Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of...
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| Veröffentlicht in: | Nature (London) 2000-11, Vol.408 (6810), p.331-336 |
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| creator | Gönczy, Pierre Echeverri, Christophe Oegema, Karen Coulson, Alan Jones, Steven J. M. Copley, Richard R. Duperon, John Oegema, Jeff Brehm, Michael Cassin, Etienne Hannak, Eva Kirkham, Matthew Pichler, Silke Flohrs, Kathrin Goessen, Anoesjka Leidel, Sebastian Alleaume, Anne-Marie Martin, Cécilie Özlü, Nurhan Bork, Peer Hyman, Anthony A. |
| description | Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in
Caenorhabditis elegans
. We inhibited the expression of ∼96% of the ∼2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an
in vivo
time-lapse differential interference contrast microscopy assay, we identified 133 genes (∼6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in
C. elegans
embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that ∼47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well. |
| doi_str_mv | 10.1038/35042526 |
| format | Article |
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Caenorhabditis elegans
. We inhibited the expression of ∼96% of the ∼2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an
in vivo
time-lapse differential interference contrast microscopy assay, we identified 133 genes (∼6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in
C. elegans
embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that ∼47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/35042526</identifier><identifier>PMID: 11099034</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biological and medical sciences ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - physiology ; Cell cycle, cell proliferation ; Cell division ; Cell Division - genetics ; Cell physiology ; chromosome III ; Chromosomes ; Embryos ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Helminth ; Genomics ; Humanities and Social Sciences ; Insects ; Molecular and cellular biology ; multidisciplinary ; Open Reading Frames ; Ribonucleic acid ; RNA ; RNA, Helminth ; RNA-mediated interference ; Science ; Science (multidisciplinary) ; Worms</subject><ispartof>Nature (London), 2000-11, Vol.408 (6810), p.331-336</ispartof><rights>Macmillan Magazines Ltd. 2000</rights><rights>2001 INIST-CNRS</rights><rights>COPYRIGHT 2000 Nature Publishing Group</rights><rights>Copyright Macmillan Journals Ltd. Nov 16, 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-d01b91092274a184a324d2b64db2a9f14b0251b06fd93cd201648ea1fa06b9663</citedby><cites>FETCH-LOGICAL-c668t-d01b91092274a184a324d2b64db2a9f14b0251b06fd93cd201648ea1fa06b9663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/35042526$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/35042526$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=835731$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11099034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gönczy, Pierre</creatorcontrib><creatorcontrib>Echeverri, Christophe</creatorcontrib><creatorcontrib>Oegema, Karen</creatorcontrib><creatorcontrib>Coulson, Alan</creatorcontrib><creatorcontrib>Jones, Steven J. M.</creatorcontrib><creatorcontrib>Copley, Richard R.</creatorcontrib><creatorcontrib>Duperon, John</creatorcontrib><creatorcontrib>Oegema, Jeff</creatorcontrib><creatorcontrib>Brehm, Michael</creatorcontrib><creatorcontrib>Cassin, Etienne</creatorcontrib><creatorcontrib>Hannak, Eva</creatorcontrib><creatorcontrib>Kirkham, Matthew</creatorcontrib><creatorcontrib>Pichler, Silke</creatorcontrib><creatorcontrib>Flohrs, Kathrin</creatorcontrib><creatorcontrib>Goessen, Anoesjka</creatorcontrib><creatorcontrib>Leidel, Sebastian</creatorcontrib><creatorcontrib>Alleaume, Anne-Marie</creatorcontrib><creatorcontrib>Martin, Cécilie</creatorcontrib><creatorcontrib>Özlü, Nurhan</creatorcontrib><creatorcontrib>Bork, Peer</creatorcontrib><creatorcontrib>Hyman, Anthony A.</creatorcontrib><title>Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in
Caenorhabditis elegans
. We inhibited the expression of ∼96% of the ∼2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an
in vivo
time-lapse differential interference contrast microscopy assay, we identified 133 genes (∼6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in
