Site-directed mutagenesis of the calcium-binding site of [alpha]-amylase of Bacillus licheniformis

Amylases that are active under acidic conditions (pH 70°C) and have less reliance on Ca^sup 2+^ are required for starch hydrolysis. The α-amylase gene of Bacillus licheniformis MTCC 6598 was cloned and expressed in Escherichia coli BL21. The calcium-binding site spanning amino acid residues from 104...

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Veröffentlicht in:	Biotechnology letters 2007-10, Vol.29 (10), p.1493-1499
Hauptverfasser:	Priyadharshini, Ramachandran, Gunasekaran, Paramasamy
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Bacillus licheniformis Binding sites Calcium E coli Escherichia coli High temperature Mutagenesis Mutants Mutation
Online-Zugang:	Volltext
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