Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA
Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2002-05, Vol.296 (5572), p.1459-1462 |
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| creator | Srinivasan, Gayathri James, Carey M. Krzycki, Joseph A. |
| description | Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNACUAwith lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid. |
| doi_str_mv | 10.1126/science.1069588 |
| format | Article |
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Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNACUAwith lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1069588</identifier><identifier>PMID: 12029131</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Amino Acid Sequence ; Amino acids ; Amino Acyl-tRNA Synthetases - chemistry ; Amino Acyl-tRNA Synthetases - genetics ; Amino Acyl-tRNA Synthetases - metabolism ; Anticodon ; Archaeal Proteins ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Brackish ; Catalytic Domain ; Codon ; Codon, Terminator ; Codons ; Enzymes ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic translation ; Genetics ; Genomes ; Kinetics ; Lysine - analogs & derivatives ; Lysine - chemistry ; Lysine - genetics ; Lysine - metabolism ; Marine ; Methanosarcina barkeri ; Methanosarcina barkeri - chemistry ; Methanosarcina barkeri - enzymology ; Methanosarcina barkeri - genetics ; methylamine methyltransferase ; Methylamines ; Methyltransferases ; Methyltransferases - genetics ; Methyltransferases - metabolism ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Conformation ; Nucleic acids ; Protein Biosynthesis ; pylT gene ; pyrrolysine ; Recombinant Proteins - metabolism ; Ribonucleic acid ; RNA ; RNA, Archaeal - chemistry ; RNA, Archaeal - genetics ; RNA, Archaeal - metabolism ; RNA, Transfer - chemistry ; RNA, Transfer - genetics ; RNA, Transfer - metabolism ; Sequence Alignment ; Transcripts (Written Records) ; Transfer RNA ; Translation ; Translation (Genetics) ; Zoology</subject><ispartof>Science (American Association for the Advancement of Science), 2002-05, Vol.296 (5572), p.1459-1462</ispartof><rights>Copyright 2002 American Association for the Advancement of Science</rights><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2002 American Association for the Advancement of Science</rights><rights>COPYRIGHT 2002 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science May 24, 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c775t-70cd808a4c328856c15c5022373a6e91e4253aa26e22f5865f34edab64f907253</citedby><cites>FETCH-LOGICAL-c775t-70cd808a4c328856c15c5022373a6e91e4253aa26e22f5865f34edab64f907253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3076758$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3076758$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13698009$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12029131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Srinivasan, Gayathri</creatorcontrib><creatorcontrib>James, Carey M.</creatorcontrib><creatorcontrib>Krzycki, Joseph A.</creatorcontrib><title>Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNACUAwith lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Amino Acyl-tRNA Synthetases - chemistry</subject><subject>Amino Acyl-tRNA Synthetases - genetics</subject><subject>Amino Acyl-tRNA Synthetases - metabolism</subject><subject>Anticodon</subject><subject>Archaeal Proteins</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brackish</subject><subject>Catalytic Domain</subject><subject>Codon</subject><subject>Codon, Terminator</subject><subject>Codons</subject><subject>Enzymes</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic translation</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Kinetics</subject><subject>Lysine - analogs & derivatives</subject><subject>Lysine - chemistry</subject><subject>Lysine - genetics</subject><subject>Lysine - metabolism</subject><subject>Marine</subject><subject>Methanosarcina barkeri</subject><subject>Methanosarcina barkeri - chemistry</subject><subject>Methanosarcina barkeri - enzymology</subject><subject>Methanosarcina barkeri - genetics</subject><subject>methylamine methyltransferase</subject><subject>Methylamines</subject><subject>Methyltransferases</subject><subject>Methyltransferases - genetics</subject><subject>Methyltransferases - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>Protein Biosynthesis</subject><subject>pylT gene</subject><subject>pyrrolysine</subject><subject>Recombinant Proteins - 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chemistry</topic><topic>Amino Acyl-tRNA Synthetases - genetics</topic><topic>Amino Acyl-tRNA Synthetases - metabolism</topic><topic>Anticodon</topic><topic>Archaeal Proteins</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Brackish</topic><topic>Catalytic Domain</topic><topic>Codon</topic><topic>Codon, Terminator</topic><topic>Codons</topic><topic>Enzymes</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic translation</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Kinetics</topic><topic>Lysine - analogs & derivatives</topic><topic>Lysine - chemistry</topic><topic>Lysine - genetics</topic><topic>Lysine - metabolism</topic><topic>Marine</topic><topic>Methanosarcina barkeri</topic><topic>Methanosarcina barkeri - chemistry</topic><topic>Methanosarcina barkeri - enzymology</topic><topic>Methanosarcina barkeri - genetics</topic><topic>methylamine methyltransferase</topic><topic>Methylamines</topic><topic>Methyltransferases</topic><topic>Methyltransferases - genetics</topic><topic>Methyltransferases - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic acids</topic><topic>Protein Biosynthesis</topic><topic>pylT gene</topic><topic>pyrrolysine</topic><topic>Recombinant Proteins - metabolism</topic><topic>Ribonucleic 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Science)</jtitle><addtitle>Science</addtitle><date>2002-05-24</date><risdate>2002</risdate><volume>296</volume><issue>5572</issue><spage>1459</spage><epage>1462</epage><pages>1459-1462</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNACUAwith lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>12029131</pmid><doi>10.1126/science.1069588</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2002-05, Vol.296 (5572), p.1459-1462 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743407824 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Amino Acid Sequence Amino acids Amino Acyl-tRNA Synthetases - chemistry Amino Acyl-tRNA Synthetases - genetics Amino Acyl-tRNA Synthetases - metabolism Anticodon Archaeal Proteins Bacteriology Base Sequence Biological and medical sciences Brackish Catalytic Domain Codon Codon, Terminator Codons Enzymes Freshwater Fundamental and applied biological sciences. Psychology Genes Genetic translation Genetics Genomes Kinetics Lysine - analogs & derivatives Lysine - chemistry Lysine - genetics Lysine - metabolism Marine Methanosarcina barkeri Methanosarcina barkeri - chemistry Methanosarcina barkeri - enzymology Methanosarcina barkeri - genetics methylamine methyltransferase Methylamines Methyltransferases Methyltransferases - genetics Methyltransferases - metabolism Microbiology Molecular Sequence Data Nucleic Acid Conformation Nucleic acids Protein Biosynthesis pylT gene pyrrolysine Recombinant Proteins - metabolism Ribonucleic acid RNA RNA, Archaeal - chemistry RNA, Archaeal - genetics RNA, Archaeal - metabolism RNA, Transfer - chemistry RNA, Transfer - genetics RNA, Transfer - metabolism Sequence Alignment Transcripts (Written Records) Transfer RNA Translation Translation (Genetics) Zoology |
| title | Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T03%3A15%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pyrrolysine%20Encoded%20by%20UAG%20in%20Archaea:%20Charging%20of%20a%20UAG-Decoding%20Specialized%20tRNA&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Srinivasan,%20Gayathri&rft.date=2002-05-24&rft.volume=296&rft.issue=5572&rft.spage=1459&rft.epage=1462&rft.pages=1459-1462&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.1069588&rft_dat=%3Cgale_proqu%3EA87105413%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213573580&rft_id=info:pmid/12029131&rft_galeid=A87105413&rft_jstor_id=3076758&rfr_iscdi=true |