Plasma ADP levels: Direct determination with luciferase luminescence using a biometer
A method is described for the determination of low plasma levels of adenosine-5′-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following...
Gespeichert in:
| Veröffentlicht in: | Clinical biochemistry 1978-01, Vol.11 (5), p.190-193 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 193 |
|---|---|
| container_issue | 5 |
| container_start_page | 190 |
| container_title | Clinical biochemistry |
| container_volume | 11 |
| creator | Jabs, Clarence M. Ferrell, William J. Robb, Herbert J. |
| description | A method is described for the determination of low plasma levels of adenosine-5′-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 μmol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 ± 0.025 (10) μmol/liter and rabbit plasma concentration were 0.07 ± 0.05 (5) μmol/liter. Several other possible applications of the method are discussed. |
| doi_str_mv | 10.1016/S0009-9120(78)80027-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74301805</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009912078800277</els_id><sourcerecordid>74301805</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-12f5cf68631ac4ae7dbd9e12cbd01a5fe75d1284a2d9e4d2a3d0b4d4cc0318e43</originalsourceid><addsrcrecordid>eNqFkEtPxCAUhYnxNY7-A01YGV1UgdJC3ZjJ-EwmcRKdNaFwq5g-FFqN_17mEbeugHvOuYd8CJ1QckEJzS-fCSFFUlBGzoQ8l4QwkYgtNKJSpAkr0nQbjf4s--gghPf4ZFzme2hXsILmZIQW81qHRuPJzRzX8AV1uMI3zoPpsYUefONa3buuxd-uf8P1YFwFXgeI1yhBMNAawENw7SvWuHRds0wdop1K1wGONucYLe5uX6YPyezp_nE6mSWGk7xPKKsyU-UyT6k2XIOwpS2AMlNaQnVWgcgsZZJrFsfcMp1aUnLLjSEplcDTMTpd7_3w3ecAoVeNi1-qa91CNwQleEqoJFk0Zmuj8V0IHir14V2j_Y-iRC1pqhVNtUSlhFQrmkrE3PGmYCgbsH-pNb4oX6_lyA2-HHgVjFsSsSuEynbun4JfbByFIA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74301805</pqid></control><display><type>article</type><title>Plasma ADP levels: Direct determination with luciferase luminescence using a biometer</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Jabs, Clarence M. ; Ferrell, William J. ; Robb, Herbert J.</creator><creatorcontrib>Jabs, Clarence M. ; Ferrell, William J. ; Robb, Herbert J.</creatorcontrib><description>A method is described for the determination of low plasma levels of adenosine-5′-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 μmol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 ± 0.025 (10) μmol/liter and rabbit plasma concentration were 0.07 ± 0.05 (5) μmol/liter. Several other possible applications of the method are discussed.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/S0009-9120(78)80027-7</identifier><identifier>PMID: 729160</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Diphosphate - blood ; Animals ; Creatine Kinase ; Drug Stability ; Firefly Luciferin ; Humans ; Luciferases ; Luminescent Measurements ; Methods ; Rabbits ; Shock, Septic - blood ; Species Specificity</subject><ispartof>Clinical biochemistry, 1978-01, Vol.11 (5), p.190-193</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-12f5cf68631ac4ae7dbd9e12cbd01a5fe75d1284a2d9e4d2a3d0b4d4cc0318e43</citedby><cites>FETCH-LOGICAL-c406t-12f5cf68631ac4ae7dbd9e12cbd01a5fe75d1284a2d9e4d2a3d0b4d4cc0318e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-9120(78)80027-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/729160$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jabs, Clarence M.</creatorcontrib><creatorcontrib>Ferrell, William J.</creatorcontrib><creatorcontrib>Robb, Herbert J.</creatorcontrib><title>Plasma ADP levels: Direct determination with luciferase luminescence using a biometer</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>A method is described for the determination of low plasma levels of adenosine-5′-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 μmol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 ± 0.025 (10) μmol/liter and rabbit plasma concentration were 0.07 ± 0.05 (5) μmol/liter. Several other possible applications of the method are discussed.