Immunization of hu-PBL–SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries
ANTIBODIES are usually prepared from recently boosted animals and reflect ongoing immune responses 1–6 . In humans, this is restrictive as ethical constraints generally prevent antigen-boosting. Therefore the rich memory compartment of human antibody responses remains largely untapped 7 . Severe com...
Gespeichert in:
| Veröffentlicht in: | Nature (London) 1992-01, Vol.355 (6357), p.258-262 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 262 |
|---|---|
| container_issue | 6357 |
| container_start_page | 258 |
| container_title | Nature (London) |
| container_volume | 355 |
| creator | Duchosal, Michel A. Eming, Sabine A. Fischer, Peter Leturcq, Didier Barbas, Carlos F. McConahey, Patricia J. Caothien, Roger H. Thornton, George B. Dixon, Frank J. Burton, Dennis R. |
| description | ANTIBODIES are usually prepared from recently boosted animals and reflect ongoing immune responses
1–6
. In humans, this is restrictive as ethical constraints generally prevent antigen-boosting. Therefore the rich memory compartment of human antibody responses remains largely untapped
7
. Severe combined immune deficiency (SCID) mice
8
populated with human cells
9–11
allow the stimulation of human antibody memory without the usual constraints. Here we show how peripheral blood lymphocytes can be stimulated by antigen to produce large secondary responses after transfer to SCID mice. Specific monoclonal human Fab fragments can then be isolated from the mice by repertoire cloning even when the human donor's last contact with antigen was more than 17 years ago. |
| doi_str_mv | 10.1038/355258a0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_743292795</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1729124</sourcerecordid><originalsourceid>FETCH-LOGICAL-c497t-d6c884f235a90e691168488c1ff14e8fb32a2dcbf7e0248e7b40d59bb43c901c3</originalsourceid><addsrcrecordid>eNqF0c9qFTEUBvAglnqtgi8gBBGti9H8T2apV1svXFBQ10OSydybMknaZGahK9_BN-yTmDJXCwq6yuL7cc4hHwCPMHqJEVWvKOeEK43ugBVmUjRMKHkXrBAiqkGKinvgfikXCCGOJTsGx1hSTAhZgbIJYY7-m558ijANcD83H99sr7__-LTevIXBWwd17OG0dzC7Yme3oKAjDCkmO6aoR3imDRyy3gUXp1JxTvNuD20Kxkc9peyrGb3JOntXHoCjQY_FPTy8J-DL2bvP6_fN9sP5Zv1621jWyqnphVWKDYRy3SInWoyFYkpZPAyYOTUYSjTprRmkQ4QpJw1DPW-NYdS2CFt6Ap4vcy9zuppdmbrgi3XjqKNLc-kko6QlsuVVPvu3JFLyuv6_EAtBuWhv4JM_4EWac_2q0hHEGMWUoYpOF2RzKiW7obvMPuj8tcOou-m1-9VrpY8P82YTXH8LlyJr_vSQ62L1WLuI1pffjHNBiKSVvVhYqUncuXx71l8rfwIO2LbG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>204431340</pqid></control><display><type>article</type><title>Immunization of hu-PBL–SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Nature Journals Online</source><creator>Duchosal, Michel A. ; Eming, Sabine A. ; Fischer, Peter ; Leturcq, Didier ; Barbas, Carlos F. ; McConahey, Patricia J. ; Caothien, Roger H. ; Thornton, George B. ; Dixon, Frank J. ; Burton, Dennis R.</creator><creatorcontrib>Duchosal, Michel A. ; Eming, Sabine A. ; Fischer, Peter ; Leturcq, Didier ; Barbas, Carlos F. ; McConahey, Patricia J. ; Caothien, Roger H. ; Thornton, George B. ; Dixon, Frank J. ; Burton, Dennis R.</creatorcontrib><description>ANTIBODIES are usually prepared from recently boosted animals and reflect ongoing immune responses
1–6
. In humans, this is restrictive as ethical constraints generally prevent antigen-boosting. Therefore the rich memory compartment of human antibody responses remains largely untapped
7
. Severe combined immune deficiency (SCID) mice
8
populated with human cells
9–11
allow the stimulation of human antibody memory without the usual constraints. Here we show how peripheral blood lymphocytes can be stimulated by antigen to produce large secondary responses after transfer to SCID mice. Specific monoclonal human Fab fragments can then be isolated from the mice by repertoire cloning even when the human donor's last contact with antigen was more than 17 years ago.