Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells
Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell...
Gespeichert in:
| Veröffentlicht in: | Biotechnology and applied biochemistry 2008-06, Vol.50 (2), p.89-96 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 96 |
|---|---|
| container_issue | 2 |
| container_start_page | 89 |
| container_title | Biotechnology and applied biochemistry |
| container_volume | 50 |
| creator | Chen, Zhengtang Zhuo, Wenlei Wang, Yan Ao, Xujun An, Jianghong |
| description | Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our preliminary research, and was hypothesized to play an essential role in cell motility and tumour lymphatic metastasis. In the present study we found that, as expected, suppression of layilin expression by RNA interference significantly inhibited A549‐cell invasion and migration in vitro and lymphatic metastasis in vivo and thereby resulted in the increased survival of tumour‐bearing mice. These findings suggested that layilin might play crucial roles in lymphatic metastasis of lung carcinoma and that suppression of layilin expression might be a promising strategy for treatment of human lung carcinoma. |
| doi_str_mv | 10.1042/BA20070138 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_743188528</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69203629</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5623-deb61bef53c19c2eb3497653e2573082a0ee93544b842ac05e84010a152c43443</originalsourceid><addsrcrecordid>eNqFkd1uEzEQhVcIREPhhgdAvqFIKFvGf7vey7RAQIqC-FMvV15ntjF4vcHeTclr8MQ4JGrvimRpxtJ3zox9suw5hXMKgr25mDGAEihXD7IJFSXkqhTiYTYBpWQuJOUn2ZMYfwCAKhV7nJ3QVBhXMMn-vO1vfB7wenR6sL0nfUuc3lln_ZRo4vstOrLeaTeG3mtPAhrcDH2Ykq3V5MtyRqwfMLQY0BucptvaNnaIqdnquDfUfkXcrtusk78hHQ46pmPjftJ67JKnG_01mUlREYPOxafZo1a7iM-O9TT7_v7dt8sP-eLT_OPlbJEbWTCer7ApaIOt5IZWhmHDRVUWkiOTJQfFNCBWXArRKMG0AYlKAAVNJTOCC8FPs1cH303of40Yh7qzcb-B9tiPsS4Fp-kDmUrk2b1kUTHgBav-C6acAITYg68PoAl9jAHbehNsp8OuplDvQ63vQk3wi6Pr2HS4ukOPKSbg5RHQ0WjXBu2NjbdcWq4S9B93fuBurMPdPSNTe5GenwT5QWDjgL9vBTr8rIuSl7K-Ws5r-pXNl5-voF7wv_UhxSs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20000449</pqid></control><display><type>article</type><title>Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Chen, Zhengtang ; Zhuo, Wenlei ; Wang, Yan ; Ao, Xujun ; An, Jianghong</creator><creatorcontrib>Chen, Zhengtang ; Zhuo, Wenlei ; Wang, Yan ; Ao, Xujun ; An, Jianghong</creatorcontrib><description>Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our preliminary research, and was hypothesized to play an essential role in cell motility and tumour lymphatic metastasis. In the present study we found that, as expected, suppression of layilin expression by RNA interference significantly inhibited A549‐cell invasion and migration in vitro and lymphatic metastasis in vivo and thereby resulted in the increased survival of tumour‐bearing mice. These findings suggested that layilin might play crucial roles in lymphatic metastasis of lung carcinoma and that suppression of layilin expression might be a promising strategy for treatment of human lung carcinoma.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1042/BA20070138</identifier><identifier>PMID: 17822380</identifier><identifier>CODEN: BABIEC</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>A549 human lung cells ; Animals ; Biological and medical sciences ; Biotechnology ; Carrier Proteins - genetics ; Cell Line, Tumor ; Down-Regulation ; Fundamental and applied biological sciences. Psychology ; Genetic Therapy - methods ; Humans ; Hya ; hyaluronan (HYA) receptor ; Hyaluronan Receptors - genetics ; layilin ; lung carcinoma ; Lung Neoplasms - genetics ; Lung Neoplasms - prevention & control ; Lung Neoplasms - secondary ; lymphatic invasion and metastasis ; Lymphatic Metastasis ; Membrane Glycoproteins - genetics ; Mice ; Mice, Inbred BALB C ; RNA Interference ; RNA interference (RNAi) gene therapy ; Survival Rate</subject><ispartof>Biotechnology and applied biochemistry, 2008-06, Vol.50 (2), p.89-96</ispartof><rights>2008 International Union of Biochemistry and Molecular Biology</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5623-deb61bef53c19c2eb3497653e2573082a0ee93544b842ac05e84010a152c43443</citedby><cites>FETCH-LOGICAL-c5623-deb61bef53c19c2eb3497653e2573082a0ee93544b842ac05e84010a152c43443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FBA20070138$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FBA20070138$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20394180$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17822380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Zhengtang</creatorcontrib><creatorcontrib>Zhuo, Wenlei</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Ao, Xujun</creatorcontrib><creatorcontrib>An, Jianghong</creatorcontrib><title>Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnol Appl Biochem</addtitle><description>Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our preliminary research, and was hypothesized to play an essential role in cell motility and tumour lymphatic metastasis. In the present study we found that, as expected, suppression of layilin expression by RNA interference significantly inhibited A549‐cell invasion and migration in vitro and lymphatic metastasis in vivo and thereby resulted in the increased survival of tumour‐bearing mice. These findings suggested that layilin might play crucial roles in lymphatic metastasis of lung carcinoma and that suppression of layilin expression might be a promising strategy for treatment of human lung carcinoma.