[13] Purification of PGH-PGE isomerase from sheep vesicular glands

This chapter presents a procedure for the purification of prostaglandin (PG) H–PGE isomerase from sheep vesicular glands. In the purification procedure described in the chapter, deep-frozen sheep seminal vesicles, trimmed free from fat and connective tissue, were homogenized in 75-g portions with 11...

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Veröffentlicht in:	Methods in Enzymology 1982, Vol.86, p.84-91
Hauptverfasser:	Moonen, P., Buytenhek, M., Nugteren, D.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Agarose - methods Chromatography, DEAE-Cellulose - methods Exocrine Glands - enzymology Hydrogen-Ion Concentration Intramolecular Oxidoreductases Isomerases - isolation & purification Microsomes - enzymology Molecular Weight Prostaglandin-E Synthases Sheep
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