Production of d-tagatose at high temperatures using immobilized Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t ₁/₂ = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l,...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology letters 2007-04, Vol.29 (4), p.569-574
Hauptverfasser:	Hong, Young-Ho, Lee, Dong-Woo, Lee, Sang-Jae, Choe, Eun-Ah, Kim, Seong-Bo, Lee, Yoon-Hee, Cheigh, Chan-Ick, Pyun, Yu-Ryang
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabinose - genetics Arabinose - metabolism Bacteria Bacteriology Biological and medical sciences Biotechnology Cell Culture Techniques - methods d-Tagatose E coli Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Esherichia coli Fundamental and applied biological sciences. Psychology Hexoses - biosynthesis Hexoses - genetics High temperature Immobilization Isomerases - genetics Isomerases - metabolism l-Arabinose isomerase Protein Engineering - methods Recombinant Proteins - metabolism Sweetening Agents - metabolism Temperature Thermotoga neapolitana Thermotoga neapolitana - enzymology Thermotoga neapolitana - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t ₁/₂ = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/s10529-006-9277-2