Methanotrophy below pH 1 by a new Verrucomicrobia species
Passing the acid test Bacteria that consume the greenhouse gas methane are potentially important players in the atmospheric budget, with the potential to sop up methane from the Earth's crust that would otherwise contribute to the atmospheric budget. Two new methane-utilizing bacteria have been...
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| Veröffentlicht in: | Nature 2007-12, Vol.450 (7171), p.874-878 |
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| creator | Pol, Arjan Heijmans, Klaas Harhangi, Harry R. Tedesco, Dario Jetten, Mike S. M. Op den Camp, Huub J. M. |
| description | Passing the acid test
Bacteria that consume the greenhouse gas methane are potentially important players in the atmospheric budget, with the potential to sop up methane from the Earth's crust that would otherwise contribute to the atmospheric budget. Two new methane-utilizing bacteria have been isolated independently and both break new ground: unlike previous methanotrophic isolates, which are proteobacteria, they belong to the widely distributed Verrucomicrobia phylum. And both isolates display optimum growth and methane oxidation in remarkably acidic conditions, at pHs as low as 0.8 to 2.5.
Acidimethylosilex fumarolicum
SolV was isolated from a fuming vent on the Solfatara volcano near Naples, Italy, and
Methylokorus infernorum
from hot soil in the Hell's Gate (Tikitere) geothermal area of New Zealand.
The isolation of the acidophilic bacterium
Acidimethylosilex fumarolicum
SolV from a fumarole is described. Unlike all previous methanotrophic isolates, which belong to the Alpha- or Gammaproteobacteria, it belongs to the widely distributed Verrumicrobia.
Mud volcanoes, mudpots and fumaroles are remarkable geological features characterized by the emission of gas, water and/or semi-liquid mud matrices
1
with significant methane fluxes to the atmosphere (10
-1
to 10
3
t y
-1
)
2
,
3
,
4
. Environmental conditions in these areas vary from ambient temperature and neutral pH to high temperatures and low pH. Although there are strong indications for biological methane consumption in mud volcanoes
4
,
5
, no methanotrophic bacteria are known that would thrive in the hostile conditions of fumaroles (temperatures up to 70 °C and pH down to 1.8)
2
. The first step in aerobic methane oxidation is performed by a soluble or membrane-bound methane mono-oxygenase. Here we report that
pmoA
(encoding the β-subunit of membrane-bound methane mono-oxygenase) clone libraries, made by using DNA extracted from the Solfatara volcano mudpot and surrounding bare soil near the fumaroles, showed clusters of novel and distant
pmoA
genes. After methanotrophic enrichment at 50 °C and pH 2.0 the most distant cluster, sharing less than 50% identity with any other described
pmoA
gene, was represented in the culture. Finally we isolated an acidiphilic methanotrophic bacterium
Acidimethylosilex fumarolicum
SolV belonging to the Planctomycetes/Verrucomicrobia/Chlamydiae superphylum
6
, ‘outside’ the subphyla of the Alpha- and Gammaproteobacteria containing the established methanotrophs. This |
| doi_str_mv | 10.1038/nature06222 |
| format | Article |
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Bacteria that consume the greenhouse gas methane are potentially important players in the atmospheric budget, with the potential to sop up methane from the Earth's crust that would otherwise contribute to the atmospheric budget. Two new methane-utilizing bacteria have been isolated independently and both break new ground: unlike previous methanotrophic isolates, which are proteobacteria, they belong to the widely distributed Verrucomicrobia phylum. And both isolates display optimum growth and methane oxidation in remarkably acidic conditions, at pHs as low as 0.8 to 2.5.
Acidimethylosilex fumarolicum
SolV was isolated from a fuming vent on the Solfatara volcano near Naples, Italy, and
Methylokorus infernorum
from hot soil in the Hell's Gate (Tikitere) geothermal area of New Zealand.
The isolation of the acidophilic bacterium
Acidimethylosilex fumarolicum
SolV from a fumarole is described. Unlike all previous methanotrophic isolates, which belong to the Alpha- or Gammaproteobacteria, it belongs to the widely distributed Verrumicrobia.
