Polyglutaraldehyde: A new reagent for coupling proteins to microspheres and for labeling cell-surface receptors

Polyglutaraldehyde: A new reagent for coupling proteins to microspheres and for labeling cell-surface receptors

Glutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hydrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was...

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Veröffentlicht in:Journal of immunological methods 1978-01, Vol.24 (3), p.239-250
Hauptverfasser: Rembaum, A., Margel, S., Levy, J.
Format: Artikel
Sprache:eng
Schlagworte:
Aldehydes
Binding Sites, Antibody
Glutaral
Hemoglobins
Immunoglobulin Fc Fragments
Indicators and Reagents
Lymphocytes - immunology
Microspheres
Molecular Weight
Polymers
Receptors, Antigen, B-Cell - metabolism
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container_title Journal of immunological methods
container_volume 24
creator Rembaum, A.
Margel, S.
Levy, J.
description Glutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hydrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was investigated spectrophotometrically as a function of pH and temperature. The reaction of polyglutaraldehyde was found to be faster than that of the monomer. The findings led to successful labeling of human lymphocyte subpopulations.
doi_str_mv 10.1016/0022-1759(78)90128-X
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subjects Aldehydes
Binding Sites, Antibody
Glutaral
Hemoglobins
Immunoglobulin Fc Fragments
Indicators and Reagents
Lymphocytes - immunology
Microspheres
Molecular Weight
Polymers
Receptors, Antigen, B-Cell - metabolism
title Polyglutaraldehyde: A new reagent for coupling proteins to microspheres and for labeling cell-surface receptors
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