A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II
Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate struct...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1982-12, Vol.257 (23), p.14365-14369 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14369 |
|---|---|
| container_issue | 23 |
| container_start_page | 14365 |
| container_title | The Journal of biological chemistry |
| container_volume | 257 |
| creator | Magnani, J L Nilsson, B Brockhaus, M Zopf, D Steplewski, Z Koprowski, H Ginsburg, V |
| description | Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate structure is probably (formula: see text). This oligosaccharide is a sialyl derivative of lacto-N-fucopentaose II, a hapten of the human Lea blood group antigen. About 30 micrograms of ganglioside is obtained from 1 g of cells, wet weight. The ganglioside was detected by autoradiography in lipid extracts of some carcinomas and of meconium, but not in lipid extracts of normal adult tissues. Antigen was detected by solid phase radioimmunoassay in lipid extracts from 12 out of 21 adenocarcinomas of the colon, from 4 out of 5 gastric adenocarcinomas, and from 4 out of 7 pancreatic carcinomas. Antigen was not detected in lipid extracts from 5 esophageal carcinomas or from normal colon and gastric mucosa, pancreas, kidney, liver, and bone marrow. |
| doi_str_mv | 10.1016/S0021-9258(19)45389-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74288949</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925819453891</els_id><sourcerecordid>74288949</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-87a04ba7d162be5f8a6a2d6c852646b765ad0fa29955d74086a479dbce012af53</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS0EKtPCI1TKAiG6CNiO7cQrVFX8jFTBApDYWTe2k7kosYfYQzXvwEPj-VG3eGPpnu9cX59LyDWjbxll6t03SjmrNZfdG6ZvhGw6XbMnZMVo19SNZD-fktUj8pxcpvSLliM0uyAXLROcM7Eif2-rOYZopxhgqiBk7KPb184PGLw7FkYfKkgpWoRcSg-YN9UIKS8RQ_Yp48FpIVi_VJgqKGIYJ4wJna9sDBkwYBirhDDtp2OPCWyO9Zd62Nm49YWIyVfr9QvybIAp-Zfn-4r8-Pjh-93n-v7rp_Xd7X1tRaNy3bVARQ-tY4r3Xg4dKOBO2U5yJVTfKgmODsC1ltK1gnYKRKtdbz1lHAbZXJHXp77bJf7elS-YGZP10wTBx10yreBdp4UuoDyBdokpLX4w2wVnWPaGUXPYgjluwRwiNkyb4xYMK77r8wO7fvbu0XWOveivTvoGx80DLt70GO3Gz4bL1vDGFEYd5nx_wnwJ4w_6xSSLvgTtisVm4yL-Z5B_yKKmBw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74288949</pqid></control><display><type>article</type><title>A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Magnani, J L ; Nilsson, B ; Brockhaus, M ; Zopf, D ; Steplewski, Z ; Koprowski, H ; Ginsburg, V</creator><creatorcontrib>Magnani, J L ; Nilsson, B ; Brockhaus, M ; Zopf, D ; Steplewski, Z ; Koprowski, H ; Ginsburg, V</creatorcontrib><description>Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate structure is probably (formula: see text). This oligosaccharide is a sialyl derivative of lacto-N-fucopentaose II, a hapten of the human Lea blood group antigen. About 30 micrograms of ganglioside is obtained from 1 g of cells, wet weight. The ganglioside was detected by autoradiography in lipid extracts of some carcinomas and of meconium, but not in lipid extracts of normal adult tissues. Antigen was detected by solid phase radioimmunoassay in lipid extracts from 12 out of 21 adenocarcinomas of the colon, from 4 out of 5 gastric adenocarcinomas, and from 4 out of 7 pancreatic carcinomas. Antigen was not detected in lipid extracts from 5 esophageal carcinomas or from normal colon and gastric mucosa, pancreas, kidney, liver, and bone marrow.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)45389-1</identifier><identifier>PMID: 7142214</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antibodies, Monoclonal ; Antigens, Neoplasm - analysis ; Cell Line ; Chromatography, Thin Layer ; Colonic Neoplasms - immunology ; Gangliosides - analysis ; Gas Chromatography-Mass Spectrometry ; Humans ; Hybridomas - immunology ; Mice ; Oligosaccharides - analysis ; Radioimmunoassay</subject><ispartof>The Journal of biological chemistry, 1982-12, Vol.257 (23), p.14365-14369</ispartof><rights>1982 © 1982 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-87a04ba7d162be5f8a6a2d6c852646b765ad0fa29955d74086a479dbce012af53</citedby><cites>FETCH-LOGICAL-c436t-87a04ba7d162be5f8a6a2d6c852646b765ad0fa29955d74086a479dbce012af53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7142214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Magnani, J L</creatorcontrib><creatorcontrib>Nilsson, B</creatorcontrib><creatorcontrib>Brockhaus, M</creatorcontrib><creatorcontrib>Zopf, D</creatorcontrib><creatorcontrib>Steplewski, Z</creatorcontrib><creatorcontrib>Koprowski, H</creatorcontrib><creatorcontrib>Ginsburg, V</creatorcontrib><title>A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate structure is probably (formula: see text). This oligosaccharide is a sialyl derivative of lacto-N-fucopentaose II, a hapten of the human Lea blood group antigen. About 30 micrograms of ganglioside is obtained from 1 g of cells, wet weight. The ganglioside was detected by autoradiography in lipid extracts of some carcinomas and of meconium, but not in lipid extracts of normal adult tissues. Antigen was detected by solid phase radioimmunoassay in lipid extracts from 12 out of 21 adenocarcinomas of the colon, from 4 out of 5 gastric adenocarcinomas, and from 4 out of 7 pancreatic carcinomas. Antigen was not detected in lipid extracts from 5 esophageal carcinomas or from normal colon and gastric mucosa, pancreas, kidney, liver, and bone marrow.