Mud volcanoes, mudpots and fumaroles are remarkable geological features characterized by the emission of gas, water and/or semi-liquid mud matrices
1
with significant methane fluxes to the atmosphere (10
-1
to 10
3
t y
-1
)
2
,
3
,
4
. Environmental conditions in these areas vary from ambient temperature and neutral pH to high temperatures and low pH. Although there are strong indications for biological methane consumption in mud volcanoes
4
,
5
, no methanotrophic bacteria are known that would thrive in the hostile conditions of fumaroles (temperatures up to 70 °C and pH down to 1.8)
2
. The first step in aerobic methane oxidation is performed by a soluble or membrane-bound methane mono-oxygenase. Here we report that
pmoA
(encoding the β-subunit of membrane-bound methane mono-oxygenase) clone libraries, made by using DNA extracted from the Solfatara volcano mudpot and surrounding bare soil near the fumaroles, showed clusters of novel and distant
pmoA
genes. After methanotrophic enrichment at 50 °C and pH 2.0 the most distant cluster, sharing less than 50% identity with any other described
pmoA
gene, was represented in the culture. Finally we isolated an acidiphilic methanotrophic bacterium
Acidimethylosilex fumarolicum
SolV belonging to the Planctomycetes/Verrucomicrobia/Chlamydiae superphylum
6
, ‘outside’ the subphyla of the Alpha- and Gammaproteobacteria containing the established methanotrophs. This bacterium grows under oxygen limitation on methane as the sole source of energy, down to pH 0.8—far below the pH optimum of any previously described methanotroph.
A. fumarolicum
SolV has three different
pmoA
genes, with two that are very similar to sequences retrieved from the mudpot. Highly homologous environmental 16S rRNA gene sequences from Yellowstone Park show that this new type of methanotrophic bacteria may be a common inhabitant of extreme environments. This is the first time that a representative of the widely distributed Verrucomicrobia phylum, of which most members remain uncultivated
6
, is coupled to a geochemically relevant reaction.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/nature06222</identifier><identifier>PMID: 18004305</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Acids - analysis ; Acids - chemistry ; Ambient temperature ; Atmosphere ; Bacteria ; Bacteria - classification ; Bacteria - enzymology ; Bacteria - genetics ; Bacteria - metabolism ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Clusters ; Deoxyribonucleic acid ; DNA ; Emissions ; Environmental conditions ; Fumaroles ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genes ; Genes, Bacterial - genetics ; Genetics ; Geologic Sediments - microbiology ; Geology ; High temperature ; Hot Springs - microbiology ; Hot Temperature ; Humanities and Social Sciences ; Hydrogen-Ion Concentration ; In Situ Hybridization, Fluorescence ; letter ; Membranes ; Methane ; Methane - chemistry ; Methane - metabolism ; Microbiology ; Molecular Sequence Data ; Mud ; multidisciplinary ; Oxidation ; Oxygen - analysis ; Oxygenases - genetics ; Phylogeny ; Planctomycetes ; RNA, Ribosomal, 16S - genetics ; Science ; Science (multidisciplinary) ; Verrucomicrobia ; Volcanic Eruptions ; Volcanoes</subject><ispartof>Nature, 2007-12, Vol.450 (7171), p.874-878</ispartof><rights>Springer Nature Limited 2007</rights><rights>2008 INIST-CNRS</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Dec 6, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c8322-eb27a559821619c28c5fda0866c73a8568695e84a4be45db4e7bf0cfbe5b016a3</citedby><cites>FETCH-LOGICAL-c8322-eb27a559821619c28c5fda0866c73a8568695e84a4be45db4e7bf0cfbe5b016a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nature06222$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nature06222$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19868809$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18004305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pol, Arjan</creatorcontrib><creatorcontrib>Heijmans, Klaas</creatorcontrib><creatorcontrib>Harhangi, Harry R.</creatorcontrib><creatorcontrib>Tedesco, Dario</creatorcontrib><creatorcontrib>Jetten, Mike S. M.</creatorcontrib><creatorcontrib>Op den Camp, Huub J. M.</creatorcontrib><title>Methanotrophy below pH 1 by a new Verrucomicrobia species</title><title>Nature</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Passing the acid test
Bacteria that consume the greenhouse gas methane are potentially important players in the atmospheric budget, with the potential to sop up methane from the Earth's crust that would otherwise contribute to the atmospheric budget. Two new methane-utilizing bacteria have been isolated independently and both break new ground: unlike previous methanotrophic isolates, which are proteobacteria, they belong to the widely distributed Verrucomicrobia phylum. And both isolates display optimum growth and methane oxidation in remarkably acidic conditions, at pHs as low as 0.8 to 2.5.
