Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being. ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instance...
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| Veröffentlicht in: | Nature 2006-01, Vol.439 (7073), p.216-219 |
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| creator | Lu, Shi-Jiang Chung, Young Johnson, Julie Meisner, Lorraine Becker, Sandy Lanza, Robert Klimanskaya, Irina Marh, Joel |
| description | The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being. ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instances, from cleavage stage embryos. So far, there have been no reports in the literature of stem cell lines derived using an approach that does not require embryo destruction. Here we report an alternative method of establishing ES cell lines-using a technique of single-cell embryo biopsy similar to that used in pre-implantation genetic diagnosis of genetic defects-that does not interfere with the developmental potential of embryos. Five putative ES and seven trophoblast stem (TS) cell lines were produced from single blastomeres, which maintained normal karyotype and markers of pluripotency or TS cells for up to more than 50 passages. The ES cells differentiated into derivatives of all three germ layers in vitro and in teratomas, and showed germ line transmission. Single-blastomere-biopsied embryos developed to term without a reduction in their developmental capacity. The ability to generate human ES cells without the destruction of ex utero embryos would reduce or eliminate the ethical concerns of many. |
| doi_str_mv | 10.1038/nature04277 |
| format | Article |
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ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instances, from cleavage stage embryos. So far, there have been no reports in the literature of stem cell lines derived using an approach that does not require embryo destruction. Here we report an alternative method of establishing ES cell lines-using a technique of single-cell embryo biopsy similar to that used in pre-implantation genetic diagnosis of genetic defects-that does not interfere with the developmental potential of embryos. Five putative ES and seven trophoblast stem (TS) cell lines were produced from single blastomeres, which maintained normal karyotype and markers of pluripotency or TS cells for up to more than 50 passages. The ES cells differentiated into derivatives of all three germ layers in vitro and in teratomas, and showed germ line transmission. Single-blastomere-biopsied embryos developed to term without a reduction in their developmental capacity. The ability to generate human ES cells without the destruction of ex utero embryos would reduce or eliminate the ethical concerns of many.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/nature04277</identifier><identifier>PMID: 16227970</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Animals ; Biological and medical sciences ; Biomedical research ; Biopsy ; Blastomeres - cytology ; Cell Culture Techniques ; Cell Differentiation ; Cell Separation - methods ; Cells, Cultured ; Embryo Research ; Embryology: invertebrates and vertebrates. Teratology ; Embryos ; Fundamental and applied biological sciences. Psychology ; Karyotypes ; Karyotyping ; Mice ; Molecular embryology ; Rodents ; Stem cells ; Stem Cells - cytology ; Teratoma ; Trophoblasts - cytology</subject><ispartof>Nature, 2006-01, Vol.439 (7073), p.216-219</ispartof><rights>2006 INIST-CNRS</rights><rights>COPYRIGHT 2006 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 12, 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c675t-fda9b2ed0f7b7c741382c508893c14b9d2f729aaba4fb19e4b7b26307e9f18d53</citedby><cites>FETCH-LOGICAL-c675t-fda9b2ed0f7b7c741382c508893c14b9d2f729aaba4fb19e4b7b26307e9f18d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,2728,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17398089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16227970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Shi-Jiang</creatorcontrib><creatorcontrib>Chung, Young</creatorcontrib><creatorcontrib>Johnson, Julie</creatorcontrib><creatorcontrib>Meisner, Lorraine</creatorcontrib><creatorcontrib>Becker, Sandy</creatorcontrib><creatorcontrib>Lanza, Robert</creatorcontrib><creatorcontrib>Klimanskaya, Irina</creatorcontrib><creatorcontrib>Marh, Joel</creatorcontrib><title>Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres</title><title>Nature</title><addtitle>Nature</addtitle><description>The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being. ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instances, from cleavage stage embryos. So far, there have been no reports in the literature of stem cell lines derived using an approach that does not require embryo destruction. Here we report an alternative method of establishing ES cell lines-using a technique of single-cell embryo biopsy similar to that used in pre-implantation genetic diagnosis of genetic defects-that does not interfere with the developmental potential of embryos. Five putative ES and seven trophoblast stem (TS) cell lines were produced from single blastomeres, which maintained normal karyotype and markers of pluripotency or TS cells for up to more than 50 passages. The ES cells differentiated into derivatives of all three germ layers in vitro and in teratomas, and showed germ line transmission. Single-blastomere-biopsied embryos developed to term without a reduction in their developmental capacity. 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Psychology</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>Mice</subject><subject>Molecular embryology</subject><subject>Rodents</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Teratoma</subject><subject>Trophoblasts - cytology</subject><issn>0028-0836</issn><issn>1476-4687</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0t1rFDEQAPAgir2ePvkuq6AisjVfu8k-HkfVQlGoFR9Dkp0cKbvZa7Ir9r9vyh3tnZxKHgLhl8nMZBB6QfAJwUx-DHqcImBOhXiEZoSLuuS1FI_RDGMqSyxZfYSOU7rCGFdE8KfoiNSUikbgGbo47U28GYK3hQ5tAb_HqOH-KI3QFxa6ruh8gFS0EP0vaAsXh75IPqw6KPphSlCYTqdx6CFCeoaeON0leL7d5-jHp9PL5Zfy_Nvns-XivLS1qMbStboxFFrshBFWcMIktRWWsmGWcNO01AnaaG00d4Y0wI0wtGZYQOOIbCs2R-82cddxuJ4gjar36S5ZHSDnpARnuSWE8Szf_lNSiZmQTP4XEoGbqsp6jl7_Aa-GKYZcrqKYV1zwXM8clRu00h0oH9yQm2tXECDqbgjgfD5eEFnxusa7Qfe8XftrtYtODqC8Wui9PRj1_d6FbMb8zSs9paTOvl_s2w9_t4vLn8uvB7WNQ0oRnFpH3-t4owhWd6OpdkYz65fblk2mh_bBbmcxgzdboJPVnYs6WJ8enGCNxHk65ujVxm2i34Pdx24Bje30cg</recordid><startdate>20060112</startdate><enddate>20060112</enddate><creator>Lu, Shi-Jiang</creator><creator>Chung, Young</creator><creator>Johnson, Julie</creator><creator>Meisner, Lorraine</creator><creator>Becker, Sandy</creator><creator>Lanza, Robert</creator><creator>Klimanskaya, Irina</creator><creator>Marh, Joel</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ATWCN</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7TG</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>S0X</scope><scope>SOI</scope><scope>7QO</scope><scope>7U5</scope><scope>L7M</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>20060112</creationdate><title>Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres</title><author>Lu, Shi-Jiang ; Chung, Young ; Johnson, Julie ; Meisner, Lorraine ; Becker, Sandy ; Lanza, Robert ; Klimanskaya, Irina ; Marh, Joel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c675t-fda9b2ed0f7b7c741382c508893c14b9d2f729aaba4fb19e4b7b26307e9f18d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical research</topic><topic>Biopsy</topic><topic>Blastomeres - cytology</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cell Separation - methods</topic><topic>Cells, Cultured</topic><topic>Embryo Research</topic><topic>Embryology: invertebrates and vertebrates. 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| subjects | Animals Biological and medical sciences Biomedical research Biopsy Blastomeres - cytology Cell Culture Techniques Cell Differentiation Cell Separation - methods Cells, Cultured Embryo Research Embryology: invertebrates and vertebrates. Teratology Embryos Fundamental and applied biological sciences. Psychology Karyotypes Karyotyping Mice Molecular embryology Rodents Stem cells Stem Cells - cytology Teratoma Trophoblasts - cytology |
| title | Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres |
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