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Cell Line</subject><subject>Chromatography, Thin Layer</subject><subject>Colonic Neoplasms - immunology</subject><subject>Gangliosides - analysis</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>Hybridomas - immunology</subject><subject>Mice</subject><subject>Oligosaccharides - analysis</subject><subject>Radioimmunoassay</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKtPCI1TKAiG6CNiO7cQrVFX8jFTBApDYWTe2k7kosYfYQzXvwEPj-VG3eGPpnu9cX59LyDWjbxll6t03SjmrNZfdG6ZvhGw6XbMnZMVo19SNZD-fktUj8pxcpvSLliM0uyAXLROcM7Eif2-rOYZopxhgqiBk7KPb184PGLw7FkYfKkgpWoRcSg-YN9UIKS8RQ_Yp48FpIVi_VJgqKGIYJ4wJna9sDBkwYBirhDDtp2OPCWyO9Zd62Nm49YWIyVfr9QvybIAp-Zfn-4r8-Pjh-93n-v7rp_Xd7X1tRaNy3bVARQ-tY4r3Xg4dKOBO2U5yJVTfKgmODsC1ltK1gnYKRKtdbz1lHAbZXJHXp77bJf7elS-YGZP10wTBx10yreBdp4UuoDyBdokpLX4w2wVnWPaGUXPYgjluwRwiNkyb4xYMK77r8wO7fvbu0XWOveivTvoGx80DLt70GO3Gz4bL1vDGFEYd5nx_wnwJ4w_6xSSLvgTtisVm4yL-Z5B_yKKmBw</recordid><startdate>19821210</startdate><enddate>19821210</enddate><creator>Magnani, J L</creator><creator>Nilsson, B</creator><creator>Brockhaus, M</creator><creator>Zopf, D</creator><creator>Steplewski, Z</creator><creator>Koprowski, H</creator><creator>Ginsburg, V</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19821210</creationdate><title>A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II</title><author>Magnani, J L ; Nilsson, B ; Brockhaus, M ; Zopf, D ; Steplewski, Z ; Koprowski, H ; Ginsburg, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-87a04ba7d162be5f8a6a2d6c852646b765ad0fa29955d74086a479dbce012af53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Cell Line</topic><topic>Chromatography, Thin Layer</topic><topic>Colonic Neoplasms - immunology</topic><topic>Gangliosides - analysis</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>Hybridomas - immunology</topic><topic>Mice</topic><topic>Oligosaccharides - analysis</topic><topic>Radioimmunoassay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Magnani, J L</creatorcontrib><creatorcontrib>Nilsson, B</creatorcontrib><creatorcontrib>Brockhaus, M</creatorcontrib><creatorcontrib>Zopf, D</creatorcontrib><creatorcontrib>Steplewski, Z</creatorcontrib><creatorcontrib>Koprowski, H</creatorcontrib><creatorcontrib>Ginsburg, V</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Magnani, J L</au><au>Nilsson, B</au><au>Brockhaus, M</au><au>Zopf, D</au><au>Steplewski, Z</au><au>Koprowski, H</au><au>Ginsburg, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1982-12-10</date><risdate>1982</risdate><volume>257</volume><issue>23</issue><spage>14365</spage><epage>14369</epage><pages>14365-14369</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate structure is probably (formula: see text). This oligosaccharide is a sialyl derivative of lacto-N-fucopentaose II, a hapten of the human Lea blood group antigen. About 30 micrograms of ganglioside is obtained from 1 g of cells, wet weight. The ganglioside was detected by autoradiography in lipid extracts of some carcinomas and of meconium, but not in lipid extracts of normal adult tissues. Antigen was detected by solid phase radioimmunoassay in lipid extracts from 12 out of 21 adenocarcinomas of the colon, from 4 out of 5 gastric adenocarcinomas, and from 4 out of 7 pancreatic carcinomas. Antigen was not detected in lipid extracts from 5 esophageal carcinomas or from normal colon and gastric mucosa, pancreas, kidney, liver, and bone marrow.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7142214</pmid><doi>10.1016/S0021-9258(19)45389-1</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1982-12, Vol.257 (23), p.14365-14369 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74288949 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal Antigens, Neoplasm - analysis Cell Line Chromatography, Thin Layer Colonic Neoplasms - immunology Gangliosides - analysis Gas Chromatography-Mass Spectrometry Humans Hybridomas - immunology Mice Oligosaccharides - analysis Radioimmunoassay |
| title | A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T14%3A40%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20monoclonal%20antibody-defined%20antigen%20associated%20with%20gastrointestinal%20cancer%20is%20a%20ganglioside%20containing%20sialylated%20lacto-N-fucopentaose%20II&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Magnani,%20J%20L&rft.date=1982-12-10&rft.volume=257&rft.issue=23&rft.spage=14365&rft.epage=14369&rft.pages=14365-14369&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1016/S0021-9258(19)45389-1&rft_dat=%3Cproquest_cross%3E74288949%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74288949&rft_id=info:pmid/7142214&rft_els_id=S0021925819453891&rfr_iscdi=true |