Microbial degradation of poly(butylene succinate) by Fusarium solani in soil environments

The use of mulch made of biodegradable plastic in agriculture is expected to help solve the problem of the enormous amount of plastic waste emission, and to save the labor of removing the mulch after harvesting crops. In this study, we isolated a microorganism possessing the ability to degrade one o...

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Veröffentlicht in:Polymer degradation and stability 2010-02, Vol.95 (2), p.138-143
Hauptverfasser: Abe, Michiharu, Kobayashi, Koji, Honma, Naoya, Nakasaki, Kiyohiko
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container_title Polymer degradation and stability
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creator Abe, Michiharu
Kobayashi, Koji
Honma, Naoya
Nakasaki, Kiyohiko
description The use of mulch made of biodegradable plastic in agriculture is expected to help solve the problem of the enormous amount of plastic waste emission, and to save the labor of removing the mulch after harvesting crops. In this study, we isolated a microorganism possessing the ability to degrade one of the promising biodegradable plastics, poly(butylene succinate) (PBS), and investigated the degradation characteristics of the microorganism in soil environments. Fungal strain WF-6, belonging to Fusarium solani, that had not been reported could be isolated from farmland as the PBS-degrading microorganism. Strain WF-6 degraded 2.8 percent of the PBS in a 14-day experimental run in a sterile soil environment, as determined by measuring CO 2 evolution. Furthermore, we ascertained that the degradability of strain WF-6 was enhanced by co-culturing with the newly isolated bacterial strain Stenotrophomonas maltophilia YB-6, which itself does not show PBS-degrading activity. We then investigated the effects of cell density of the indigenous microorganisms in the soil environments on the degradability of the co-culture of strains WF-6 and YB-6 by inoculating these strains into non-sterilized and partially sterilized soils, which contained 10 8, 10 6, and 10 3 CFU/g-dry solid of soil of indigenous microorganisms. The degradability strongly depended on the cell density level of the indigenous microorganisms and was remarkably diminished when the cell concentration level was the highest, 10 8 CFU/g-dry solid. Quantitative PCR analysis revealed that the growth of strains WF-6 and YB-6 was inhibited in the non-sterile soil environment with the highest cell density level of the indigenous microorganisms.
doi_str_mv 10.1016/j.polymdegradstab.2009.11.042
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source Elsevier ScienceDirect Journals Complete
subjects Ageing
Applied sciences
Biodegradable plastic
Cumulative emission of carbon
Exact sciences and technology
Fusarium solani
Microbial degradation
Physical properties
Poly(butylene succinate)
Polymer industry, paints, wood
Properties and testing
Stenotrophomonas maltophilia
Technology of polymers
title Microbial degradation of poly(butylene succinate) by Fusarium solani in soil environments
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