Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein

The green-fluorescent protein (GFP) that functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea has been renatured with up to 90% yield following acid, base, or guanidine denaturation. Renaturation, following pH neutralization or simple dilution of guanidine, proceeds with...

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Veröffentlicht in:	Biochemistry (Easton) 1982-09, Vol.21 (19), p.4535-4540
Hauptverfasser:	Ward, William W, Bokman, Stephen H
Format:	Artikel
Sprache:	eng
Schlagworte:	Aequorin - isolation & purification Aequorin - metabolism Animals Circular Dichroism Cnidaria - metabolism Guanidine Guanidines Hydrogen-Ion Concentration Luminescent Proteins - isolation & purification Protein Conformation Protein Denaturation Scyphozoa Scyphozoa - metabolism Spectrometry, Fluorescence Spectrophotometry
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