Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice
Animal donors such as pigs could provide an alternative source of organs for transplantation. However, the promise of xenotransplantation is offset by the possible public health risk of a cross-species infection 1 , 2 . All pigs contain several copies of porcine endogenous retroviruses (PERV) 3 , 4...
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| Veröffentlicht in: | Nature (London) 2000-09, Vol.407 (6800), p.90-94 |
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| creator | van der Laan, Luc J.W. Lockey, Christopher Griffeth, Bradley C. Frasier, Francine S. Wilson, Carolyn A. Onions, David E. Hering, Bernhard J. Long, Zhifeng Otto, Edward Torbett, Bruce E. Salomon, Daniel R. |
| description | Animal donors such as pigs could provide an alternative source of organs for transplantation. However, the promise of xenotransplantation is offset by the possible public health risk of a cross-species infection
1
,
2
. All pigs contain several copies of porcine endogenous retroviruses (PERV)
3
,
4
, and at least three variants of PERV can infect human cell lines
in vitro
in co-culture, infectivity and pseudotyping experiments
3
,
5
,
6
,
7
. Thus, if xenotransplantation of pig tissues results in PERV viral replication, there is a risk of spreading and adaptation of this retrovirus to the human host. C-type retroviruses related to PERV are associated with malignancies of haematopoietic lineage cells in their natural hosts
8
. Here we show that pig pancreatic islets produce PERV and can infect human cells in culture. After transplantation into NOD/SCID (non-obese diabetic, severe combined immunodeficiency) mice, we detect ongoing viral expression and several tissue compartments become infected. This is the first evidence that PERV is transcriptionally active and infectious cross-species
in vivo
after transplantation of pig tissues. These results show that a concern for PERV infection risk associated with pig islet xenotransplantation in immunosuppressed human patients may be justified. |
| doi_str_mv | 10.1038/35024089 |
| format | Article |
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1
,
2
. All pigs contain several copies of porcine endogenous retroviruses (PERV)
3
,
4
, and at least three variants of PERV can infect human cell lines
in vitro
in co-culture, infectivity and pseudotyping experiments
3
,
5
,
6
,
7
. Thus, if xenotransplantation of pig tissues results in PERV viral replication, there is a risk of spreading and adaptation of this retrovirus to the human host. C-type retroviruses related to PERV are associated with malignancies of haematopoietic lineage cells in their natural hosts
8
. Here we show that pig pancreatic islets produce PERV and can infect human cells in culture. After transplantation into NOD/SCID (non-obese diabetic, severe combined immunodeficiency) mice, we detect ongoing viral expression and several tissue compartments become infected. This is the first evidence that PERV is transcriptionally active and infectious cross-species
in vivo
after transplantation of pig tissues. These results show that a concern for PERV infection risk associated with pig islet xenotransplantation in immunosuppressed human patients may be justified.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/35024089</identifier><identifier>PMID: 10993079</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biological and medical sciences ; Cells ; Cells, Cultured ; Endogenous Retroviruses ; Experimental viral diseases and models ; Health risks ; Hogs ; Humanities and Social Sciences ; Humans ; Infections ; Infectious diseases ; Islets of Langerhans - virology ; letter ; Medical sciences ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Molecular Sequence Data ; multidisciplinary ; Pancreas Transplantation - adverse effects ; Porcine endogenous retrovirus ; Public health ; Retroviridae Infections - etiology ; Retroviridae Infections - transmission ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; RNA, Viral - analysis ; Rodents ; Science ; Science (multidisciplinary) ; Species Specificity ; Swine ; Swine - virology ; Transplantation ; Transplantation Chimera ; Transplantation, Heterologous - adverse effects ; Viral diseases ; Viruses ; Xenotransplantation</subject><ispartof>Nature (London), 2000-09, Vol.407 (6800), p.90-94</ispartof><rights>Macmillan Magazines Ltd. 2000</rights><rights>2000 INIST-CNRS</rights><rights>COPYRIGHT 2000 Nature Publishing Group</rights><rights>Copyright Macmillan Journals Ltd. Sep 7, 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c705t-95544e7bc486c5b245b36aa81d37427e2f3f4c573e206e0af8e8537f588c90d23</citedby><cites>FETCH-LOGICAL-c705t-95544e7bc486c5b245b36aa81d37427e2f3f4c573e206e0af8e8537f588c90d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/35024089$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/35024089$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1465794$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10993079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Laan, Luc J.W.