Acidimethylosilex fumarolicum
SolV was isolated from a fuming vent on the Solfatara volcano near Naples, Italy, and
Methylokorus infernorum
from hot soil in the Hell's Gate (Tikitere) geothermal area of New Zealand.
The isolation of the acidophilic bacterium
Acidimethylosilex fumarolicum
SolV from a fumarole is described. Unlike all previous methanotrophic isolates, which belong to the Alpha- or Gammaproteobacteria, it belongs to the widely distributed Verrumicrobia.
Mud volcanoes, mudpots and fumaroles are remarkable geological features characterized by the emission of gas, water and/or semi-liquid mud matrices
1
with significant methane fluxes to the atmosphere (10
-1
to 10
3
t y
-1
)
2
,
3
,
4
. Environmental conditions in these areas vary from ambient temperature and neutral pH to high temperatures and low pH. Although there are strong indications for biological methane consumption in mud volcanoes
4
,
5
, no methanotrophic bacteria are known that would thrive in the hostile conditions of fumaroles (temperatures up to 70 °C and pH down to 1.8)
2
. The first step in aerobic methane oxidation is performed by a soluble or membrane-bound methane mono-oxygenase. Here we report that
pmoA
(encoding the β-subunit of membrane-bound methane mono-oxygenase) clone libraries, made by using DNA extracted from the Solfatara volcano mudpot and surrounding bare soil near the fumaroles, showed clusters of novel and distant
pmoA
genes. After methanotrophic enrichment at 50 °C and pH 2.0 the most distant cluster, sharing less than 50% identity with any other described
pmoA
gene, was represented in the culture. Finally we isolated an acidiphilic methanotrophic bacterium
Acidimethylosilex fumarolicum
SolV belonging to the Planctomycetes/Verrucomicrobia/Chlamydiae superphylum
6
, ‘outside’ the subphyla of the Alpha- and Gammaproteobacteria containing the established methanotrophs. This bacterium grows under oxygen limitation on methane as the sole source of energy, down to pH 0.8—far below the pH optimum of any previously described methanotroph.
A. fumarolicum
SolV has three different
pmoA
genes, with two that are very similar to sequences retrieved from the mudpot. Highly homologous environmental 16S rRNA gene sequences from Yellowstone Park show that this new type of methanotrophic bacteria may be a common inhabitant of extreme environments. This is the first time that a representative of the widely distributed Verrucomicrobia phylum, of which most members remain uncultivated
6
, is coupled to a geochemically relevant reaction.</description><subject>Acids - analysis</subject><subject>Acids - chemistry</subject><subject>Ambient temperature</subject><subject>Atmosphere</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - enzymology</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Clusters</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Emissions</subject><subject>Environmental conditions</subject><subject>Fumaroles</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetics</subject><subject>Geologic Sediments - microbiology</subject><subject>Geology</subject><subject>High temperature</subject><subject>Hot Springs - microbiology</subject><subject>Hot Temperature</subject><subject>Humanities and Social Sciences</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>letter</subject><subject>Membranes</subject><subject>Methane</subject><subject>Methane - chemistry</subject><subject>Methane - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mud</subject><subject>multidisciplinary</subject><subject>Oxidation</subject><subject>Oxygen - analysis</subject><subject>Oxygenases - genetics</subject><subject>Phylogeny</subject><subject>Planctomycetes</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Verrucomicrobia</subject><subject>Volcanic