Expression of functionality of alpha-chymotrypsin. Effects of guanidine hydrochloride and urea in the onset of denaturation

Crystals of alpha-chymotrypsin (CHT) at equilibrium in solutions of 2.0 M guanidine hydrochloride and 3.0 M urea at pH 3.6 were prepared, three-dimensional X-ray intensities were measured, and difference electron-density maps were calculated and examined. The guanidine hydrochloride derivative displ...

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Veröffentlicht in:	Biochemistry (Easton) 1978-12, Vol.17 (25), p.5460-5468
Hauptverfasser:	Hibbard, L S, Tulinsky, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Chymotrypsin - metabolism Guanidines Protein Binding Protein Conformation Protein Denaturation Urea X-Ray Diffraction
Online-Zugang:	Volltext
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