Partial purification of centrioles from spleen cells

Isolation of a fraction enriched with centrioles from bovine spleen cells is described. The method involves preparation of a nuclear-centriolar complex and detachment of centrioles from the complex by mechanical treatment in a homogenizer. Purification of centrioles was achieved by centrifugation th...

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Veröffentlicht in:Cell biology international reports 1978-11, Vol.2 (6), p.601-606
Hauptverfasser: Nadezhdina, E.S., Fais, D., Chentsov, Yu.S.
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Sprache:eng
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