Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme
The potentiometric acid-base titration curve of fully protonated lysozyme at ionic strengths of 0.10 and 1.0 m has been performed. The stoichiometry and the p K a values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with p...
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| Veröffentlicht in: | Anal. Biochem.; (United States) 1982-07, Vol.123 (2), p.244-248 |
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| creator | Godinho, O.E.S. Aleixo, L.M. Hora Alves, J.P. |
| description | The potentiometric acid-base titration curve of fully protonated lysozyme at ionic strengths of 0.10 and 1.0
m has been performed. The stoichiometry and the p
K
a values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with p
K
a values of 3.76 and 5.02, the imidazole group with p
K
a 7.37 and the amine group with p
K
a 9.63, have been identified at an ionic strength of 0.10
m at 25.0°C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base. |
| doi_str_mv | 10.1016/0003-2697(82)90441-9 |
| format | Article |
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m has been performed. The stoichiometry and the p
K
a values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with p
K
a values of 3.76 and 5.02, the imidazole group with p
K
a 7.37 and the amine group with p
K
a 9.63, have been identified at an ionic strength of 0.10
m at 25.0°C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(82)90441-9</identifier><identifier>PMID: 7125199</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>400102 - Chemical & Spectral Procedures ; AMINO ACIDS ; AZOLES ; CARBOXYLIC ACIDS ; Chemical Phenomena ; CHEMICAL REACTION KINETICS ; Chemistry ; ENZYMES ; GLYCOSYL HYDROLASES ; HETEROCYCLIC COMPOUNDS ; Hydrogen-Ion Concentration ; HYDROLASES ; IMIDAZOLES ; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ; KINETICS ; LYSOZYME ; MOLECULAR STRUCTURE ; Muramidase - analysis ; O-GLYCOSYL HYDROLASES ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; Osmolar Concentration ; Potentiometry ; REACTION KINETICS ; STOICHIOMETRY ; TITRATION</subject><ispartof>Anal. Biochem.; (United States), 1982-07, Vol.123 (2), p.244-248</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c299t-9b655d0710ce3f665a5c2a2e61742d2a76997c72d5979e35161c8aa52b7f48c73</citedby><cites>FETCH-LOGICAL-c299t-9b655d0710ce3f665a5c2a2e61742d2a76997c72d5979e35161c8aa52b7f48c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(82)90441-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,885,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7125199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5581296$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Godinho, O.E.S.</creatorcontrib><creatorcontrib>Aleixo, L.M.</creatorcontrib><creatorcontrib>Hora Alves, J.P.</creatorcontrib><title>Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme</title><title>Anal. Biochem.; (United States)</title><addtitle>Anal Biochem</addtitle><description>The potentiometric acid-base titration curve of fully protonated lysozyme at ionic strengths of 0.10 and 1.0
m has been performed. The stoichiometry and the p
K
a values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with p
K
a values of 3.76 and 5.02, the imidazole group with p
K
a 7.37 and the amine group with p
K
a 9.63, have been identified at an ionic strength of 0.10
m at 25.0°C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base.</description><subject>400102 - Chemical & Spectral Procedures</subject><subject>AMINO ACIDS</subject><subject>AZOLES</subject><subject>CARBOXYLIC ACIDS</subject><subject>Chemical Phenomena</subject><subject>CHEMICAL REACTION KINETICS</subject><subject>Chemistry</subject><subject>ENZYMES</subject><subject>GLYCOSYL HYDROLASES</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>Hydrogen-Ion Concentration</subject><subject>HYDROLASES</subject><subject>IMIDAZOLES</subject><subject>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</subject><subject>KINETICS</subject><subject>LYSOZYME</subject><subject>MOLECULAR STRUCTURE</subject><subject>Muramidase - analysis</subject><subject>O-GLYCOSYL