Eruptions</subject><subject>Volcanoes</subject><issn>0028-0836</issn><issn>1476-4687</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN089v0zAUB_AIgVgZnLijCIlfgo7n37Y4VRWwSQMkGHCMHPely5QmmZ1o9MaVf5O_BFet1BZ165RDpOTj77Ps95LkMYEjAky_rW3XewRJKb2TDAhXcsilVneTAQDVQ9BMHiQPQrgAAEEUv58cEA3AGYhB8u4Tdue2bjrftOfzNMequUrb47-__5A0n6c2rfEq_YHe966Zlc43eWnT0KIrMTxM7hW2Cvho9T5Mvn94fzY-Hp5--XgyHp0OnWaUDjGnygphNCWSGEe1E8XEgpbSKWa1kFoagZpbniMXk5yjygtwRY4iByItO0xeLHNb31z2GLpsVgaHVWVrbPqQKc4YaGA0yuc3SmmAx_MxeyGTXGvKyV5ICRGg6f7SFIRQEliEL2-ERAnGtTGwKP70P3rR9L6OZx3juIgKRETDJZraCrOyLuJdWjfFGr2tmhqLMn4eEcO4UlSr23ptlJHcwHoTW9615WW2GXo92kg62oHiM8HYWju3ersFGxVebS2IpsNf3dT2IWQn375uh--1G7mvr7ejs5_jz9vJKx3HJQSPRdb6cmb9PCOQLUY22xjZqJ-sLrnPZzhZ29WMRvBsBWxwtiq8rV0Z1s5oqTUsevrN0oX4q56iX3fLrrr_AO0fT30</recordid><startdate>20071206</startdate><enddate>20071206</enddate><creator>Pol, Arjan</creator><creator>Heijmans, Klaas</creator><creator>Harhangi, Harry R.</creator><creator>Tedesco, Dario</creator><creator>Jetten, Mike S. 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M. ; Op den Camp, Huub J. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c8322-eb27a559821619c28c5fda0866c73a8568695e84a4be45db4e7bf0cfbe5b016a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acids - analysis</topic><topic>Acids - chemistry</topic><topic>Ambient temperature</topic><topic>Atmosphere</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - enzymology</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Clusters</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Emissions</topic><topic>Environmental conditions</topic><topic>Fumaroles</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetics</topic><topic>Geologic Sediments - microbiology</topic><topic>Geology</topic><topic>High temperature</topic><topic>Hot Springs - microbiology</topic><topic>Hot Temperature</topic><topic>Humanities and Social Sciences</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>letter</topic><topic>Membranes</topic><topic>Methane</topic><topic>Methane - chemistry</topic><topic>Methane - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mud</topic><topic>multidisciplinary</topic><topic>Oxidation</topic><topic>Oxygen - analysis</topic><topic>Oxygenases - genetics</topic><topic>Phylogeny</topic><topic>Planctomycetes</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Verrucomicrobia</topic><topic>Volcanic Eruptions</topic><topic>Volcanoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pol, Arjan</creatorcontrib><creatorcontrib>Heijmans, Klaas</creatorcontrib><creatorcontrib>Harhangi, Harry R.</creatorcontrib><creatorcontrib>Tedesco, Dario</creatorcontrib><creatorcontrib>Jetten, Mike S. 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Academic</collection><jtitle>Nature</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pol, Arjan</au><au>Heijmans, Klaas</au><au>Harhangi, Harry R.</au><au>Tedesco, Dario</au><au>Jetten, Mike S. M.</au><au>Op den Camp, Huub J. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methanotrophy below pH 1 by a new Verrucomicrobia species</atitle><jtitle>Nature</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2007-12-06</date><risdate>2007</risdate><volume>450</volume><issue>7171</issue><spage>874</spage><epage>878</epage><pages>874-878</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><eissn>1476-4679</eissn><coden>NATUAS</coden><abstract>Passing the acid test
Bacteria that consume the greenhouse gas methane are potentially important players in the atmospheric budget, with the potential to sop up methane from the Earth's crust that would otherwise contribute to the atmospheric budget. Two new methane-utilizing bacteria have been isolated independently and both break new ground: unlike previous methanotrophic isolates, which are proteobacteria, they belong to the widely distributed Verrucomicrobia phylum. And both isolates display optimum growth and methane oxidation in remarkably acidic conditions, at pHs as low as 0.8 to 2.5.
Acidimethylosilex fumarolicum
SolV was isolated from a fuming vent on the Solfatara volcano near Naples, Italy, and
Methylokorus infernorum
from hot soil in the Hell's Gate (Tikitere) geothermal area of New Zealand.
