Effect of dibutyryl cyclic AMP on leucine incorporation in L5178Y cells II. Effect of actinomycin and heat shock
3H‐leucine incorporation was assayed in L5178Y cells as a measure for protein synthesis. Protein synthesis was inhibited by short duration heat shock, actinomycin, cordycepin or a combination of these agents. Dibutyryl cyclic AMP had little or no effect upon protein synthesis in control cells. Dibut...
Gespeichert in:
| Veröffentlicht in: | Journal of cellular physiology 1978-12, Vol.97 (3), p.329-333 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 333 |
|---|---|
| container_issue | 3 |
| container_start_page | 329 |
| container_title | Journal of cellular physiology |
| container_volume | 97 |
| creator | Fuhr, Joseph E. |
| description | 3H‐leucine incorporation was assayed in L5178Y cells as a measure for protein synthesis. Protein synthesis was inhibited by short duration heat shock, actinomycin, cordycepin or a combination of these agents. Dibutyryl cyclic AMP had little or no effect upon protein synthesis in control cells. Dibutyryl cyclic AMP, however, stimulated protein synthesis in cells previously heat shocked and/or inhibited by actinomycin or cordycepin. The results have been interpreted to indicate that the cyclic nucleotide stimulated protein synthesis by influencing the metabolism of some species of RNA which may regulate translation. |
| doi_str_mv | 10.1002/jcp.1040970307 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74295220</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>74295220</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2917-c7dcda7e1e26091e5d15a84cbc52dac47a283074c4de470158c0694eefbfa2333</originalsourceid><addsrcrecordid>eNqFkL1vFDEQxS1EBEegpaFxRbeHP8_rMjqF46IDUgARNJZvdlZxsrde7F3B_vc4XJSIimpGM-_9NPMIec3ZkjMm3t3AUBrFrGGSmSdkwUtbqZUWT8miCHhlteLPyYucbxhj1kr5jJzUUgq5IMN52yKMNLa0CftpnNPcUZihC0DPPl7S2NMOJwg90tBDTENMfgxlGnq609zU3ylg12W63S7pI8vDGPp4mIuR-r6h1-hHmq8j3L4kJ63vMr66r6fk6_vzL-sP1e7zZrs-21UgLDcVmAYab5CjWDHLUTdc-1rBHrRoPCjjRV2-VaAaVIZxXQNbWYXY7lsvpJSn5O2RO6T4c8I8ukPId6f6HuOUnVHCaiFYES6PQkgx54StG1I4-DQ7ztxdwK4E7B4DLoY39-Rpf8DmQf430bK1x-2v0OH8H5a7WF_-Q66O3pBH_P3g9enWrYw02l192rhvmx9SXezW7kr-AWoQlZg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74295220</pqid></control><display><type>article</type><title>Effect of dibutyryl cyclic AMP on leucine incorporation in L5178Y cells II. Effect of actinomycin and heat shock</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Fuhr, Joseph E.</creator><creatorcontrib>Fuhr, Joseph E.</creatorcontrib><description>3H‐leucine incorporation was assayed in L5178Y cells as a measure for protein synthesis. Protein synthesis was inhibited by short duration heat shock, actinomycin, cordycepin or a combination of these agents. Dibutyryl cyclic AMP had little or no effect upon protein synthesis in control cells. Dibutyryl cyclic AMP, however, stimulated protein synthesis in cells previously heat shocked and/or inhibited by actinomycin or cordycepin. The results have been interpreted to indicate that the cyclic nucleotide stimulated protein synthesis by influencing the metabolism of some species of RNA which may regulate translation.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.1040970307</identifier><identifier>PMID: 83323</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Bucladesine - pharmacology ; Cell Line ; Dactinomycin - pharmacology ; Deoxyadenosines - pharmacology ; Hot Temperature ; Leucine - metabolism ; Protein Biosynthesis ; RNA - metabolism ; Time Factors ; Tritium</subject><ispartof>Journal of cellular physiology, 1978-12, Vol.97 (3), p.329-333</ispartof><rights>Copyright © 1978 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2917-c7dcda7e1e26091e5d15a84cbc52dac47a283074c4de470158c0694eefbfa2333</citedby><cites>FETCH-LOGICAL-c2917-c7dcda7e1e26091e5d15a84cbc52dac47a283074c4de470158c0694eefbfa2333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.1040970307$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.1040970307$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/83323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fuhr, Joseph E.</creatorcontrib><title>Effect of dibutyryl cyclic AMP on leucine incorporation in L5178Y cells II. Effect of actinomycin and heat shock</title><title>Journal of cellular physiology</title><addtitle>J. Cell. Physiol</addtitle><description>3H‐leucine incorporation was assayed in L5178Y cells as a measure for protein synthesis. Protein synthesis was inhibited by short duration heat shock, actinomycin, cordycepin or a combination of these agents. Dibutyryl cyclic AMP had little or no effect upon protein synthesis in control cells. Dibutyryl cyclic AMP, however, stimulated protein synthesis in cells previously heat shocked and/or inhibited by actinomycin or cordycepin. The results have been interpreted to indicate that the cyclic nucleotide stimulated protein synthesis by influencing the metabolism of some species of RNA which may regulate translation.