HYDROLASES</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>Osmolar Concentration</subject><subject>Potentiometry</subject><subject>REACTION KINETICS</subject><subject>STOICHIOMETRY</subject><subject>TITRATION</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1r3DAUFKUh2ab9By2IHkp68EaSLcm6BMKStguBXpqzkOXnRsWWHEkObO7537XrZY89Cc2beR8zCH2kZEsJFdeEkLJgQsmrmn1VpKpood6gDSVKFKQk6i3anCgX6F1KfwihtOLiHJ1LyjhVaoNed48mGpshuheTXfDY-Ba3MAOD8ysSOpxdjiabpgf8O4ZpTAs4xpDB-YSbA-6dB3PqcVIsHzvFZ0hbvN9v8e049s6ueA64P6TwchjgPTrrTJ_gw_G9RA_f7n7tfhT3P7_vd7f3hWVK5UI1gvOWSEoslJ0Q3HDLDANBZcVaZqRQSlrJWq6kgpJTQW1tDGeN7KrayvISfV77hpSdTtZlsI82eA82a85rypSYSV9W0nzf0wQp68ElC31vPIQp6XkW50zUM7FaiTaGlCJ0eoxuMPGgKdFLRHrxXy_-65rpfxFpNcs-HftPzQDtSXTMZK7frHWYnXh2EJdFwVtoXVz2bIP7_4C_Io6h5g</recordid><startdate>19820701</startdate><enddate>19820701</enddate><creator>Godinho, O.E.S.</creator><creator>Aleixo, L.M.</creator><creator>Hora Alves, J.P.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19820701</creationdate><title>Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme</title><author>Godinho, O.E.S. ; Aleixo, L.M. ; Hora Alves, J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-9b655d0710ce3f665a5c2a2e61742d2a76997c72d5979e35161c8aa52b7f48c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>400102 - Chemical & Spectral Procedures</topic><topic>AMINO ACIDS</topic><topic>AZOLES</topic><topic>CARBOXYLIC ACIDS</topic><topic>Chemical Phenomena</topic><topic>CHEMICAL REACTION KINETICS</topic><topic>Chemistry</topic><topic>ENZYMES</topic><topic>GLYCOSYL HYDROLASES</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>Hydrogen-Ion Concentration</topic><topic>HYDROLASES</topic><topic>IMIDAZOLES</topic><topic>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</topic><topic>KINETICS</topic><topic>LYSOZYME</topic><topic>MOLECULAR STRUCTURE</topic><topic>Muramidase - analysis</topic><topic>O-GLYCOSYL HYDROLASES</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>Osmolar Concentration</topic><topic>Potentiometry</topic><topic>REACTION KINETICS</topic><topic>STOICHIOMETRY</topic><topic>TITRATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Godinho, O.E.S.</creatorcontrib><creatorcontrib>Aleixo, L.M.</creatorcontrib><creatorcontrib>Hora Alves, J.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Anal. Biochem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Godinho, O.E.S.</au><au>Aleixo, L.M.</au><au>Hora Alves, J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme</atitle><jtitle>Anal. Biochem.; (United States)</jtitle><addtitle>Anal Biochem</addtitle><date>1982-07-01</date><risdate>1982</risdate><volume>123</volume><issue>2</issue><spage>244</spage><epage>248</epage><pages>244-248</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>The potentiometric acid-base titration curve of fully protonated lysozyme at ionic strengths of 0.10 and 1.0
m has been performed. The stoichiometry and the p
K
a values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with p
K
a values of 3.76 and 5.02, the imidazole group with p
K
a 7.37 and the amine group with p
K
a 9.63, have been identified at an ionic strength of 0.10
m at 25.0°C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7125199</pmid><doi>10.1016/0003-2697(82)90441-9</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Anal. Biochem.; (United States), 1982-07, Vol.123 (2), p.244-248 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74255268 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 400102 - Chemical & Spectral Procedures AMINO ACIDS AZOLES CARBOXYLIC ACIDS Chemical Phenomena CHEMICAL REACTION KINETICS Chemistry ENZYMES GLYCOSYL HYDROLASES HETEROCYCLIC COMPOUNDS Hydrogen-Ion Concentration HYDROLASES IMIDAZOLES INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY KINETICS LYSOZYME MOLECULAR STRUCTURE Muramidase - analysis O-GLYCOSYL HYDROLASES ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS Osmolar Concentration Potentiometry REACTION KINETICS STOICHIOMETRY TITRATION |
| title | Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme |
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