Molecular cloning of the phytase gene from Citrobacter braakii and its expression in Saccharomyces cerevisiae

The gene, appA, encoding phytase was cloned from a size-selected genomic library of Citrobacter braakii YH-15 by Southern hybridization using a degenerate probe based on the N-terminal amino acid sequence of the phytase. The deduced amino acid sequence of appA contained the N-terminal RHGXRXP motif...

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Veröffentlicht in:	Biotechnology letters 2006, Vol.28 (1), p.33-38
Hauptverfasser:	KIM, Young-Ok, KIM, Han-Woo, LEE, Jung-Ho, KIM, Kyung-Kil, LEE, Sang-Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	6-Phytase - chemistry 6-Phytase - genetics 6-Phytase - metabolism Amino Acid Motifs Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology Chromosomes, Bacterial Citrobacter Citrobacter - genetics Cloning, Molecular Conserved Sequence Electrophoresis, Polyacrylamide Gel Enzyme Stability Escherichia coli Fundamental and applied biological sciences. Psychology Gene Expression Molecular Sequence Data Phylogeny Physical Chromosome Mapping Recombinant Proteins - chemistry Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Salmonella typhimurium Sequence Homology, Amino Acid Temperature
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