The Colorimetric Analysis of Sialic Acid in Human Saliva and Bovine Salivary Mucin
Three colorimetric methods were compared for their suitability for the analysis of sialic acid in human mixed saliva and bovine submaxillary gland mucin. The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of...
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| Veröffentlicht in: | Journal of dental research 1978-11, Vol.57 (11-12), p.995-1002 |
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| container_end_page | 1002 |
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| container_issue | 11-12 |
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| container_title | Journal of dental research |
| container_volume | 57 |
| creator | Pearce, E.I.F. Major, G.N. |
| description | Three colorimetric methods were compared for their suitability for the analysis of sialic acid in human mixed saliva and bovine submaxillary gland mucin. The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of choice for these samples. However, it is not possible to use this method for the analysis of bovine mucins. With these samples the resorcinol method was most suitable, being subject to the least interference and relatively precise. The direct Ehrlich method was least precise overall and gave high values with human whole saliva. |
| doi_str_mv | 10.1177/00220345780570111701 |
| format | Article |
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The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of choice for these samples. However, it is not possible to use this method for the analysis of bovine mucins. With these samples the resorcinol method was most suitable, being subject to the least interference and relatively precise. The direct Ehrlich method was least precise overall and gave high values with human whole saliva.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/00220345780570111701</identifier><identifier>PMID: 281346</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Cattle ; Colorimetry - methods ; Dentistry ; Fucose - analysis ; Glycoproteins - analysis ; Humans ; Hydrolysis ; Mucins - analysis ; Resorcinols ; Saliva - analysis ; Salivary Proteins and Peptides - analysis ; Sialic Acids - analysis ; Thiobarbiturates</subject><ispartof>Journal of dental research, 1978-11, Vol.57 (11-12), p.995-1002</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-989ef8610c57bbe01b07fe84996aab7532dcd62319891d82f45a3d25fc05451e3</citedby><cites>FETCH-LOGICAL-c343t-989ef8610c57bbe01b07fe84996aab7532dcd62319891d82f45a3d25fc05451e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/00220345780570111701$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/00220345780570111701$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/281346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pearce, E.I.F.</creatorcontrib><creatorcontrib>Major, G.N.</creatorcontrib><title>The Colorimetric Analysis of Sialic Acid in Human Saliva and Bovine Salivary Mucin</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Three colorimetric methods were compared for their suitability for the analysis of sialic acid in human mixed saliva and bovine submaxillary gland mucin. The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of choice for these samples. However, it is not possible to use this method for the analysis of bovine mucins. With these samples the resorcinol method was most suitable, being subject to the least interference and relatively precise. The direct Ehrlich method was least precise overall and gave high values with human whole saliva.</description><subject>Animals</subject><subject>Cattle</subject><subject>Colorimetry - methods</subject><subject>Dentistry</subject><subject>Fucose - analysis</subject><subject>Glycoproteins - analysis</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Mucins - analysis</subject><subject>Resorcinols</subject><subject>Saliva - analysis</subject><subject>Salivary Proteins and Peptides - analysis</subject><subject>Sialic Acids - analysis</subject><subject>Thiobarbiturates</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC3EqxT-QQ8-cQt4_YiTY6mAIhUh0XKOHMcBV0lc4qRS_z2OUnFCnFaa_Wa0OwjNgNwBSHlPCKWEcSETIiSBoBE4QRMQnEdEpHCKJgMSDcwluvJ-SwikNGEX6JwmwHg8Qe-bL4MXrnKtrU3XWo3njaoO3nrsSry2qhokbQtsG7zsa9XgddD2CqumwA9ubxtzVNoDfu21ba7RWakqb26Oc4o-nh43i2W0ent-WcxXkWacdVGapKZMYiBayDw3BHIiS5PwNI2VyqVgtNBFTBkEEIqEllwoVlBRaiK4AMOm6HbM3bXuuze-y2rrtakq1RjX-0xyGrOU8gDyEdSt8741ZbYL34Z7MyDZ0GT2V5PBNjvm93ltil_TWF1Yw7j26tNkW9e3oTj_f-QPqbB6Cw</recordid><startdate>197811</startdate><enddate>197811</enddate><creator>Pearce, E.I.F.</creator><creator>Major, G.N.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197811</creationdate><title>The Colorimetric Analysis of Sialic Acid in Human Saliva and Bovine Salivary Mucin</title><author>Pearce, E.I.F. ; Major, G.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-989ef8610c57bbe01b07fe84996aab7532dcd62319891d82f45a3d25fc05451e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Colorimetry - methods</topic><topic>Dentistry</topic><topic>Fucose - analysis</topic><topic>Glycoproteins - analysis</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Mucins - analysis</topic><topic>Resorcinols</topic><topic>Saliva - analysis</topic><topic>Salivary Proteins and Peptides - analysis</topic><topic>Sialic Acids - analysis</topic><topic>Thiobarbiturates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pearce, E.I.F.</creatorcontrib><creatorcontrib>Major, G.N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pearce, E.I.F.</au><au>Major, G.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Colorimetric Analysis of Sialic Acid in Human Saliva and Bovine Salivary Mucin</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>1978-11</date><risdate>1978</risdate><volume>57</volume><issue>11-12</issue><spage>995</spage><epage>1002</epage><pages>995-1002</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><abstract>Three colorimetric methods were compared for their suitability for the analysis of sialic acid in human mixed saliva and bovine submaxillary gland mucin. The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of choice for these samples. However, it is not possible to use this method for the analysis of bovine mucins. With these samples the resorcinol method was most suitable, being subject to the least interference and relatively precise. The direct Ehrlich method was least precise overall and gave high values with human whole saliva.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>281346</pmid><doi>10.1177/00220345780570111701</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of dental research, 1978-11, Vol.57 (11-12), p.995-1002 |
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| language | eng |
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| source | MEDLINE; SAGE Complete |
| subjects | Animals Cattle Colorimetry - methods Dentistry Fucose - analysis Glycoproteins - analysis Humans Hydrolysis Mucins - analysis Resorcinols Saliva - analysis Salivary Proteins and Peptides - analysis Sialic Acids - analysis Thiobarbiturates |
| title | The Colorimetric Analysis of Sialic Acid in Human Saliva and Bovine Salivary Mucin |
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