The isolation of the acidophilic bacterium
Acidimethylosilex fumarolicum
SolV from a fumarole is described. Unlike all previous methanotrophic isolates, which belong to the Alpha- or Gammaproteobacteria, it belongs to the widely distributed Verrumicrobia.
Mud volcanoes, mudpots and fumaroles are remarkable geological features characterized by the emission of gas, water and/or semi-liquid mud matrices
1
with significant methane fluxes to the atmosphere (10
-1
to 10
3
t y
-1
)
2
,
3
,
4
. Environmental conditions in these areas vary from ambient temperature and neutral pH to high temperatures and low pH. Although there are strong indications for biological methane consumption in mud volcanoes
4
,
5
, no methanotrophic bacteria are known that would thrive in the hostile conditions of fumaroles (temperatures up to 70 °C and pH down to 1.8)
2
. The first step in aerobic methane oxidation is performed by a soluble or membrane-bound methane mono-oxygenase. Here we report that
pmoA
(encoding the β-subunit of membrane-bound methane mono-oxygenase) clone libraries, made by using DNA extracted from the Solfatara volcano mudpot and surrounding bare soil near the fumaroles, showed clusters of novel and distant
pmoA
genes. After methanotrophic enrichment at 50 °C and pH 2.0 the most distant cluster, sharing less than 50% identity with any other described
pmoA
gene, was represented in the culture. Finally we isolated an acidiphilic methanotrophic bacterium
Acidimethylosilex fumarolicum
SolV belonging to the Planctomycetes/Verrucomicrobia/Chlamydiae superphylum
6
, ‘outside’ the subphyla of the Alpha- and Gammaproteobacteria containing the established methanotrophs. This bacterium grows under oxygen limitation on methane as the sole source of energy, down to pH 0.8—far below the pH optimum of any previously described methanotroph.
A. fumarolicum
SolV has three different
pmoA
genes, with two that are very similar to sequences retrieved from the mudpot. Highly homologous environmental 16S rRNA gene sequences from Yellowstone Park show that this new type of methanotrophic bacteria may be a common inhabitant of extreme environments. This is the first time that a representative of the widely distributed Verrucomicrobia phylum, of which most members remain uncultivated
6
, is coupled to a geochemically relevant reaction.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>18004305</pmid><doi>10.1038/nature06222</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature, 2007-12, Vol.450 (7171), p.874-878 |
| issn | 0028-0836 1476-4687 1476-4679 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743308032 |
| source | MEDLINE; SpringerLink Journals; Nature Journals Online |
| subjects | Acids - analysis Acids - chemistry Ambient temperature Atmosphere Bacteria Bacteria - classification Bacteria - enzymology Bacteria - genetics Bacteria - metabolism Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacteriology Biological and medical sciences Clusters Deoxyribonucleic acid DNA Emissions Environmental conditions Fumaroles Fundamental and applied biological sciences. Psychology Gene Library Genes Genes, Bacterial - genetics Genetics Geologic Sediments - microbiology Geology High temperature Hot Springs - microbiology Hot Temperature Humanities and Social Sciences Hydrogen-Ion Concentration In Situ Hybridization, Fluorescence letter Membranes Methane Methane - chemistry Methane - metabolism Microbiology Molecular Sequence Data Mud multidisciplinary Oxidation Oxygen - analysis Oxygenases - genetics Phylogeny Planctomycetes RNA, Ribosomal, 16S - genetics Science Science (multidisciplinary) Verrucomicrobia Volcanic Eruptions Volcanoes |
| title | Methanotrophy below pH 1 by a new Verrucomicrobia species |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T05%3A08%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Methanotrophy%20below%20pH%E2%80%891%20by%20a%20new%20Verrucomicrobia%20species&rft.jtitle=Nature&rft.au=Pol,%20Arjan&rft.date=2007-12-06&rft.volume=450&rft.issue=7171&rft.spage=874&rft.epage=878&rft.pages=874-878&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/nature06222&rft_dat=%3Cgale_proqu%3EA193477287%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204548905&rft_id=info:pmid/18004305&rft_galeid=A193477287&rfr_iscdi=true |