</creatorcontrib><creatorcontrib>Lockey, Christopher</creatorcontrib><creatorcontrib>Griffeth, Bradley C.</creatorcontrib><creatorcontrib>Frasier, Francine S.</creatorcontrib><creatorcontrib>Wilson, Carolyn A.</creatorcontrib><creatorcontrib>Onions, David E.</creatorcontrib><creatorcontrib>Hering, Bernhard J.</creatorcontrib><creatorcontrib>Long, Zhifeng</creatorcontrib><creatorcontrib>Otto, Edward</creatorcontrib><creatorcontrib>Torbett, Bruce E.</creatorcontrib><creatorcontrib>Salomon, Daniel R.</creatorcontrib><title>Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Animal donors such as pigs could provide an alternative source of organs for transplantation. However, the promise of xenotransplantation is offset by the possible public health risk of a cross-species infection
1
,
2
. All pigs contain several copies of porcine endogenous retroviruses (PERV)
3
,
4
, and at least three variants of PERV can infect human cell lines
in vitro
in co-culture, infectivity and pseudotyping experiments
3
,
5
,
6
,
7
. Thus, if xenotransplantation of pig tissues results in PERV viral replication, there is a risk of spreading and adaptation of this retrovirus to the human host. C-type retroviruses related to PERV are associated with malignancies of haematopoietic lineage cells in their natural hosts
8
. Here we show that pig pancreatic islets produce PERV and can infect human cells in culture. After transplantation into NOD/SCID (non-obese diabetic, severe combined immunodeficiency) mice, we detect ongoing viral expression and several tissue compartments become infected. This is the first evidence that PERV is transcriptionally active and infectious cross-species
in vivo
after transplantation of pig tissues. These results show that a concern for PERV infection risk associated with pig islet xenotransplantation in immunosuppressed human patients may be justified.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Endogenous Retroviruses</subject><subject>Experimental viral diseases and models</subject><subject>Health risks</subject><subject>Hogs</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Islets of Langerhans - virology</subject><subject>letter</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Molecular Sequence Data</subject><subject>multidisciplinary</subject><subject>Pancreas Transplantation - adverse effects</subject><subject>Porcine endogenous retrovirus</subject><subject>Public health</subject><subject>Retroviridae Infections - etiology</subject><subject>Retroviridae Infections - transmission</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Viral - analysis</subject><subject>Rodents</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Species Specificity</subject><subject>Swine</subject><subject>Swine - virology</subject><subject>Transplantation</subject><subject>Transplantation Chimera</subject><subject>Transplantation, Heterologous - adverse effects</subject><subject>Viral diseases</subject><subject>Viruses</subject><subject>Xenotransplantation</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0mtv0zAUBmALgdgoSPwCFCHERSjj-O58rMqt0gQSG9rHyHWPK0-p09kp2v49Hi3qCmMoHxLFT944bw4hTykcUeDmHZfABJjmHjmkQqtaKKPvk0MAZmowXB2QRzmfA4CkWjwkBxSahoNuDsnZNHp0Q-hjNbuqVn1yIWKFcd4vMPbrXCUcUv8jpHJp_YCpCrnDobosq0OyMa86Gwf7KyDE6mQyfV8tg8PH5IG3XcYn2_OIfP_44XTyuT7--mk6GR_XToMc6kZKIVDPnDDKyRkTcsaVtYbOuRZMI_PcCyc1RwYKwXqDRnLtpTGugTnjI_Jqk7tK_cUa89AuQ3bYlV1h2X6rBeeMaaBFvrxbMqYaKHWOyOs7IdVaKSG0gP9mUq0YN0wU-PwPeN6vUyzNtAxKVgNSFVRv0MJ22Iborxt25T9gsl0f0Ydye0yNAaUbbnahe96twkV7Ex3dgsoxx_Kfbk19s_dAMQNeDgu7zrmdnnzbt2__bcenZ5Mv-3pbrEt9zgl9u0phadNVS6G9nuP29xwX-mzb13q2xPkNuBncAl5sgc3Odr6Mogt554SSuhG7j8llJS4w7Xr_650_AfvWAHk</recordid><startdate>20000907</startdate><enddate>20000907</enddate><creator>van 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by porcine endogenous retrovirus after islet xenotransplantation in SCID mice</title><author>van der Laan, Luc J.W. ; Lockey, Christopher ; Griffeth, Bradley C. ; Frasier, Francine S. ; Wilson, Carolyn A. ; Onions, David E. ; Hering, Bernhard J. ; Long, Zhifeng ; Otto, Edward ; Torbett, Bruce E. ; Salomon, Daniel R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c705t-95544e7bc486c5b245b36aa81d37427e2f3f4c573e206e0af8e8537f588c90d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Endogenous Retroviruses</topic><topic>Experimental viral diseases and models</topic><topic>Health risks</topic><topic>Hogs</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Islets of Langerhans - virology</topic><topic>letter</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Molecular Sequence Data</topic><topic>multidisciplinary</topic><topic>Pancreas Transplantation - adverse effects</topic><topic>Porcine endogenous retrovirus</topic><topic>Public health</topic><topic>Retroviridae Infections - etiology</topic><topic>Retroviridae Infections - transmission</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Viral - analysis</topic><topic>Rodents</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Species Specificity</topic><topic>Swine</topic><topic>Swine - virology</topic><topic>Transplantation</topic><topic>Transplantation Chimera</topic><topic>Transplantation, Heterologous - adverse effects</topic><topic>Viral diseases</topic><topic>Viruses</topic><topic>Xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Laan, Luc J.W.