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/355258a0</identifier><identifier>PMID: 1731222</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - immunology ; Base Sequence ; Biological and medical sciences ; Blood ; Cloning ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Library ; Hepatitis B Core Antigens - immunology ; Humanities and Social Sciences ; Humans ; Immunization ; Immunoglobulin Fab Fragments - immunology ; Immunoglobulin G - immunology ; Immunoglobulin Heavy Chains - immunology ; Immunoglobulin Light Chains ; Immunologic Memory ; letter ; Lymphocyte Transfusion ; Lymphocytes ; Lymphocytes - immunology ; Medical research ; Mice ; Mice, SCID ; Molecular immunology ; Molecular Sequence Data ; multidisciplinary ; Oligodeoxyribonucleotides ; Rodents ; Science ; Science (multidisciplinary) ; Techniques ; Transplantation, Heterologous</subject><ispartof>Nature (London), 1992-01, Vol.355 (6357), p.258-262</ispartof><rights>Springer Nature Limited 1992</rights><rights>1992 INIST-CNRS</rights><rights>Copyright Macmillan Journals Ltd. Jan 16, 1992</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-d6c884f235a90e691168488c1ff14e8fb32a2dcbf7e0248e7b40d59bb43c901c3</citedby><cites>FETCH-LOGICAL-c497t-d6c884f235a90e691168488c1ff14e8fb32a2dcbf7e0248e7b40d59bb43c901c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/355258a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/355258a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5562273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1731222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duchosal, Michel A.</creatorcontrib><creatorcontrib>Eming, Sabine A.</creatorcontrib><creatorcontrib>Fischer, Peter</creatorcontrib><creatorcontrib>Leturcq, Didier</creatorcontrib><creatorcontrib>Barbas, Carlos F.</creatorcontrib><creatorcontrib>McConahey, Patricia J.</creatorcontrib><creatorcontrib>Caothien, Roger H.</creatorcontrib><creatorcontrib>Thornton, George B.</creatorcontrib><creatorcontrib>Dixon, Frank J.</creatorcontrib><creatorcontrib>Burton, Dennis R.</creatorcontrib><title>Immunization of hu-PBL–SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>ANTIBODIES are usually prepared from recently boosted animals and reflect ongoing immune responses
1–6
. In humans, this is restrictive as ethical constraints generally prevent antigen-boosting. Therefore the rich memory compartment of human antibody responses remains largely untapped
7
. Severe combined immune deficiency (SCID) mice
8
populated with human cells
9–11
allow the stimulation of human antibody memory without the usual constraints. Here we show how peripheral blood lymphocytes can be stimulated by antigen to produce large secondary responses after transfer to SCID mice. Specific monoclonal human Fab fragments can then be isolated from the mice by repertoire cloning even when the human donor's last contact with antigen was more than 17 years ago.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Cloning</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Library</subject><subject>Hepatitis B Core Antigens - immunology</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immunization</subject><subject>Immunoglobulin Fab Fragments - immunology</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin Heavy Chains - immunology</subject><subject>Immunoglobulin Light Chains</subject><subject>Immunologic Memory</subject><subject>letter</subject><subject>Lymphocyte Transfusion</subject><subject>Lymphocytes</subject><subject>Lymphocytes - immunology</subject><subject>Medical research</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>multidisciplinary</subject><subject>Oligodeoxyribonucleotides</subject><subject>Rodents</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Techniques</subject><subject>Transplantation, Heterologous</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0c9qFTEUBvAglnqtgi8gBBGti9H8T2apV1svXFBQ10OSydybMknaZGahK9_BN-yTmDJXCwq6yuL7cc4hHwCPMHqJEVWvKOeEK43ugBVmUjRMKHkXrBAiqkGKinvgfikXCCGOJTsGx1hSTAhZgbIJYY7-m558ijANcD83H99sr7__-LTevIXBWwd17OG0dzC7Yme3oKAjDCkmO6aoR3imDRyy3gUXp1JxTvNuD20Kxkc9peyrGb3JOntXHoCjQY_FPTy8J-DL2bvP6_fN9sP5Zv1621jWyqnphVWKDYRy3SInWoyFYkpZPAyYOTUYSjTprRmkQ4QpJw1DPW-NYdS2CFt6Ap4vcy9zuppdmbrgi3XjqKNLc-kko6QlsuVVPvu3JFLyuv6_EAtBuWhv4JM_4EWac_2q0hHEGMWUoYpOF2RzKiW7obvMPuj8tcOou-m1-9VrpY8P82YTXH8LlyJr_vSQ62L1WLuI1pffjHNBiKSVvVhYqUncuXx71l8rfwIO2LbG</recordid><startdate>19920116</startdate><enddate>19920116</enddate><creator>Duchosal, Michel A.</creator><creator>Eming, Sabine A.</creator><creator>Fischer, Peter</creator><creator>Leturcq, Didier</creator><creator>Barbas, Carlos F.</creator><creator>McConahey, Patricia J.</creator><creator>Caothien, Roger H.</creator><creator>Thornton, George B.</creator><creator>Dixon, Frank J.</creator><creator>Burton, Dennis R.