Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media

SFM (serum‐free medium) is preferred to media containing animal‐derived components when culturing mammalian cells for the production of therapeutic recombinant proteins and mAbs (monoclonal antibodies). Nonetheless, eliminating animal‐derived components from media can strongly modify culture perform...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2007-06, Vol.47 (2), p.113-124
Hauptverfasser:	Serrato, J. Antonio, Hernández, Vanessa, Estrada-Mondaca, Sandino, Palomares, Laura A., Ramírez, Octavio T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - metabolism Biological and medical sciences Biotechnology Cell Culture Techniques - methods Cells, Cultured chemically defined medium Culture Media - chemistry Culture Media - metabolism Culture Media, Serum-Free exoglycosidase Fundamental and applied biological sciences. Psychology Glycosylation Hybridomas - metabolism metabolism monoclonal antibody Protein Engineering - methods Serum - metabolism serum-free medium
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