Culture of isolated single cells from Taxus suspensions for the propagation of superior cell populations

Single cells isolated from aggregated Taxus cuspidata cultures via enzymatic digestion were grown in suspension culture. High seeding density (4 x 10(5 )cells/ml) and the addition of cell-free conditioned medium were essential for growth. Doubling the concentration of the nutrients [ascorbic acid (1...

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Veröffentlicht in:Biotechnology letters 2005-11, Vol.27 (21), p.1725-1730
Hauptverfasser: NAILL, Michael C, ROBERTS, Susan C
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Sprache:eng
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