Peroxiredoxin Evolution and the Regulation of Hydrogen Peroxide Signaling
Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motif...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2003-04, Vol.300 (5619), p.650-653 |
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| creator | Wood, Zachary A. Poole, Leslie B. Karplus, P. Andrew |
| description | Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction. |
| doi_str_mv | 10.1126/science.1080405 |
| format | Article |
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Psychology ; Humans ; Hydrogen ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Models, Chemical ; Models, Molecular ; Molecular and cellular biology ; Molecular Sequence Data ; Observations ; Oxidation-Reduction ; Peroxidases - chemistry ; Peroxidases - metabolism ; Peroxides ; Peroxiredoxins ; Physiological regulation ; Protein Conformation ; Protein Folding ; Protein Structure, Secondary ; Radiology ; Salmonella typhimurium - enzymology ; Sequence Alignment ; Signal Transduction ; Signaling ; Signals and signaling ; Sulfenic Acids - metabolism ; Sulfinic Acids - metabolism ; Transduction ; Transduction (Genetics) ; Yeast fungi ; Yeasts ; Yeasts (Fungi) ; Yeasts - enzymology</subject><ispartof>Science (American Association for the Advancement of Science), 2003-04, Vol.300 (5619), p.650-653</ispartof><rights>Copyright 2003 American Association for the Advancement of Science</rights><rights>2003 INIST-CNRS</rights><rights>COPYRIGHT 2003 American Association for the Advancement of Science</rights><rights>COPYRIGHT 2003 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Apr 25, 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c750t-ce7ca78cbb929140e15e5b5d34b3f3d3c70c15ad72153702652cdef129e525813</citedby><cites>FETCH-LOGICAL-c750t-ce7ca78cbb929140e15e5b5d34b3f3d3c70c15ad72153702652cdef129e525813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3834367$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3834367$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14771592$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12714747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wood, Zachary A.</creatorcontrib><creatorcontrib>Poole, Leslie B.</creatorcontrib><creatorcontrib>Karplus, P. Andrew</creatorcontrib><title>Peroxiredoxin Evolution and the Regulation of Hydrogen Peroxide Signaling</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.</description><subject>Active sites</subject><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Antioxidants</subject><subject>Antioxidants (Nutrients)</subject><subject>Bacteria</subject><subject>Bacteria - enzymology</subject><subject>Binding Sites</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Catalysis</subject><subject>Chemical properties</subject><subject>Control</subject><subject>Crystallography</subject><subject>Crystallography, X-Ray</subject><subject>Cysteine - metabolism</subject><subject>Disulfides</subject><subject>Disulfides - chemistry</subject><subject>Disulfides - metabolism</subject><subject>Enzymes</subject><subject>Eukaryotes</subject><subject>Evolution</subject><subject>Evolution, Molecular</subject><subject>Fundamental and applied biological sciences. 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We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>12714747</pmid><doi>10.1126/science.1080405</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Science (American Association for the Advancement of Science), 2003-04, Vol.300 (5619), p.650-653 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | MEDLINE; Science Magazine; JSTOR Archive Collection A-Z Listing |
| subjects | Active sites Amino Acid Motifs Amino Acid Sequence Antioxidants Antioxidants (Nutrients) Bacteria Bacteria - enzymology Binding Sites Biochemistry Biological and medical sciences Catalysis Chemical properties Control Crystallography Crystallography, X-Ray Cysteine - metabolism Disulfides Disulfides - chemistry Disulfides - metabolism Enzymes Eukaryotes Evolution Evolution, Molecular Fundamental and applied biological sciences. Psychology Humans Hydrogen Hydrogen peroxide Hydrogen Peroxide - metabolism Models, Chemical Models, Molecular Molecular and cellular biology Molecular Sequence Data Observations Oxidation-Reduction Peroxidases - chemistry Peroxidases - metabolism Peroxides Peroxiredoxins Physiological regulation Protein Conformation Protein Folding Protein Structure, Secondary Radiology Salmonella typhimurium - enzymology Sequence Alignment Signal Transduction Signaling Signals and signaling Sulfenic Acids - metabolism Sulfinic Acids - metabolism Transduction Transduction (Genetics) Yeast fungi Yeasts Yeasts (Fungi) Yeasts - enzymology |
| title | Peroxiredoxin Evolution and the Regulation of Hydrogen Peroxide Signaling |
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