Simultaneous Identification of Bacterial Virulence Genes by Negative Selection
An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplific...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1995-07, Vol.269 (5222), p.400-403 |
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| creator | Hensel, Michael Shea, Jacqueline E. Gleeson, Colin Jones, Michael D. Dalton, Emma Holden, David W. |
| description | An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium. |
| doi_str_mv | 10.1126/science.7618105 |
| format | Article |
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The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. 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Psychology ; Genes ; Genes, Bacterial ; Genetic aspects ; Genetic screening ; Genetics ; Identification and classification ; Inoculum ; Mice ; Mice, Inbred BALB C ; Microbiology ; Molecular Sequence Data ; Mutagenesis, Insertional ; Mutation ; Nucleic Acid Hybridization ; Pathogenic bacteria ; Pathogenic microorganisms ; Polymerase Chain Reaction ; Salmonella Infections, Animal - microbiology ; Salmonella typhimurium - genetics ; Salmonella typhimurium - pathogenicity ; Screening Tests ; Sequence Tagged Sites ; Spleen ; Transcriptional regulatory elements ; Transposons ; Virulence ; Virulence (Microbiology) ; Virulence - genetics ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1995-07, Vol.269 (5222), p.400-403</ispartof><rights>Copyright 1995 American Association for the Advancement of Science</rights><rights>1995 INIST-CNRS</rights><rights>COPYRIGHT 1995 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1995 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Jul 21, 1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c741t-72fbdda20a7b59d79f3b8492c9ff7c14fcbcf46d41db27512f9f18d7f69db04b3</citedby><cites>FETCH-LOGICAL-c741t-72fbdda20a7b59d79f3b8492c9ff7c14fcbcf46d41db27512f9f18d7f69db04b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2888285$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2888285$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3600660$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7618105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hensel, Michael</creatorcontrib><creatorcontrib>Shea, Jacqueline E.</creatorcontrib><creatorcontrib>Gleeson, Colin</creatorcontrib><creatorcontrib>Jones, Michael D.</creatorcontrib><creatorcontrib>Dalton, Emma</creatorcontrib><creatorcontrib>Holden, David W.</creatorcontrib><title>Simultaneous Identification of Bacterial Virulence Genes by Negative Selection</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteria, Pathogenic</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Diagnostic bacteriology</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA Transposable Elements</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. 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The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>7618105</pmid><doi>10.1126/science.7618105</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1995-07, Vol.269 (5222), p.400-403 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743453553 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Animals Bacteria Bacteria, Pathogenic Bacteriology Base Sequence Biological and medical sciences Diagnostic bacteriology DNA DNA probes DNA Transposable Elements Escherichia coli Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Genetic aspects Genetic screening Genetics Identification and classification Inoculum Mice Mice, Inbred BALB C Microbiology Molecular Sequence Data Mutagenesis, Insertional Mutation Nucleic Acid Hybridization Pathogenic bacteria Pathogenic microorganisms Polymerase Chain Reaction Salmonella Infections, Animal - microbiology Salmonella typhimurium - genetics Salmonella typhimurium - pathogenicity Screening Tests Sequence Tagged Sites Spleen Transcriptional regulatory elements Transposons Virulence Virulence (Microbiology) Virulence - genetics Viruses |
| title | Simultaneous Identification of Bacterial Virulence Genes by Negative Selection |
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