Refolding of partially and fully denatured lysozymes

Lysozyme refolding with high yields sometimes results from incomplete denaturation. Dithiothreitol (DTT) is a reductant commonly used to reduce and unfold disulfide-stabilized lysozymes. Through the use of fluorescence spectroscopy to access the extent of denaturation, we found that the rate and ext...

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Veröffentlicht in:	Biotechnology letters 2007-05, Vol.29 (5), p.723-729
Hauptverfasser:	Lin, Jiun-Liang, Ruaan, Ruoh-Chyu, Hsieh, Hsyue-Jen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Chickens denaturation disulfide bonds Dithiothreitol - pharmacology Fundamental and applied biological sciences. Psychology Glutathione Disulfide - metabolism lysozyme Muramidase - chemistry Muramidase - metabolism Phase transitions Protein Denaturation - drug effects Protein Folding Refolding Spectrometry, Fluorescence Urea - pharmacology
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