</description><subject>Bucladesine - pharmacology</subject><subject>Cell Line</subject><subject>Dactinomycin - pharmacology</subject><subject>Deoxyadenosines - pharmacology</subject><subject>Hot Temperature</subject><subject>Leucine - metabolism</subject><subject>Protein Biosynthesis</subject><subject>RNA - metabolism</subject><subject>Time Factors</subject><subject>Tritium</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1vFDEQxS1EBEegpaFxRbeHP8_rMjqF46IDUgARNJZvdlZxsrde7F3B_vc4XJSIimpGM-_9NPMIec3ZkjMm3t3AUBrFrGGSmSdkwUtbqZUWT8miCHhlteLPyYucbxhj1kr5jJzUUgq5IMN52yKMNLa0CftpnNPcUZihC0DPPl7S2NMOJwg90tBDTENMfgxlGnq609zU3ylg12W63S7pI8vDGPp4mIuR-r6h1-hHmq8j3L4kJ63vMr66r6fk6_vzL-sP1e7zZrs-21UgLDcVmAYab5CjWDHLUTdc-1rBHrRoPCjjRV2-VaAaVIZxXQNbWYXY7lsvpJSn5O2RO6T4c8I8ukPId6f6HuOUnVHCaiFYES6PQkgx54StG1I4-DQ7ztxdwK4E7B4DLoY39-Rpf8DmQf430bK1x-2v0OH8H5a7WF_-Q66O3pBH_P3g9enWrYw02l192rhvmx9SXezW7kr-AWoQlZg</recordid><startdate>197812</startdate><enddate>197812</enddate><creator>Fuhr, Joseph E.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197812</creationdate><title>Effect of dibutyryl cyclic AMP on leucine incorporation in L5178Y cells II. Effect of actinomycin and heat shock</title><author>Fuhr, Joseph E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2917-c7dcda7e1e26091e5d15a84cbc52dac47a283074c4de470158c0694eefbfa2333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Bucladesine - pharmacology</topic><topic>Cell Line</topic><topic>Dactinomycin - pharmacology</topic><topic>Deoxyadenosines - pharmacology</topic><topic>Hot Temperature</topic><topic>Leucine - metabolism</topic><topic>Protein Biosynthesis</topic><topic>RNA - metabolism</topic><topic>Time Factors</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuhr, Joseph E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuhr, Joseph E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of dibutyryl cyclic AMP on leucine incorporation in L5178Y cells II. Effect of actinomycin and heat shock</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J. Cell. Physiol</addtitle><date>1978-12</date><risdate>1978</risdate><volume>97</volume><issue>3</issue><spage>329</spage><epage>333</epage><pages>329-333</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>3H‐leucine incorporation was assayed in L5178Y cells as a measure for protein synthesis. Protein synthesis was inhibited by short duration heat shock, actinomycin, cordycepin or a combination of these agents. Dibutyryl cyclic AMP had little or no effect upon protein synthesis in control cells. Dibutyryl cyclic AMP, however, stimulated protein synthesis in cells previously heat shocked and/or inhibited by actinomycin or cordycepin. The results have been interpreted to indicate that the cyclic nucleotide stimulated protein synthesis by influencing the metabolism of some species of RNA which may regulate translation.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>83323</pmid><doi>10.1002/jcp.1040970307</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9541 |
| ispartof | Journal of cellular physiology, 1978-12, Vol.97 (3), p.329-333 |
| issn | 0021-9541 1097-4652 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74295220 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Bucladesine - pharmacology Cell Line Dactinomycin - pharmacology Deoxyadenosines - pharmacology Hot Temperature Leucine - metabolism Protein Biosynthesis RNA - metabolism Time Factors Tritium |
| title | Effect of dibutyryl cyclic AMP on leucine incorporation in L5178Y cells II. Effect of actinomycin and heat shock |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T15%3A28%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20dibutyryl%20cyclic%20AMP%20on%20leucine%20incorporation%20in%20L5178Y%20cells%20II.%20Effect%20of%20actinomycin%20and%20heat%20shock&rft.jtitle=Journal%20of%20cellular%20physiology&rft.au=Fuhr,%20Joseph%20E.&rft.date=1978-12&rft.volume=97&rft.issue=3&rft.spage=329&rft.epage=333&rft.pages=329-333&rft.issn=0021-9541&rft.eissn=1097-4652&rft_id=info:doi/10.1002/jcp.1040970307&rft_dat=%3Cproquest_cross%3E74295220%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74295220&rft_id=info:pmid/83323&rfr_iscdi=true |