Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives
The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gene expression (TGE) is addressing this factor...
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| Veröffentlicht in: | Biotechnology letters 2007-05, Vol.29 (5), p.677-684 |
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| creator | Baldi, Lucia Hacker, David L Adam, Myriam Wurm, Florian M |
| description | The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gene expression (TGE) is addressing this factor in an impressive way: following DNA transfection, the production phase for TGE is usually 2-10 days. Recombinant proteins (r-proteins) produced by TGE can therefore enter the drug development and screening process in a very short time--weeks. With “classical” approaches to protein expression from mammalian cells, it takes months to establish a productive host cell line. This article summarizes efforts in industry and academia to use TGE to produce tens to hundreds of milligrams of r-proteins for either fundamental research or preclinical studies. |
| doi_str_mv | 10.1007/s10529-006-9297-y |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2007-05, Vol.29 (5), p.677-684 |
| issn | 0141-5492 1573-6776 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biological and medical sciences Biopharmaceuticals Biotechnology Biotechnology - methods Biotechnology - trends Clinical trials Drug development Fundamental and applied biological sciences. Psychology Gene Expression Gene Transfer Techniques Genetic Vectors Humans Mammalian cells Mammals Non-viral gene delivery Proteins Quality Recombinant protein Recombinant Proteins - biosynthesis Transfection Transient gene expression |
| title | Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives |
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