</creatorcontrib><creatorcontrib>Lockey, Christopher</creatorcontrib><creatorcontrib>Griffeth, Bradley C.</creatorcontrib><creatorcontrib>Frasier, Francine S.</creatorcontrib><creatorcontrib>Wilson, Carolyn A.</creatorcontrib><creatorcontrib>Onions, David E.</creatorcontrib><creatorcontrib>Hering, Bernhard J.</creatorcontrib><creatorcontrib>Long, Zhifeng</creatorcontrib><creatorcontrib>Otto, Edward</creatorcontrib><creatorcontrib>Torbett, Bruce E.</creatorcontrib><creatorcontrib>Salomon, Daniel R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: 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and Information Systems Abstracts Professional</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Laan, Luc J.W.</au><au>Lockey, Christopher</au><au>Griffeth, Bradley C.</au><au>Frasier, Francine S.</au><au>Wilson, Carolyn A.</au><au>Onions, David E.</au><au>Hering, Bernhard J.</au><au>Long, Zhifeng</au><au>Otto, Edward</au><au>Torbett, Bruce E.</au><au>Salomon, Daniel R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2000-09-07</date><risdate>2000</risdate><volume>407</volume><issue>6800</issue><spage>90</spage><epage>94</epage><pages>90-94</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Animal donors such as pigs could provide an alternative source of organs for transplantation. However, the promise of xenotransplantation is offset by the possible public health risk of a cross-species infection
1
,
2
. All pigs contain several copies of porcine endogenous retroviruses (PERV)
3
,
4
, and at least three variants of PERV can infect human cell lines
in vitro
in co-culture, infectivity and pseudotyping experiments
3
,
5
,
6
,
7
. Thus, if xenotransplantation of pig tissues results in PERV viral replication, there is a risk of spreading and adaptation of this retrovirus to the human host. C-type retroviruses related to PERV are associated with malignancies of haematopoietic lineage cells in their natural hosts
8
. Here we show that pig pancreatic islets produce PERV and can infect human cells in culture. After transplantation into NOD/SCID (non-obese diabetic, severe combined immunodeficiency) mice, we detect ongoing viral expression and several tissue compartments become infected. This is the first evidence that PERV is transcriptionally active and infectious cross-species
in vivo
after transplantation of pig tissues. These results show that a concern for PERV infection risk associated with pig islet xenotransplantation in immunosuppressed human patients may be justified.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>10993079</pmid><doi>10.1038/35024089</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 2000-09, Vol.407 (6800), p.90-94 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743322701 |
| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Cells Cells, Cultured Endogenous Retroviruses Experimental viral diseases and models Health risks Hogs Humanities and Social Sciences Humans Infections Infectious diseases Islets of Langerhans - virology letter Medical sciences Mice Mice, Inbred NOD Mice, SCID Molecular Sequence Data multidisciplinary Pancreas Transplantation - adverse effects Porcine endogenous retrovirus Public health Retroviridae Infections - etiology Retroviridae Infections - transmission Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis RNA, Viral - analysis Rodents Science Science (multidisciplinary) Species Specificity Swine Swine - virology Transplantation Transplantation Chimera Transplantation, Heterologous - adverse effects Viral diseases Viruses Xenotransplantation |
| title | Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T22%3A05%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Infection%20by%20porcine%20endogenous%20retrovirus%20after%20islet%20xenotransplantation%20in%20SCID%20mice&rft.jtitle=Nature%20(London)&rft.au=van%20der%20Laan,%20Luc%20J.W.&rft.date=2000-09-07&rft.volume=407&rft.issue=6800&rft.spage=90&rft.epage=94&rft.pages=90-94&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/35024089&rft_dat=%3Cgale_proqu%3EA188067938%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204479056&rft_id=info:pmid/10993079&rft_galeid=A188067938&rfr_iscdi=true |