. Using a two-stage glycerol feeding method, the specific growth rate was changed f...
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| Veröffentlicht in: | Biotechnology letters 2008-06, Vol.30 (6), p.1081-1085 |
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| container_title | Biotechnology letters |
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| creator | Bahrami, A Shojaosadati, S. A Khalilzadeh, R Farahani, E. Vasheghani |
| description | Recombinant hG-CSF was expressed in Pichia pastoris under the control of the AOX1 promoter. In this study, the glycerol feeding rate was adjusted to achieve the maximum attainable specific growth rate before induction. Using a two-stage glycerol feeding method, the specific growth rate was changed from a maximum value of 0.21 h⁻¹ (at the beginning of feeding) to 0.15 h⁻¹ prior to induction. With this approach, the final dry cell wt and rhG-CSF yield achieved was close to 120 g l⁻¹ and 320 mg l⁻¹, respectively. Our study found that the two-stage feeding method allowed the overall productivity of rhG-CSF to increase 2.9 times that of the conventional fed-batch method. |
| doi_str_mv | 10.1007/s10529-008-9650-4 |
| format | Article |
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A ; Khalilzadeh, R ; Farahani, E. Vasheghani</creator><creatorcontrib>Bahrami, A ; Shojaosadati, S. A ; Khalilzadeh, R ; Farahani, E. Vasheghani</creatorcontrib><description>Recombinant hG-CSF was expressed in Pichia pastoris under the control of the AOX1 promoter. In this study, the glycerol feeding rate was adjusted to achieve the maximum attainable specific growth rate before induction. Using a two-stage glycerol feeding method, the specific growth rate was changed from a maximum value of 0.21 h⁻¹ (at the beginning of feeding) to 0.15 h⁻¹ prior to induction. With this approach, the final dry cell wt and rhG-CSF yield achieved was close to 120 g l⁻¹ and 320 mg l⁻¹, respectively. Our study found that the two-stage feeding method allowed the overall productivity of rhG-CSF to increase 2.9 times that of the conventional fed-batch method.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-008-9650-4</identifier><identifier>PMID: 18246301</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Applied Microbiology ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Culture Media ; Cytokines ; Feeding rates ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Glycerol - metabolism ; Granulocyte Colony-Stimulating Factor - biosynthesis ; Humans ; Life Sciences ; Methanol ; Microbiology ; Original Research Paper ; Pichia - metabolism ; Pichia pastoris ; Proteins ; Recombinant Proteins</subject><ispartof>Biotechnology letters, 2008-06, Vol.30 (6), p.1081-1085</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-4893b60ce06bf04b532142ff27579b336c286f9059850bab7f4ec36ef6dd86c53</citedby><cites>FETCH-LOGICAL-c486t-4893b60ce06bf04b532142ff27579b336c286f9059850bab7f4ec36ef6dd86c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-008-9650-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-008-9650-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20306190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18246301$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bahrami, A</creatorcontrib><creatorcontrib>Shojaosadati, S. A</creatorcontrib><creatorcontrib>Khalilzadeh, R</creatorcontrib><creatorcontrib>Farahani, E. Vasheghani</creatorcontrib><title>Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Recombinant hG-CSF was expressed in Pichia pastoris under the control of the AOX1 promoter. In this study, the glycerol feeding rate was adjusted to achieve the maximum attainable specific growth rate before induction. Using a two-stage glycerol feeding method, the specific growth rate was changed from a maximum value of 0.21 h⁻¹ (at the beginning of feeding) to 0.15 h⁻¹ prior to induction. With this approach, the final dry cell wt and rhG-CSF yield achieved was close to 120 g l⁻¹ and 320 mg l⁻¹, respectively. Our study found that the two-stage feeding method allowed the overall productivity of rhG-CSF to increase 2.9 times that of the conventional fed-batch method.</description><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Culture Media</subject><subject>Cytokines</subject><subject>Feeding rates</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Glycerol - metabolism</subject><subject>Granulocyte Colony-Stimulating Factor - biosynthesis</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Methanol</subject><subject>Microbiology</subject><subject>Original Research Paper</subject><subject>Pichia - metabolism</subject><subject>Pichia pastoris</subject><subject>Proteins</subject><subject>Recombinant Proteins</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkV1rFDEUhoModq3-AG80COpV9OR7cimLrUJBoe11yGSS2SmzkzWZQfrvzTKLBS_qVQh53nPe8CD0msInCqA_FwqSGQLQEKMkEPEEbajUnCit1VO0ASookcKwM_SilDsAMBr0c3RGGyYUB7pBy83vRMrs-oD78d6HnEYcQ-iGqccxZRymnZt82IdpxiniHHzat8Pk6nV3SbbXF_iQU7f4eUgTHibsarojrZv9DvtlnJccjrmfg98NDh9cmVMeykv0LLqxhFen8xzdXny92X4jVz8uv2-_XBEvGjUT0RjeKvABVBtBtJIzKliMTEttWs6VZ42KBqRpJLSu1VEEz1WIqusa5SU_Rx_XubXkryWU2e6H4sM4uimkpVgtuBTcGFXJD4-SylCmRMP_CzJQFCiICr77B7xLS57qdy2jVBitKasQXSGfUyk5RHvIw97le0vBHh3b1bGtju3RsT0OfnMavLT70D0kTlIr8P4EuOLdGHM1OJS_HANeOxqoHFu5Up-mPuSHho9tf7uGokvW9VWmvb1mdWtlGsMk538Aa-jGXQ</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Bahrami, A</creator><creator>Shojaosadati, S. 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A ; Khalilzadeh, R ; Farahani, E. Vasheghani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-4893b60ce06bf04b532142ff27579b336c286f9059850bab7f4ec36ef6dd86c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Culture Media</topic><topic>Cytokines</topic><topic>Feeding rates</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. 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A</au><au>Khalilzadeh, R</au><au>Farahani, E. Vasheghani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>30</volume><issue>6</issue><spage>1081</spage><epage>1085</epage><pages>1081-1085</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Recombinant hG-CSF was expressed in Pichia pastoris under the control of the AOX1 promoter. In this study, the glycerol feeding rate was adjusted to achieve the maximum attainable specific growth rate before induction. Using a two-stage glycerol feeding method, the specific growth rate was changed from a maximum value of 0.21 h⁻¹ (at the beginning of feeding) to 0.15 h⁻¹ prior to induction. With this approach, the final dry cell wt and rhG-CSF yield achieved was close to 120 g l⁻¹ and 320 mg l⁻¹, respectively. Our study found that the two-stage feeding method allowed the overall productivity of rhG-CSF to increase 2.9 times that of the conventional fed-batch method.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>18246301</pmid><doi>10.1007/s10529-008-9650-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2008-06, Vol.30 (6), p.1081-1085 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Applied Microbiology Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Culture Media Cytokines Feeding rates Fermentation Fundamental and applied biological sciences. Psychology Gene expression Glycerol - metabolism Granulocyte Colony-Stimulating Factor - biosynthesis Humans Life Sciences Methanol Microbiology Original Research Paper Pichia - metabolism Pichia pastoris Proteins Recombinant Proteins |
| title | Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris |
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