Characterization of pbpA and pbp2 encoding penicillin-binding proteins located on the downstream of clavulanic acid gene cluster in Streptomyces clavuligerus

Two genes, pbpA (orf18) and pbp2 (orf19) located on the downstream of clavulanic acid (CA) gene cluster of Streptomyces clavuligerus were cloned into pET-28a(+), and confirmed to encode a family of high molecular-weight penicillin-binding proteins (PBPs). Both genes were amplified from genomic DNA b...

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Veröffentlicht in:	Biotechnology letters 2006-03, Vol.28 (6), p.409-417
Hauptverfasser:	Ishida, Kenji, Hung, Trinh Viet, Liou, Kwangkyoung, Lee, Hei Chan, Shin, Chang-Hun, Sohng, Jae Kyung
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Clavulanic Acid - metabolism E coli Escherichia coli Genes Genetic Vectors - genetics Microbial Sensitivity Tests Molecular Sequence Data Penicillin Penicillin-Binding Proteins - biosynthesis Penicillin-Binding Proteins - chemistry Penicillin-Binding Proteins - genetics Proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Streptomyces - genetics Streptomyces - metabolism Streptomyces clavuligerus
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