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7TG</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>S0X</scope><scope>SOI</scope><scope>7QO</scope><scope>7X8</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>19920116</creationdate><title>Immunization of hu-PBL–SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries</title><author>Duchosal, Michel A. ; Eming, Sabine A. ; Fischer, Peter ; Leturcq, Didier ; Barbas, Carlos F. ; McConahey, Patricia J. ; Caothien, Roger H. ; Thornton, George B. ; Dixon, Frank J. ; Burton, Dennis R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-d6c884f235a90e691168488c1ff14e8fb32a2dcbf7e0248e7b40d59bb43c901c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Cloning</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Library</topic><topic>Hepatitis B Core Antigens - immunology</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Immunization</topic><topic>Immunoglobulin Fab Fragments - immunology</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulin Heavy Chains - immunology</topic><topic>Immunoglobulin Light Chains</topic><topic>Immunologic Memory</topic><topic>letter</topic><topic>Lymphocyte Transfusion</topic><topic>Lymphocytes</topic><topic>Lymphocytes - immunology</topic><topic>Medical research</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>multidisciplinary</topic><topic>Oligodeoxyribonucleotides</topic><topic>Rodents</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Techniques</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duchosal, Michel A.</creatorcontrib><creatorcontrib>Eming, Sabine A.</creatorcontrib><creatorcontrib>Fischer, Peter</creatorcontrib><creatorcontrib>Leturcq, Didier</creatorcontrib><creatorcontrib>Barbas, Carlos F.</creatorcontrib><creatorcontrib>McConahey, Patricia J.</creatorcontrib><creatorcontrib>Caothien, Roger H.</creatorcontrib><creatorcontrib>Thornton, George B.</creatorcontrib><creatorcontrib>Dixon, Frank J.</creatorcontrib><creatorcontrib>Burton, Dennis R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>Environment Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duchosal, Michel A.</au><au>Eming, Sabine A.</au><au>Fischer, Peter</au><au>Leturcq, Didier</au><au>Barbas, Carlos F.</au><au>McConahey, Patricia J.</au><au>Caothien, Roger H.</au><au>Thornton, George B.</au><au>Dixon, Frank J.</au><au>Burton, Dennis R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunization of hu-PBL–SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1992-01-16</date><risdate>1992</risdate><volume>355</volume><issue>6357</issue><spage>258</spage><epage>262</epage><pages>258-262</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>ANTIBODIES are usually prepared from recently boosted animals and reflect ongoing immune responses
1–6
. In humans, this is restrictive as ethical constraints generally prevent antigen-boosting. Therefore the rich memory compartment of human antibody responses remains largely untapped
7
. Severe combined immune deficiency (SCID) mice
8
populated with human cells
9–11
allow the stimulation of human antibody memory without the usual constraints. Here we show how peripheral blood lymphocytes can be stimulated by antigen to produce large secondary responses after transfer to SCID mice. Specific monoclonal human Fab fragments can then be isolated from the mice by repertoire cloning even when the human donor's last contact with antigen was more than 17 years ago.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>1731222</pmid><doi>10.1038/355258a0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1992-01, Vol.355 (6357), p.258-262 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743292795 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Base Sequence Biological and medical sciences Blood Cloning Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Library Hepatitis B Core Antigens - immunology Humanities and Social Sciences Humans Immunization Immunoglobulin Fab Fragments - immunology Immunoglobulin G - immunology Immunoglobulin Heavy Chains - immunology Immunoglobulin Light Chains Immunologic Memory letter Lymphocyte Transfusion Lymphocytes Lymphocytes - immunology Medical research Mice Mice, SCID Molecular immunology Molecular Sequence Data multidisciplinary Oligodeoxyribonucleotides Rodents Science Science (multidisciplinary) Techniques Transplantation, Heterologous |
| title | Immunization of hu-PBL–SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T00%3A38%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunization%20of%20hu-PBL%E2%80%93SCID%20mice%20and%20the%20rescue%20of%20human%20monoclonal%20Fab%20fragments%20through%20combinatorial%20libraries&rft.jtitle=Nature%20(London)&rft.au=Duchosal,%20Michel%20A.&rft.date=1992-01-16&rft.volume=355&rft.issue=6357&rft.spage=258&rft.epage=262&rft.pages=258-262&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/355258a0&rft_dat=%3Cproquest_cross%3E1729124%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204431340&rft_id=info:pmid/1731222&rfr_iscdi=true |