C. elegans
embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that ∼47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell division</subject><subject>Cell Division - genetics</subject><subject>Cell physiology</subject><subject>chromosome III</subject><subject>Chromosomes</subject><subject>Embryos</subject><subject>Fundamental and applied biological sciences. 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M. ; Copley, Richard R. ; Duperon, John ; Oegema, Jeff ; Brehm, Michael ; Cassin, Etienne ; Hannak, Eva ; Kirkham, Matthew ; Pichler, Silke ; Flohrs, Kathrin ; Goessen, Anoesjka ; Leidel, Sebastian ; Alleaume, Anne-Marie ; Martin, Cécilie ; Özlü, Nurhan ; Bork, Peer ; Hyman, Anthony A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c668t-d01b91092274a184a324d2b64db2a9f14b0251b06fd93cd201648ea1fa06b9663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans - physiology</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell division</topic><topic>Cell Division - genetics</topic><topic>Cell physiology</topic><topic>chromosome III</topic><topic>Chromosomes</topic><topic>Embryos</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Helminth</topic><topic>Genomics</topic><topic>Humanities and Social Sciences</topic><topic>Insects</topic><topic>Molecular and cellular biology</topic><topic>multidisciplinary</topic><topic>Open Reading Frames</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Helminth</topic><topic>RNA-mediated interference</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gönczy, Pierre</creatorcontrib><creatorcontrib>Echeverri, Christophe</creatorcontrib><creatorcontrib>Oegema, Karen</creatorcontrib><creatorcontrib>Coulson, Alan</creatorcontrib><creatorcontrib>Jones, Steven J. 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M.</au><au>Copley, Richard R.</au><au>Duperon, John</au><au>Oegema, Jeff</au><au>Brehm, Michael</au><au>Cassin, Etienne</au><au>Hannak, Eva</au><au>Kirkham, Matthew</au><au>Pichler, Silke</au><au>Flohrs, Kathrin</au><au>Goessen, Anoesjka</au><au>Leidel, Sebastian</au><au>Alleaume, Anne-Marie</au><au>Martin, Cécilie</au><au>Özlü, Nurhan</au><au>Bork, Peer</au><au>Hyman, Anthony A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2000-11-16</date><risdate>2000</risdate><volume>408</volume><issue>6810</issue><spage>331</spage><epage>336</epage><pages>331-336</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in
Caenorhabditis elegans
. We inhibited the expression of ∼96% of the ∼2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an
in vivo
time-lapse differential interference contrast microscopy assay, we identified 133 genes (∼6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in
C. elegans
embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that ∼47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>11099034</pmid><doi>10.1038/35042526</doi><tpages>6</tpages></addata></record> |
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| recordid | cdi_proquest_miscellaneous_743203572 |
| source | MEDLINE; SpringerLink Journals (MCLS); Nature |
| subjects | Animals Biological and medical sciences Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans - physiology Cell cycle, cell proliferation Cell division Cell Division - genetics Cell physiology chromosome III Chromosomes Embryos Fundamental and applied biological sciences. Psychology Genes Genes, Helminth Genomics Humanities and Social Sciences Insects Molecular and cellular biology multidisciplinary Open Reading Frames Ribonucleic acid RNA RNA, Helminth RNA-mediated interference Science Science (multidisciplinary) Worms |
| title | Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T00%3A54%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20genomic%20analysis%20of%20cell%20division%20in%20C.%20elegans%20using%20RNAi%20of%20genes%20on%20chromosome%20III&rft.jtitle=Nature%20(London)&rft.au=G%C3%B6nczy,%20Pierre&rft.date=2000-11-16&rft.volume=408&rft.issue=6810&rft.spage=331&rft.epage=336&rft.pages=331-336&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/35042526&rft_dat=%3Cgale_proqu%3EA188051527%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204467559&rft_id=info:pmid/11099034&rft_galeid=A188051527&rfr_iscdi=true |