</description><subject>A549 human lung cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carrier Proteins - genetics</subject><subject>Cell Line, Tumor</subject><subject>Down-Regulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Therapy - methods</subject><subject>Humans</subject><subject>Hya</subject><subject>hyaluronan (HYA) receptor</subject><subject>Hyaluronan Receptors - genetics</subject><subject>layilin</subject><subject>lung carcinoma</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - prevention & control</subject><subject>Lung Neoplasms - secondary</subject><subject>lymphatic invasion and metastasis</subject><subject>Lymphatic Metastasis</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>RNA Interference</subject><subject>RNA interference (RNAi) gene therapy</subject><subject>Survival Rate</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd1uEzEQhVcIREPhhgdAvqFIKFvGf7vey7RAQIqC-FMvV15ntjF4vcHeTclr8MQ4JGrvimRpxtJ3zox9suw5hXMKgr25mDGAEihXD7IJFSXkqhTiYTYBpWQuJOUn2ZMYfwCAKhV7nJ3QVBhXMMn-vO1vfB7wenR6sL0nfUuc3lln_ZRo4vstOrLeaTeG3mtPAhrcDH2Ykq3V5MtyRqwfMLQY0BucptvaNnaIqdnquDfUfkXcrtusk78hHQ46pmPjftJ67JKnG_01mUlREYPOxafZo1a7iM-O9TT7_v7dt8sP-eLT_OPlbJEbWTCer7ApaIOt5IZWhmHDRVUWkiOTJQfFNCBWXArRKMG0AYlKAAVNJTOCC8FPs1cH303of40Yh7qzcb-B9tiPsS4Fp-kDmUrk2b1kUTHgBav-C6acAITYg68PoAl9jAHbehNsp8OuplDvQ63vQk3wi6Pr2HS4ukOPKSbg5RHQ0WjXBu2NjbdcWq4S9B93fuBurMPdPSNTe5GenwT5QWDjgL9vBTr8rIuSl7K-Ws5r-pXNl5-voF7wv_UhxSs</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Chen, Zhengtang</creator><creator>Zhuo, Wenlei</creator><creator>Wang, Yan</creator><creator>Ao, Xujun</creator><creator>An, Jianghong</creator><general>Blackwell Publishing Ltd</general><general>Portland Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>7TB</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>200806</creationdate><title>Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells</title><author>Chen, Zhengtang ; Zhuo, Wenlei ; Wang, Yan ; Ao, Xujun ; An, Jianghong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5623-deb61bef53c19c2eb3497653e2573082a0ee93544b842ac05e84010a152c43443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>A549 human lung cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carrier Proteins - genetics</topic><topic>Cell Line, Tumor</topic><topic>Down-Regulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Therapy - methods</topic><topic>Humans</topic><topic>Hya</topic><topic>hyaluronan (HYA) receptor</topic><topic>Hyaluronan Receptors - genetics</topic><topic>layilin</topic><topic>lung carcinoma</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - prevention & control</topic><topic>Lung Neoplasms - secondary</topic><topic>lymphatic invasion and metastasis</topic><topic>Lymphatic Metastasis</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>RNA Interference</topic><topic>RNA interference (RNAi) gene therapy</topic><topic>Survival Rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Zhengtang</creatorcontrib><creatorcontrib>Zhuo, Wenlei</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Ao, Xujun</creatorcontrib><creatorcontrib>An, Jianghong</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Zhengtang</au><au>Zhuo, Wenlei</au><au>Wang, Yan</au><au>Ao, Xujun</au><au>An, Jianghong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnol Appl Biochem</addtitle><date>2008-06</date><risdate>2008</risdate><volume>50</volume><issue>2</issue><spage>89</spage><epage>96</epage><pages>89-96</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><coden>BABIEC</coden><abstract>Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our preliminary research, and was hypothesized to play an essential role in cell motility and tumour lymphatic metastasis. In the present study we found that, as expected, suppression of layilin expression by RNA interference significantly inhibited A549‐cell invasion and migration in vitro and lymphatic metastasis in vivo and thereby resulted in the increased survival of tumour‐bearing mice. These findings suggested that layilin might play crucial roles in lymphatic metastasis of lung carcinoma and that suppression of layilin expression might be a promising strategy for treatment of human lung carcinoma.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17822380</pmid><doi>10.1042/BA20070138</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0885-4513 |
| ispartof | Biotechnology and applied biochemistry, 2008-06, Vol.50 (2), p.89-96 |
| issn | 0885-4513 1470-8744 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743188528 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | A549 human lung cells Animals Biological and medical sciences Biotechnology Carrier Proteins - genetics Cell Line, Tumor Down-Regulation Fundamental and applied biological sciences. Psychology Genetic Therapy - methods Humans Hya hyaluronan (HYA) receptor Hyaluronan Receptors - genetics layilin lung carcinoma Lung Neoplasms - genetics Lung Neoplasms - prevention & control Lung Neoplasms - secondary lymphatic invasion and metastasis Lymphatic Metastasis Membrane Glycoproteins - genetics Mice Mice, Inbred BALB C RNA Interference RNA interference (RNAi) gene therapy Survival Rate |
| title | Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T03%3A47%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Down-regulation%20of%20layilin,%20a%20novel%20hyaluronan%20receptor,%20via%20RNA%20interference,%20inhibits%20invasion%20and%20lymphatic%20metastasis%20of%20human%20lung%20A549%20cells&rft.jtitle=Biotechnology%20and%20applied%20biochemistry&rft.au=Chen,%20Zhengtang&rft.date=2008-06&rft.volume=50&rft.issue=2&rft.spage=89&rft.epage=96&rft.pages=89-96&rft.issn=0885-4513&rft.eissn=1470-8744&rft.coden=BABIEC&rft_id=info:doi/10.1042/BA20070138&rft_dat=%3Cproquest_cross%3E69203629%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20000449&rft_id=info:pmid/17822380&rfr_iscdi=true |