Rapid purification of plasmid DNAs by hydroxyapatite chromatography [yeast extract, Escherichia coli strains and plasmids]

A method is described for the rapid preparation of plasmid DNAs of molecular weight up to 14 × 106. This method involves the chromatography, at room temperature, of bacterial cleared lysates on hydroxyapatite in the presence of high concentrations of phosphate and urea. All detectable protein and RN...

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Veröffentlicht in:	European journal of biochemistry 1978-11, Vol.91 (1), p.303-310
Hauptverfasser:	Colman, A, Byers, M.J, Primrose, S.B, Lyons, A. (Warwick Univ. (UK). Dept. of Biological Science)
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography - methods DNA, Bacterial - isolation & purification Escherichia coli - analysis Hydroxyapatites Molecular Weight Nucleic Acid Denaturation Plasmids Species Specificity
Online-Zugang:	Volltext
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