</description><subject>Adenosine Diphosphate - blood</subject><subject>Animals</subject><subject>Creatine Kinase</subject><subject>Drug Stability</subject><subject>Firefly Luciferin</subject><subject>Humans</subject><subject>Luciferases</subject><subject>Luminescent Measurements</subject><subject>Methods</subject><subject>Rabbits</subject><subject>Shock, Septic - blood</subject><subject>Species Specificity</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPxCAUhYnxNY7-A01YGV1UgdJC3ZjJ-EwmcRKdNaFwq5g-FFqN_17mEbeugHvOuYd8CJ1QckEJzS-fCSFFUlBGzoQ8l4QwkYgtNKJSpAkr0nQbjf4s--gghPf4ZFzme2hXsILmZIQW81qHRuPJzRzX8AV1uMI3zoPpsYUefONa3buuxd-uf8P1YFwFXgeI1yhBMNAawENw7SvWuHRds0wdop1K1wGONucYLe5uX6YPyezp_nE6mSWGk7xPKKsyU-UyT6k2XIOwpS2AMlNaQnVWgcgsZZJrFsfcMp1aUnLLjSEplcDTMTpd7_3w3ecAoVeNi1-qa91CNwQleEqoJFk0Zmuj8V0IHir14V2j_Y-iRC1pqhVNtUSlhFQrmkrE3PGmYCgbsH-pNb4oX6_lyA2-HHgVjFsSsSuEynbun4JfbByFIA</recordid><startdate>19780101</startdate><enddate>19780101</enddate><creator>Jabs, Clarence M.</creator><creator>Ferrell, William J.</creator><creator>Robb, Herbert J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780101</creationdate><title>Plasma ADP levels: Direct determination with luciferase luminescence using a biometer</title><author>Jabs, Clarence M. ; Ferrell, William J. ; Robb, Herbert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-12f5cf68631ac4ae7dbd9e12cbd01a5fe75d1284a2d9e4d2a3d0b4d4cc0318e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Adenosine Diphosphate - blood</topic><topic>Animals</topic><topic>Creatine Kinase</topic><topic>Drug Stability</topic><topic>Firefly Luciferin</topic><topic>Humans</topic><topic>Luciferases</topic><topic>Luminescent Measurements</topic><topic>Methods</topic><topic>Rabbits</topic><topic>Shock, Septic - blood</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jabs, Clarence M.</creatorcontrib><creatorcontrib>Ferrell, William J.</creatorcontrib><creatorcontrib>Robb, Herbert J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jabs, Clarence M.</au><au>Ferrell, William J.</au><au>Robb, Herbert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma ADP levels: Direct determination with luciferase luminescence using a biometer</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>1978-01-01</date><risdate>1978</risdate><volume>11</volume><issue>5</issue><spage>190</spage><epage>193</epage><pages>190-193</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>A method is described for the determination of low plasma levels of adenosine-5′-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 μmol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 ± 0.025 (10) μmol/liter and rabbit plasma concentration were 0.07 ± 0.05 (5) μmol/liter. Several other possible applications of the method are discussed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>729160</pmid><doi>10.1016/S0009-9120(78)80027-7</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9120 |
| ispartof | Clinical biochemistry, 1978-01, Vol.11 (5), p.190-193 |
| issn | 0009-9120 1873-2933 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74301805 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adenosine Diphosphate - blood Animals Creatine Kinase Drug Stability Firefly Luciferin Humans Luciferases Luminescent Measurements Methods Rabbits Shock, Septic - blood Species Specificity |
| title | Plasma ADP levels: Direct determination with luciferase luminescence using a biometer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T08%3A45%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20ADP%20levels:%20Direct%20determination%20with%20luciferase%20luminescence%20using%20a%20biometer&rft.jtitle=Clinical%20biochemistry&rft.au=Jabs,%20Clarence%20M.&rft.date=1978-01-01&rft.volume=11&rft.issue=5&rft.spage=190&rft.epage=193&rft.pages=190-193&rft.issn=0009-9120&rft.eissn=1873-2933&rft_id=info:doi/10.1016/S0009-9120(78)80027-7&rft_dat=%3Cproquest_cross%3E74301805%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74301805&rft_id=info:pmid/729160&rft_els_id=S0009912078800277&rfr_iscdi=true |