Distinct Roles for TBP and TBP-Like Factor in Early Embryonic Gene Transcription in Xenopus
The TATA-binding protein (TBP) is believed to function as a key component of the general transcription machinery. We tested the role of TBP during the onset of embryonic transcription by antisense oligonucleotide-mediated turnover of maternal TBP messenger RNA. Embryos without detectable TBP initiat...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2000-12, Vol.290 (5500), p.2312-2315 |
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| creator | Gert Jan C. Veenstra Weeks, Daniel L. Wolffe, Alan P. |
| description | The TATA-binding protein (TBP) is believed to function as a key component of the general transcription machinery. We tested the role of TBP during the onset of embryonic transcription by antisense oligonucleotide-mediated turnover of maternal TBP messenger RNA. Embryos without detectable TBP initiated gastrulation but died before completing gastrulation. The expression of many genes transcribed by RNA polymerase II and III was reduced; however, some genes were transcribed with an efficiency identical to that of TBP-containing embryos. Using a similar antisense strategy, we found that the TBP-like factor TLF/TRF2 is essential for development past the mid-blastula stage. Because TBP and a TLF factor play complementary roles in embryonic development, our results indicate that although similar mechanistic roles exist in common, TBP and TLF function differentially to control transcription of specific genes. |
| doi_str_mv | 10.1126/science.290.5500.2312 |
| format | Article |
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Veenstra ; Weeks, Daniel L. ; Wolffe, Alan P.</creator><creatorcontrib>Gert Jan C. Veenstra ; Weeks, Daniel L. ; Wolffe, Alan P.</creatorcontrib><description>The TATA-binding protein (TBP) is believed to function as a key component of the general transcription machinery. We tested the role of TBP during the onset of embryonic transcription by antisense oligonucleotide-mediated turnover of maternal TBP messenger RNA. Embryos without detectable TBP initiated gastrulation but died before completing gastrulation. The expression of many genes transcribed by RNA polymerase II and III was reduced; however, some genes were transcribed with an efficiency identical to that of TBP-containing embryos. Using a similar antisense strategy, we found that the TBP-like factor TLF/TRF2 is essential for development past the mid-blastula stage. Because TBP and a TLF factor play complementary roles in embryonic development, our results indicate that although similar mechanistic roles exist in common, TBP and TLF function differentially to control transcription of specific genes.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.290.5500.2312</identifier><identifier>PMID: 11125147</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Analysis ; Animal embryology ; Animals ; Biological and medical sciences ; Blastocyst - metabolism ; Blastula ; Cholinergics ; Complementary DNA ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Embryo, Nonmammalian - metabolism ; Embryology ; Embryonic Development ; Embryos ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gastrula - metabolism ; Gene Expression Regulation, Developmental ; Genes ; Genetic aspects ; HeLa cells ; Memory ; Messenger RNA ; Molecular and cellular biology ; Molecular genetics ; Oligonucleotides ; Oligonucleotides, Antisense - metabolism ; Oligonucleotides, Antisense - pharmacology ; Protein binding ; Proteins ; Reptiles & amphibians ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; TATA-Box Binding Protein ; TBP-like factor ; Telomeric Repeat Binding Protein 2 ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. 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Veenstra</creatorcontrib><creatorcontrib>Weeks, Daniel L.</creatorcontrib><creatorcontrib>Wolffe, Alan P.</creatorcontrib><title>Distinct Roles for TBP and TBP-Like Factor in Early Embryonic Gene Transcription in Xenopus</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The TATA-binding protein (TBP) is believed to function as a key component of the general transcription machinery. We tested the role of TBP during the onset of embryonic transcription by antisense oligonucleotide-mediated turnover of maternal TBP messenger RNA. Embryos without detectable TBP initiated gastrulation but died before completing gastrulation. The expression of many genes transcribed by RNA polymerase II and III was reduced; however, some genes were transcribed with an efficiency identical to that of TBP-containing embryos. Using a similar antisense strategy, we found that the TBP-like factor TLF/TRF2 is essential for development past the mid-blastula stage. Because TBP and a TLF factor play complementary roles in embryonic development, our results indicate that although similar mechanistic roles exist in common, TBP and TLF function differentially to control transcription of specific genes.</description><subject>Analysis</subject><subject>Animal embryology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - metabolism</subject><subject>Blastula</subject><subject>Cholinergics</subject><subject>Complementary DNA</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Embryology</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. 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| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2000-12, Vol.290 (5500), p.2312-2315 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_743183398 |
| source | MEDLINE; Science Magazine; Jstor Complete Legacy |
| subjects | Analysis Animal embryology Animals Biological and medical sciences Blastocyst - metabolism Blastula Cholinergics Complementary DNA DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Embryo, Nonmammalian - metabolism Embryology Embryonic Development Embryos Freshwater Fundamental and applied biological sciences. Psychology Gastrula - metabolism Gene Expression Regulation, Developmental Genes Genetic aspects HeLa cells Memory Messenger RNA Molecular and cellular biology Molecular genetics Oligonucleotides Oligonucleotides, Antisense - metabolism Oligonucleotides, Antisense - pharmacology Protein binding Proteins Reptiles & amphibians Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism TATA-Box Binding Protein TBP-like factor Telomeric Repeat Binding Protein 2 Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transcripts (Written Records) Translation Xenopus Xenopus - embryology |
| title | Distinct Roles for TBP and TBP-Like Factor in Early Embryonic Gene Transcription in Xenopus |
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