End-to-End Stacking and Liquid Crystal Condensation of 6- to 20-Base Pair DNA Duplexes
Short complementary B-form DNA oligomers, 6 to 20 base pairs in length, are found to exhibit nematic and columnar liquid crystal phases, even though such duplexes lack the shape anisotropy required for liquid crystal ordering. Structural study shows that these phases are produced by the end-to-end a...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2007-11, Vol.318 (5854), p.1276-1279 |
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| creator | Nakata, Michi Zanchetta, Giuliano Chapman, Brandon D Jones, Christopher D Cross, Julie O Pindak, Ronald Bellini, Tommaso Clark, Noel A |
| description | Short complementary B-form DNA oligomers, 6 to 20 base pairs in length, are found to exhibit nematic and columnar liquid crystal phases, even though such duplexes lack the shape anisotropy required for liquid crystal ordering. Structural study shows that these phases are produced by the end-to-end adhesion and consequent stacking of the duplex oligomers into polydisperse anisotropic rod-shaped aggregates, which can order into liquid crystals. Upon cooling mixed solutions of short DNA oligomers, in which only a small fraction of the DNA present is complementary, the duplex-forming oligomers phase-separate into liquid crystal droplets, leaving the unpaired single strands in isotropic solution. In a chemical environment where oligomer ligation is possible, such ordering and condensation would provide an autocatalytic link whereby complementarity promotes the extended polymerization of complementary oligomers. |
| doi_str_mv | 10.1126/science.1143826 |
| format | Article |
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(ANL), Argonne, IL (United States)</creatorcontrib><description>Short complementary B-form DNA oligomers, 6 to 20 base pairs in length, are found to exhibit nematic and columnar liquid crystal phases, even though such duplexes lack the shape anisotropy required for liquid crystal ordering. Structural study shows that these phases are produced by the end-to-end adhesion and consequent stacking of the duplex oligomers into polydisperse anisotropic rod-shaped aggregates, which can order into liquid crystals. Upon cooling mixed solutions of short DNA oligomers, in which only a small fraction of the DNA present is complementary, the duplex-forming oligomers phase-separate into liquid crystal droplets, leaving the unpaired single strands in isotropic solution. In a chemical environment where oligomer ligation is possible, such ordering and condensation would provide an autocatalytic link whereby complementarity promotes the extended polymerization of complementary oligomers.</description><identifier>ISSN: 0036-8075</identifier><identifier>ISSN: 0193-4511</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1143826</identifier><identifier>PMID: 18033877</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>ADHESION ; Aggregation ; Analytical, structural and metabolic biochemistry ; ANISOTROPY ; B form DNA ; Base Pairing ; BASIC BIOLOGICAL SCIENCES ; Biochemistry ; Biological and medical sciences ; Columnar crystals ; Condensation ; Crystallization ; Crystallography ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; Dna, deoxyribonucleoproteins ; Fundamental and applied biological sciences. 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(ANL), Argonne, IL (United States)</creatorcontrib><title>End-to-End Stacking and Liquid Crystal Condensation of 6- to 20-Base Pair DNA Duplexes</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Short complementary B-form DNA oligomers, 6 to 20 base pairs in length, are found to exhibit nematic and columnar liquid crystal phases, even though such duplexes lack the shape anisotropy required for liquid crystal ordering. Structural study shows that these phases are produced by the end-to-end adhesion and consequent stacking of the duplex oligomers into polydisperse anisotropic rod-shaped aggregates, which can order into liquid crystals. Upon cooling mixed solutions of short DNA oligomers, in which only a small fraction of the DNA present is complementary, the duplex-forming oligomers phase-separate into liquid crystal droplets, leaving the unpaired single strands in isotropic solution. In a chemical environment where oligomer ligation is possible, such ordering and condensation would provide an autocatalytic link whereby complementarity promotes the extended polymerization of complementary oligomers.</description><subject>ADHESION</subject><subject>Aggregation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANISOTROPY</subject><subject>B form DNA</subject><subject>Base Pairing</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Columnar crystals</subject><subject>Condensation</subject><subject>Crystallization</subject><subject>Crystallography</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Fundamental and applied biological sciences. 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(ANL), Argonne, IL (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>End-to-End Stacking and Liquid Crystal Condensation of 6- to 20-Base Pair DNA Duplexes</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2007-11-23</date><risdate>2007</risdate><volume>318</volume><issue>5854</issue><spage>1276</spage><epage>1279</epage><pages>1276-1279</pages><issn>0036-8075</issn><issn>0193-4511</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Short complementary B-form DNA oligomers, 6 to 20 base pairs in length, are found to exhibit nematic and columnar liquid crystal phases, even though such duplexes lack the shape anisotropy required for liquid crystal ordering. Structural study shows that these phases are produced by the end-to-end adhesion and consequent stacking of the duplex oligomers into polydisperse anisotropic rod-shaped aggregates, which can order into liquid crystals. Upon cooling mixed solutions of short DNA oligomers, in which only a small fraction of the DNA present is complementary, the duplex-forming oligomers phase-separate into liquid crystal droplets, leaving the unpaired single strands in isotropic solution. In a chemical environment where oligomer ligation is possible, such ordering and condensation would provide an autocatalytic link whereby complementarity promotes the extended polymerization of complementary oligomers.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>18033877</pmid><doi>10.1126/science.1143826</doi><tpages>4</tpages></addata></record> |
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| ispartof | Science (American Association for the Advancement of Science), 2007-11, Vol.318 (5854), p.1276-1279 |
| issn | 0036-8075 0193-4511 1095-9203 |
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| source | Jstor Complete Legacy; MEDLINE; Science Magazine |
| subjects | ADHESION Aggregation Analytical, structural and metabolic biochemistry ANISOTROPY B form DNA Base Pairing BASIC BIOLOGICAL SCIENCES Biochemistry Biological and medical sciences Columnar crystals Condensation Crystallization Crystallography Deoxyribonucleic acid DNA DNA - chemistry Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Grants Ligation Liquid crystal polymers LIQUID CRYSTALS Molecular biology Nucleic Acid Conformation Nucleic acids Oligodeoxyribonucleotides - chemistry Oligomers Phase diagrams Phase Transition POLYMERIZATION SHAPE Solutes Temperature Thermodynamics X-Ray Diffraction |
| title | End-to-End Stacking and Liquid Crystal Condensation of 6- to 20-Base Pair DNA Duplexes |
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